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Toxicological Risk Assessment of Zinc Oxide and Silver Nanoparticles in Food Packaging Cover

Toxicological Risk Assessment of Zinc Oxide and Silver Nanoparticles in Food Packaging

Open Access
|May 2026

References

  1. Barbato C. Nanoscienza e Nanotecnologia-La tossicità dei nanomateriali. Caserta: Pasquale Gnasso Editore, 2018.
  2. EFSA Scientific Committee. Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health. Efsa Journal 2021; 19: 1-111.
  3. EFSA Scientific Committee. Guidance on technical requirements for regulated food and feed product applications to establish the presence of small particles including nanoparticles. EFSA Journal 2021: 1-48.
  4. More S, et al. Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health. Efsa Journal 2021; 19: 1-111.
  5. Pistollato F, Madia F, Corvi R, Munn S, Grignard E, Paini A, Worth A, Bal-Price A, Prieto P, Casati S, Berggren E, Bopp SK, Zuang V. Current EU regulatory requirements for the assessment of chemicals and cosmetic products: challenges and opportunities for introducing new approach methodologies. Archives of Toxicology 2021; 95: 1867-1897.
  6. Kumar V, Sharma N, Maitra SS. In vitro and in vivo toxicity assessment of nanoparticles. International Nano Letters 2017; 7: 243-256.
  7. Daicoviciu, D, A Filip, RM Ion, S Clichici, N Decea, A Muresan, Oxidative photodamage induced by photodynamic therapy with methoxyphenyl porphyrin derivatives in tumour-bearing rats, Folia Biol.(Praha) 2011; 57 (1), 12-19.
  8. Espitia PJP, Soares NFF, Coimbra JSR, Andrade NJ, Cruz RS, Medeiro EAA. Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications. Food Bioprocess Technology 2012; 5: 1447-1464.
  9. Bancuta, OR, A Chilian, I Bancuta, RM Ion, R Setnescu, T Setnescu, ...Improvement of spectrophotometric method for determination of phenolic compounds by statistical investigations, Rom. Journ. Phys 2016, 61 (7-8), 1255-1264
  10. Fierascu, I, RM Ion, M Radu, IR Bunghez, SM Avramescu, RC Fierascu, Comparative study of antifungal effect of natural extracts and essential oils of Ocimum basilicum on selected artefacts, Rev. Roum. Chim 2014, 59 (3-4), 207-211
  11. Fierascu, RC, RM Ion, I Dumitriu, Noble metals nanoparticles synthesis in plant extracts, Journal of Optoelectronics and Advanced Materials 2010, 4 (9), 1297 - 1280
  12. Bancuta, OR, A Chilian, I Bancuta, RM Ion, R Setnescu, FT-IR And UV-VIS characterization of grape extracts used as antioxidants in polymers, Rev. Roum. Chim. 2015, 60 (5-6), 571-577
  13. Smaoui S, et al. Zinc oxide nanoparticles in meat packaging: A systematic review of recent literature. Food Packaging and Shelf Life 2023; 36: 1-20.
  14. Silva BL, et al. Relationship Between Structure and Antimicrobial Activity Of Zinc Oxide Nanoparticles: An Overview. International Journal of Nanomedicine 2019; 14: 9395–9410.
  15. Kim I, et al. ZnO Nanostructures in Active Antibacterial Food Packaging: Preparation Methods, Antimicrobial Mechanisms, Safety Issues, Future Prospects, and Challenges. Food Reviews International 2022; 38: 537-565.
  16. Paidari S, et al. Migration of Various Nanoparticles into Food Samples: A Review. Foods 2021; 10: 1-11.
  17. Choi SJ, Choy JH. Biokinetics of zinc oxide nanoparticles: toxicokinetics, biological fates, and protein interaction. International Journal of Nanomedicine 2014; 9: 261-269.
  18. Cardoso D, Narcy A, Durosoy S, Bordes C, Chevalier Y. Dissolution kinetics of zinc oxide and its relationship with physicochemical characteristics. Powder Technology 2021; 378: 746-759.
  19. Youn SM, Choi SJ. Food Additive Zinc Oxide Nanoparticles: Dissolution, Interaction, Fate, Cytotoxicity, and Oral Toxicity. International Journal of Molecular Sciences 2022; 23: 1-18.
  20. Tian L, et al. Neurotoxicity induced by zinc oxide nanoparticles: age-related differences and interaction. Scientific Reports 2015; 5: 1-12.
  21. Ansar S, Abudawood M, Hamed SS, Aleem MM. Exposure to Zinc Oxide Nanoparticles Induces Neurotoxicity and Proinflammatory Response: Amelioration by Hesperidin. Biological Trace Element Research 2017; 175: 360-366.
  22. Sharma VK, Filip J, Zborilb R, Varma RS. Natural inorganic nanoparticles – formation, fate, and toxicity in the environment. Chemical Society Reviews 2015; 44: 8410--8423.
  23. Dinesh R, Anandaraj M, Srinivasan V, Hamza S. Engineered nanoparticles in the soil and their potential implications to microbial activity. Geoderma 2012; 173-174: 19-27.
  24. EFSA Panel (CEF). Safety assessment of the substance zinc oxide, nanoparticles, for use in food contact materials. Efsa Journal 2016; 14: 1-8.
  25. Parida C, Malik GK, Mitra J. Preparation and characterization of zinc oxide nanoparticle, its migration, and toxicity evaluation. Journal of Food Processing and Preservation 2022: 1-13.
  26. Silva FAGS, et al. Performance of bacterial nanocellulose packaging film functionalised in situ with zinc oxide: Migration onto chicken skin and antimicrobial activity. Food Packaging and Shelf Life 2023; 39: 1-11.
  27. Xiao Y, Liu Y, Kang S, Wang K, Xu H. Development and evaluation of soy protein isolate-based antibacterial nanocomposite films containing cellulose nanocrystals and zinc oxide nanoparticles. Food Hydrocolloids 2020; 106: 105898.
  28. Bumbudsanpharoke N, Choi J, Park HJ, Ko S. Zinc migration and its effect on the functionality of a low density polyethylene-ZnO nanocomposite film. Food Packaging and Shelf Life 2019; 20: 100301.
  29. Kavuncuoglu H, Yalcin H, Dogan M. Development of (TiO2-ZnO)/LDPE based active nanocomposite films and detection of migration to minced beef during storage using response surface methodology. Food Chemistry 2023; 402: 134278.
  30. De Jong WH, et al. Systemic and immunotoxicity of silver nanoparticles in an intravenous 28 days repeated dose toxicity study in rats. Biomaterials 2013; 34: 8333-8343.
  31. Amooaghaie R, Saeri MR, Azizi M. Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles. Ecotoxicology and Environmental Safety 2015; 120: 400-408.
  32. Fierascu, I, IR Bunghez, RC Fierascu, RM Ion, CE Dinu Pîrvu, D Nuta, Characterization and antioxidant activity of phytosynthesised silver nanoparticles using Calendula officinalis extract, Farmacia 62 (1), 2014, 129-136
  33. Sriram MI, et al. Size-based cytotoxicity of silver nanoparticles in bovine retinal endothelial cells. Nanoscience Methods 2012; 1: 56-77.
  34. Akter M, et al. A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. Journal of Advanced Research 2018; 9: 1-16.
  35. Ricardo AIC, et al. Screening-confirmation strategy for nanomaterials involving spectroscopic analytical techniques and its application to the control of silver nanoparticles in pastry samples. Spectrochimica Acta Part A 2021; 246: 119015.
  36. Mackevica A, Olsson ME, Hansen SF. Silver nanoparticle release from commercially available plastic food containers into food simulants. Journal of Nanoparticle Research 2016; 18.
  37. Metak AM, Nabhani F, Connolly SN. Migration of engineered nanoparticles from packaging into food products. LWT - Food Science and Technology 2015; 64: 781-787.
  38. Ntim SA, Thomas TA, Begley TH, Noonan GO. Characterisation and potential migration of silver nanoparticles from commercially available polymeric food contact materials. Food Additives & Contaminants: Part A 2015: 1003-1011.
  39. Cushen M, et al. Evaluation and Simulation of Silver and Copper Nanoparticle Migration from Polyethylene Nanocomposites to Food and an Associated Exposure Assessment. Journal of Agricultural and Food Chemistry 2014; 62: 1403-1411.
  40. Kim MH, et al. Kinetic and thermodynamic studies of silver migration from nanocomposites. Journal of Food Engineering 2019; 243: 1-8.
  41. Nair SB, Alummoottil NJ, Moothandasserry SS. Chitosankonjac glucomannan-cassava starch-nanosilver composite films with moisture resistant and antimicrobial properties for food-packaging applications. Starch 2016.
  42. Marchiore NG, et al. Migration evaluation of silver nanoparticles from antimicrobial edible coating to sausages. LWT - Food Science and Technology 2017; 76: 203-208.
  43. El Mouzahim M, et al. Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation. Food Chemistry 2023; 410: 135470.
  44. Amiri N, et al. Characterizing silver nanoparticles in beverages and following their release from silver-containing food containers using sector field single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). Measurement: Food 2022; 8: 100061.
  45. Ortega F, Sobral P, Jios JL, Arce VB, García MA. Starch Nanocomposite Films: Migration Studies of Nanoparticles to Food Simulants and Bio-Disintegration in Soil. Polymers 2022; 14: 1-17.
  46. Motelica L, et al. Antibacterial Biodegradable Films Based on Alginate with Silver Nanoparticles and Lemongrass Essential Oil–Innovative Packaging for Cheese. Nanomaterials 2021; 11: 1-22.
  47. EFSA Journal. Safety assessment of the substance silver nanoparticles for use in food contact materials. Efsa Journal 2021: 1-11.
DOI: https://doi.org/10.2478/bsmm-2026-0003 | Journal eISSN: 2537-3161 | Journal ISSN: 1844-1076
Language: English
Page range: 18 - 24
Published on: May 12, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2026 Daniela Grigorescu, Rodica Mariana Ion, published by Valahia University of Targoviste
This work is licensed under the Creative Commons Attribution 4.0 License.