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Vitamin D receptor polymorphisms among the Turkish population are associated with multiple sclerosis

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Open Access
|Mar 2023

Figures & Tables

Genotype distribution of VDR Gene Bsm-I polymorphisms in different inheritance models in the MS patients and control group

a) Bsm-I Recessive Model
G/G or G/AA/ATotalp
MS-Control
Control83.3% (n=169)16.7% (n=34)100% (n=203)
MS80.1% (n=217)19.9% (n=54)100% (n=271)0.379
Total81.4%(n=386)18.6% (n=88)100% (n=474)
b) Bsm-I Dominant Model
G/GG/A or A/ATotalp
MS-Control
Control36.9% (n=75)63.1% (n=128)100% (n=203)
MS35.8% (n=97)64.2% (n=174)100% (n=271)0.796
Total36.3%(n=172)63.7% (n=302)100% (n=474)
c)Bsm-I Homozygote Model
G/GA/ATotalp
MS-Control
Control68.8% (n=75)31.2% (n=34)100% (n=88)
MS64.2% (n=97)35.8% (n=54)100% (n=172)0.442
Total33.8%(n=88)66.2% (n=172)100% (n=260)
d)Bsm-I Heterozygote Model
G/GG/ATotalp
MS-Control
Control44.4% (n=75)55.6% (n=94)100% (n=169)
MS44.7% (n=97)55.3% (n=120)100% (n=217)0.950
Total55.4%(n=214)44.6% (n=172)100% (n=386)

Primers used for amplification of polymorphism sites on the VDR gene_

PolymorphismForward PrimerReverse Primer
Fok-I (rs2228570)AGGATGCCAGCTGGCCCTGGCACTGGCTGTGAGCGCCGCATGTTCCATG
Bsm-I (rs1544410)TCCTTGAGCCTCCAGTCCAGGGCAACCTGAAGGGAGACGTAGC
Taq-I (rs731236)AGAGCATGGACAGGGAGCAAGGCTAGCTTCATGCTGCACTCAGGCTGG

Genotype distribution of VDR Gene Fok-I polymorphisms in different inheritance models in the MS patients and control group

a)Fok-I Recessive Model
T/C or TTC/CTotalp
MS-Control
Control52.2% (n=106)47.8% (n=97)100% (n=203)
MS58.3% (n=158)41.7% (n=113)100% (n=271)0.187
Total55.7%(n=264)44.3% (n=210)100% (n=474)
b)Fok-I Dominant Model
T/TT/C or C/CTotalp
MS-Control
Control13.3% (n=27)86.7% (n=176)100% (n=203)
MS22.5% (n=61)77.5% (n=210)100% (n=271)0.011
Total18.6%(n=88)81.4% (n=386)100% (n=474)
c)Fok-I Homozygote Model
T/TC/CTotalp
MS-Control
Control21.8% (n=27)78.2% (n=97)100% (n=124)
MS35.1% (n=61)64.9% (n=113)100% (n=174)0.013
Total29.5%(n=88)70.5% (n=210)100% (n=298)
d)Fok-I Heterozygote Model
T/TT/CTotalp
MS-Control
Control25.5% (n=27)74.5% (n=79)100% (n=106)
MS38.6% (n=61)61.4% (n=97)100% (n=158)0.026
Total33.8%(n=88)66.7% (n=176)100% (n=264)

Distribution of VDR gene Polymorphisms within gender in MS patients

a)Bsm-I Genotype
G/GG/AA/ATotalp
Gender
Female37.2% (n=70)43.6% (n=82)19.1% (n=36)100% (n=188)
Male32.5% (n=27)45.8% (n=38)21.7% (n=18)100% (n=83)0.740
Total35.8% (n=97)44.3% (n=120)19.9% (n=54)100% (n=271)
b)Fok-I Genotype
T/TT/CC/CTotalp
Gender
Female22.3% (n=42)33.0% (n=62)44.7% (n=84)100% (n=188)
Male22.9% (n=19)42.2% (n=35)34.9% (n=29)100% (n=83)0.262
Total22.5% (n=61)35.8% (n=97)41.7% (n=113)100% (n=271)
c)Taq-I Genotype
C/CC/TT/TTotalp
Gender
Female34.6% (n=65)41.0% (n=77)24.5% (n=46)100% (n=188)
Male28.9% (n=24)39.8% (n=33)31.3% (n=26)100% (n=83)0.449
Total32.8% (n=89)40.6% (n=110)26.6% (n=72)100% (n=271)

Genotype distribution and allele frequency of VDR Fok-I polymorphism in MS patients and healthy controls_

a)Fok-I Genotype
T/TT/CC/CTotalpPower (%)
MS-Control
Control13.3% (n=27)38.9% (n=79)47.8% (n=97)100%(n=203)0.037
MS22.5% (n=61)35.8% (n=97)41.7% (n=113)100%(n=271) 100
Total18.6% (n=88)37.1% (n=176)44.3% (n=210)100%(n=474)
MS subtypes
Control13.3% (n=27)38.9% (n=79)47.8% (n=97)100%(n=203)
RRMS SPMS23.3% (n=43) 21.1% (n=18)33.2% (n=61) 42.4% (n=36)43.5% (n=80) 36.5% (n=31)100%(n=184) 100%(n=85)0.074100
PPMS0.0% (n=0)0.0% (n=0)100%.0 (n=2)100%(n=2)
Total18.6% (n=88)37.1% (n=176)44.3% (n=210)100%(n=474)
b)Fok-I Allele
TCTotalpPower (%)
MS-Control
Control32.8% (n=133)67.2% (n=273)100% (n=406)
MS40.4% (n=219)59.6% (n=323)100% (n=542)0.016100
Total37.1% (n=352)62.9% (n=596)100% (n=948)
MS subtypes
Control32.8% (n=133)67.2% (n=273)100% (n=406)
RRMS39.9% (n=147)60.1% (n=221)100% (n=368)
SPMS42.4% (n=72)57.6% (n=98)100% (n=170)0.030100
PPMS0.0% (n=0)100.0% (n=4)100% (n=4)
Total37.1% (n=352)62.9% (n=596)100% (n=948)

Genotype distribution of VDR Gene Taq-I polymorphisms in different inheritance models in the MS patients and control group

a)Taq-I Recessive Model
T/C or T/TC/CTotalp
MS-Control
Control62.6% (n=127)37.4% (n=76)100% (n=203)
MS67.2% (n=182)32.8% (n=89)100% (n=271)0.299
Total65.2%(n=309)34.8% (n=165)100% (n=474)
b)Taq-I Dominant Model
T/TT/C or C/CTotalp
MS-Control
Control16.7% (n=34)83.3% (n=169)100% (n=203)
MS26.6% (n=72)73.4% (n=199)100% (n=271)0.011
Total22.4%(n=106)77.6% (n=368)100% (n=474)
c)Taq-I Homozygote Model
T/TC/CTotalp
MS-Control
Control30.9% (n=34)69.1% (n=76)100% (n=110)
MS44.7% (n=72)55.3% (n=89)100% (n=161)0.022
Total39.1%(n=106)60.9% (n=165)100% (n=271)
d)Taq-I Heterozygote Model
T/TT/CTotalp
MS-Control
Control26.8% (n=34)73.2% (n=93)100% (n=127)
MS39.6% (n=72)60.4% (n=110)100% (n=182)0.020
Total34.3%(n=106)65.7% (n=203)100% (n=309)

Genotype distribution and allele frequency of VDR Taq-I polymorphism in MS patients and healthy controls_

a)Taq-I Genotype
C/CC/TT/TTotalpPower (%)
MS-Control
Control16.7% (n=34)45.8% (n=93)37.5% (n=76)100%(n=203)0.040
MS26.6% (n=72)40.6% (n=110)32.8% (n=89)100%(n=271) 99
Total22.4% (n=106)42.8% (n=203)34.8% (n=165)100%(n=474)
MS subtypes
Control16.7% (n=34)45.8% (n=93)37.5% (n=76)100%(n=203)
RRMS23.9% (n=44)43.5% (n=80)32.6% (n=60)100%(n=184)
SPMS32.9% (n=28)34.2% (n=29)32.9% (n=28)100%(n=85)0.101100
PPMS0.0% (n=0)50.0% (n=1)50.0% (n=1)100%(n=2)
Total22.4% (n=106)42.8% (n=203)34.8% (n=165)100%(n=474)
b)Taq-I Allele
TCTotalpPower (%)
MS-Control
Control60.3% (n=245)39.7% (n=161)100% (n=406)0.027
MS53.1% (n=288)46.9% (n=254)100% (n=542) 99
Total56.2% (n=533)43.8% (n=415)100% (n=948)
MS subtypes
Control60.3% (n=245)39.7% (n=161)100% (n=406)
RRMS54.3% (n=200)45.7% (n=168)100% (n=368)
SPMS50.0% (n=85)50.0% (n=85)100% (n=170)0.087100
PPMS75.0% (n=3)25.0% (n=1)100% (n=4)
Total56.2% (n=533)43.8% (n=415)100% (n=948)

Distribution of VDR gene allele within gender in MS patients

a) Bsm-I Allele
GATotalp
Gender
Female61.7% (n=153)38.3% (n=95)100% (n=248)
Male48.0% (n=49)52.0% (n=53)100% (n=102)0.019
Total57.7%(n=202)42.3% (n=148)100% (n=350)
b) Fok-I Allele
TCTotalp
Gender
Female29.0% (n=72)71.0% (n=176)100% (n=248)0.800
Male30.4% (n=31)71.0% (n=69.6)100% (n=102)
Total55.7%(n=264)70.6% (n=247)100% (n=350)
c) Taq-I Allele
TCTotalp
Gender
Female37.5% (n=93)37.4% (n=76)100% (n=248)0.137
Male46.1% (n=47)53.9% (n=55)100% (n=102)
Total40.0%(n=140)60.0% (n=210)100% (n=350)

Genotype distribution and allele frequency of VDR Bsm-I polymorphism in MS patients and healthy controls_

a)Bsm-I Genotype
G/GG/AA/ATotalpPower (%)
MS-Control
Control37.0% (n=75)46.3% (n=94)16.7% (n=34)100% (n=203)0.677
MS35.8% (n=97)44.3% (n=120)19.9% (n=54)100% (n=271) 100
Total36.3% (n=172)45.1% (n=214)18.6% (n=88)100% (n=474)
MS subtypes
Control37.0% (n=75)46.3% (n=94)16.7% (n=34)100% (n=203)
RRMS35.9% (n=66)46.7% (n=86)17.4% (n=32)100% (n=184)
SPMS35.3% (n=30)38.8% (n=33)25.9% (n=22)100% (n=85)0.622100
PPMS50.0% (n=1)50.0% (n=1)0.0% (n=0)100% (n=2)
Total36.3% (n=172)45.1% (n=214)18.6% (n=88)100% (474)
b)Bsm-I Allele
GATotalpPower (%)
MS-Control
Control60.1% (n=244)39.9% (n=162)100% (n=406)
MS57.9% (n=314)42.1% (n=228)100% (n=542)0.503100
Total58.9%(n=558)41.1% (n=390)100% (n=948)
MS subtypes
Control60.1% (n=244)39.9% (n=162)100% (n=406)
RRMS59.2% (n=218)40.8% (n=150)100% (n=368)0.589
SPMS54.7% (n=93)45.3% (n=77)100% (n=170) 100
PPMS75.0% (n=3)25.0% (n=1)100% (n=4)
Total58.8% (n=558)41.2% (n=390)100% (n=948)
Language: English
Page range: 41 - 50
Published on: Mar 1, 2023
Published by: Macedonian Academy of Sciences and Arts
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2023 B Bulan, AY Hoscan, SN Keskin, A Cavus, EA Culcu, N Isik, EO List, A Arman, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.