Table 1
Oligonucleotides used for nodC amplification
Tabelle 1. Für die nodC-Amplifikation verwendete Oligonukleotide
| Primer | 5′–3′ nucleotide sequence |
|---|---|
| nodCF | AYGTHGTYGAYGACGGTTC |
| nodCFu | AYGTHGTYGAYGACGGITC |
| nodCF2 | AYGTHGTYGAYGACGGCTC |
| nodCF4 | AYGTHGTYGAYGACGGATC |
| nodCFn | AGGTGGTYGAYGACGGTTC |
| nodCI | CGYGACAGCCANTCKCTATTG |
[i] Y = C or T; H = A, C or T; R = A or G; S = C or G; K = G or T; N = A, C, G or T; I = inosine
Table 2
The mean numbers of colony forming units (CFUs) of bacteria colonizing pea nodules per one nodule depending on variants (pea varieties and inoculants).
Tabelle 2. Die Durchschnittswerte koloniebildender Einheiten (KBE) von Bakterien, die Erbsenknollen besiedeln, pro Knöllchen in Abhängigkeit der Varianten (Erbsensorten und Inokulen).
| Variant | Nodules | ||||
|---|---|---|---|---|---|
| Large cream | Small cream | Large red | Small red | Mean | |
| Klif (uninoculated) | 20.5 | 87.0 | 128.5 | 52.0 | 72.0b |
| Tarchalska (uninoculated) | 6.0 | 110.0 | 157.5 | 36.0 | 77.4b |
| Klif + IUNG | 84.0 | 97.5 | 310.0 | 212.0 | 175.9a |
| Tarchalska + IUNG | 110.0 | 45.0 | 101.0 | 49.0 | 76.3b |
| Klif + NitragineTM | 54.0 | 79.5 | 67.0 | 39.0 | 59.9b |
| Tarchalska + NitragineTM | 45.0 | 54.0 | 32.0 | 53.0 | 46.0b |
| Mean | 53.2b | 78.8ab | 132.7a | 73.5ab | |
[i] Pairs of treatments that are not significantly different according to the LSD test (p<0.05) share the same letter.
Figure 1
BamHI digestion of nodC gene of 17 selected Rhizobium leguminosarum samples. Letters at the top of the figure show experimental variants, whereas letters at the bottom of the figure show the digestion patterns.
Legend for the top of the figure: Ku, uninoculated “Klif ” pea plants; KI, “Klif ” plants inoculated with IUNG; KN, “Klif ” plants inoculated with Nitragine™; Tu, uninoculated “Tarchalska” pea plants; TI, “Tarchalska” plants inoculated with IUNG; TN, “Tarchalska” plants inoculated with Nitragine™; IUNG, bacterial isolates derived from the IUNG inoculant; LR, LC, SR, and SC refer to the types of nodules: LR, large red; LC, large cream; SR, small red; SC, small cream.
Legend for the bottom of the figure: M, GeneRuler™ 1kb DNA Ladder (Thermo Scientific); A, B, and C, clear digestion profiles; A + B, mixed digestion profiles.
Abbildung 1. BamHI-Verdauung des nodC-Gens von 17 ausgewählten Proben von Rhizobium leguminosarum. Buchstaben oben in der Abbildung zeigen experimentelle Varianten, während Buchstaben unten in der Abbildung die Verdauungsmuster zeigen.
Table 3
Identification of bacteria colonizing pea nodules
Tabelle 3. Identifizierung der Bakterien, welche die Erbsenknöllchen besiedelten
| Variant | Bacteria species | Maximum identity (%) |
|---|---|---|
| IUNG | Rhizobium leguminosarum bv. viciae USDA 2370 strain USDA 2370; ATCC 10004 | 99 |
| NitragineTM | Achromobacter insolitus strain LMG 6003 | 99 |
| Klif (uninoculated) | Bacillus megaterium strain IAM 13418 | 99 |
| Tarchalska (uninoculated) | Stenotrophomonas maltophilia strain IAM 12423 | 99 |
| Tarchalska + IUNG | Stenotrophomonas maltophilia strain IAM 12423 | 99 |
| Klif + NitragineTM | Stenotrophomonas maltophilia strain IAM 12423 | 98 |
| Tarchalska + NitragineTM | Stenotrophomonas maltophilia strain IAM 12423 | 99 |
Table 4
Detection of nodC gene in the studied bacterial isolates
Tabelle 4. Nachweis des nodC-Gens in den untersuchten Bakterienisolaten
| Isolate No. | Variant | Nodule | Number of isolates per variant | nodC presence |
|---|---|---|---|---|
| 1 | Klif (uninoculated) | Large red | 1 | Yes |
| 2 | Klif (uninoculated) | Large red | 2 | No |
| 3 | Klif (uninoculated) | Small red | 1 | No |
| 4 | Klif (uninoculated) | Small red | 2 | No |
| 5 | Klif (uninoculated) | Large cream | 1 | Yes |
| 6 | Klif (uninoculated) | Small cream | 1 | Yes |
| 7 | Klif (uninoculated) | Small cream | 2 | No |
| 8 | Tarchalska (uninoculated) | Large red | 1 | Yes |
| 9 | Tarchalska (uninoculated) | Small red | 1 | Yes |
| 10 | Tarchalska (uninoculated) | Small red | 2 | No |
| 11 | Tarchalska (uninoculated) | Large cream | 1 | Yes |
| 12 | Tarchalska (uninoculated) | Small cream | 2 | Yes |
| 13 | Klif + IUNG | Large red | 1 | Yes |
| 14 | Klif + IUNG | Small red | 1 | Yes |
| 15 | Klif + IUNG | Large cream | 1 | Yes |
| 16 | Klif + IUNG | Small cream | 1 | No |
| 17 | Tarchalska + IUNG | Large red | 1 | Yes |
| 18 | Tarchalska + IUNG | Large red | 2 | No |
| 19 | Tarchalska + IUNG | Large red | 3 | No |
| 20 | Tarchalska + IUNG | Large red | 4 | No |
| 21 | Tarchalska + IUNG | Small red | 1 | No |
| 22 | Tarchalska + IUNG | Small red | 2 | No |
| 23 | Tarchalska + IUNG | Small red | 3 | No |
| 24 | Tarchalska/IUNG | Large cream | 1 | No |
| 25 | Tarchalska + IUNG | Large cream | 2 | No |
| 26 | Tarchalska + IUNG | Large cream | 3 | No |
| 27 | Tarchalska + IUNG | Small cream | 1 | No |
| 28 | Klif + Nitragine™ | Large red | 1 | No |
| 29 | Klif + Nitragine™ | Large red | 2 | No |
| 30 | Klif + Nitragine™ | Large red | 3 | No |
| 31 | Klif + Nitragine™ | Large red | 4 | No |
| 32 | Klif + Nitragine™ | Small red | 1 | Yes |
| 33 | Klif + Nitragine™ | Small red | 2 | No |
| 34 | Klif + Nitragine™ | Large cream | 1 | Yes |
| 35 | Klif + Nitragine™ | Small cream | 1 | Yes |
| 36 | Tarchalska/Nitragine™ | Large red | 1 | Yes |
| 37 | Tarchalska + Nitragine™ | Large red | 2 | No |
| 38 | Tarchalska + Nitragine™ | Small red | 1 | Yes |
| 39 | Tarchalska + Nitragine™ | Large cream | 1 | Yes |
| 40 | Tarchalska + Nitragine™ | Small cream | 1 | Yes |
| 41 | Tarchalska + Nitragine™ | Small cream | 2 | Yes |
| 42 | Tarchalska/Nitragine™ | Small cream | 3 | Yes |
| 43 | Tarchalska + Nitragine™ | Small cream | 4 | Yes |
| 44 | Nitragine™ | Nonapplicable | 1 | No |
| 45 | IUNG | Nonapplicable | 1 | Yes |
| 46 | R. leguminosarum bv. viceae DSM 30143 | Nonapplicable | 1 | No |