Table 1.
PCR primer sequences for TNF, NLRP3, IL-1B, and β-actin
| TNF | F: CCTCTCTCTAATCAGCCCTCTG R: GAGGACCTGGGAGTAGATGAG |
| NLRP3 | F: GATCTTCGCTGCGATCAACAG R: CGTGCATTATCTGAACCCCAC |
| IL-1B | F: TTCGACACATGGGATAACGAGG R: TTTTTGCTGTGAGTCCCGGAG |
| β-actin | F: CATGTACGTTGCTATCCAGGC R: CTCCTTAATGTCACGCACGAT |
Figure 1.
Volcano plot of common DEGs.
Figure 2.
Heatmap of top common DEGs.
Figure 3.
GSEA of common DEGs.
Figure 4.
Cross-validation curve of the LASSO regression model.
Figure 5.
Coefficient profile plot of the LASSO regression model.
Figure 6.
SVM-RFE algorithm ROC curve.
Figure 7.
RF algorithm ROC curve.
Figure 8.
Intersection of the three machine learning algorithms identified one key genes: SRGN.
Figure 9.
PPI network of common DEGs identified in ITP.
Table 2.
Highly connected DEG nodes identified in the PPI network
| Name of DEG | log2|FC| | P value | Number of connections |
|---|---|---|---|
| GAPDH | 3.2953409195274 | 2.19422420641149e-07 | 110 |
| TNF | 3.68646049371584 | 1.47314840083729e-10 | 105 |
| IL1B | 2.50243396726262 | 3.13683399045308e-12 | 75 |
| CD8A | 2.86640582723886 | 7.27565725241324e-06 | 71 |
| STAT3 | 2.09730201355131 | 4.96438557174655e-06 | 70 |
| UBA52 | 4.92834353505914 | 5.23423339042131e-08 | 63 |
| UBC | 2.18610712682655 | 1.05952355437303e-05 | 60 |
| RPS3 | 2.79618429621907 | 3.63790536504739e-08 | 56 |
| UBB | 3.82095799888894 | 4.31876503261331e-08 | 56 |
| CYCS | 2.29957812821002 | 5.45213130618771e-07 | 50 |
| CCL5 | 3.55169552386934 | 3.71390385817605e-09 | 49 |
| TYROBP | 5.2780755168582 | 5.38420777664558e-13 | 46 |
| COX4I1 | 2.09433418305494 | 4.71041649541531e-06 | 39 |
| H3-3B | 22.5682455453967 | 6.62776003598768e-07 | 39 |
| ISG15 | 3.35218542157055 | 3.0642442177228e-07 | 38 |
| TNFRSF1A | 3.60578976971292 | 1.90860747716885e-09 | 38 |
| CTSS | 3.17044562984854 | 8.53715947857592e-06 | 37 |
Figure 10.
mRNA Expression of TNF.
Figure 11.
mRNA Expression of NLRP3.
Figure 12.
mRNA Expression of IL1B.
Figure 13.
Venn diagram of overlapping genes between ITP DEGs and CKD associated genes.
Table 3.
Details of 39 overlapping genes between ITP and CKD
| RPL22 | ISG15 | HPCA | FNDC5 | KIF1B | PADI3 | TP73 | PRDM16 | CHD5 |
| RUNX3 | EFHD2 | PINK1 | CNR2 | ALPL | CA6 | RS1 | PDPN | CORT |
| TNF | PTAFR | SYF2 | SLC45A1 | TRNP1 | VWA1 | TINAGL1 | HSPG2 | CLCNKB |
| FGR | TNFRSF25 | AGTRAP | PADI4 | PHACTR4 | ZNF683 | TRIM63 | GABRD | NMNAT1 |
| ESPN | NPPB | EPHA2 |
Figure 14.
Venn diagram of overlapping genes between ITP DEGs and MN associated genes.
Table 4.
Details of 24 overlapping genes between ITP and MN
| TNF | ISG15 | KIF17 | KIAA0319L | PTAFR | PODN | LRP8 |
| WASF2 | FNDC5 | ALPL | RAB3B | HSPG2 | ESPN | PINK1 |
| EFHD2 | COL8A2 | PDPN | TRABD2B | TNFRSF25 | KCNQ4 | BSND |
| FOXE3 | EPHA2 | CLCNKB |
Figure 15.
Venn diagram of overlapping genes between ITP DEGs and IgAN associated genes.
Table 5.
Details of 37 overlapping genes between ITP and IgAN
| NLRP3 | PC | WDR19 | NPHS2 | COL4A4 | IL1B | EPO |
| RUNX3 | IL1RN | FCGR2B | COL4A3 | CD14 | COL5A1 | CFB |
| PSMB8 | GTF2H5 | PROC | SFRP1 | IL33 | THSD7A | RAB3B |
| TNF | HFE | UNC13B | IL6R | INVS | HGF | LMX1B |
| IL4 | IKZF2 | PPARG | ROBO2 | REN | RARRES1 | PRDM16 |
| PLA2R1 |
Figure 16.
PPI network of overlapping genes between ITP and CKD
Figure 17.
PPI network of overlapping genes between ITP and MN
Figure 18.
PPI network of overlapping genes between ITP and IgAN