Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

Dahdouh, Amel; Bachir-Bey, Mostapha; Kati, Djamel Edine

doi:10.2478/aucft-2020-0017

Abstract

The present study is focused on the determination of optimized activity conditions of peroxidase (POD) of turnip (Brassica rapa). The enzyme is extracted from turnip with 0.5M Na₂HPO₄ (pH7.0). Peroxidase activity is evaluated by monitoring the oxidation of guaiacol to tetraguaiacol in the presence of hydrogen peroxide (H₂O₂). Before the optimization of peroxidase activity from a turnip by response surface methodology (RSM), a preliminary step is conducted for each studied parameter (pH, temperature, and NaCl concentration). Further, RSM following Box-Behnken design is used in order to refine the optimization of the conditions. Response surface analysis showed that parameter values with the highest peroxidase activity are 5.9, 29.8°C, and 1.07M for pH, temperature, and NaCl, respectively. Under these experimental conditions, peroxidase activity is found to be 4027.25U/mg. The obtained experimental value agrees with that of the calculated one, indicating the pertinence of the developed model for the optimization of peroxidase activity.

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Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

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