Ultrastructural changes in postcapillary-sized venule morphology in aged mouse periodontal ligament

Cameron, J.; Sims, M. R.; Sampson, W. J.

doi:10.2478/aoj-2001-0002

Abstract

The aim of this study was to compare the postcapillary-sized venule (PCV) morphology of four young ALCA mice (35 days) and four colony-related aged mice (365 days) using the transmission electron microscope (TEM). Right and left mandibular first molar mesial roots with associated periodontal ligament (PDL) and bony socket, were used for TEM assessment. Five PCV profiles were selected at each 160µm interval, from the alveolar crest to the tooth apex. PCV profile dimensions were measured on standardised micrographs magnified x2900. Age affects were tested using multiple regression analysis. The number of PCV profiles in the tooth third of the PDL was higher in aged mice (p < 0.01) and comprised predominantly apericytic vessels (p < 0.001). The number of PCV profiles increased significantly (p < 0.001) in aged mice in the PDL middle circumferential third halfway down the molar root. Age had no significant affect on PCV diameter. Aged PDL permeability studies are needed to investigate whether the changes in aged PCV profile number are associated with functional modification of the PDL microvasculature.

References

Sheiham A. Dentistry for an ageing population: responsibilities and future trends. Dent Update 1990; 17: 70–4.
Search in Google Scholar Back to article
Clough G. Relationship between microvascular permeability and ultrastructure. Prog Biophys Molec Biol 1991; 55: 47–69.
Search in Google Scholar Back to article
Cook JJ, Wailgum TD, Vasthare US, Mayrovitz HN, Tuma RF. Age-related alterations in the arterial microvasculature of skeletal muscle. J Gerontol 1992; 47: B83–8.
Search in Google Scholar Back to article
Seidman MD, Khan MJ, Dolan DF, Quirk WS. Age-related differences in Cochlear microcirculation and auditory Brain Stem response. Arch Otolaryngol Neck Surg 1996; 122: 1221–6.
Search in Google Scholar Back to article
Simionescu M, Simionescu N. Ultrastructure of the microvascular wall: functional correlations. In: Renkin EM, Michel CC, eds. Handbook of Physiology, Section II: The Cardiovascular System, Microcirculation, Part 1. Baltimore, American Physiological Society, 1984; 41–101.
Search in Google Scholar Back to article
Duran-Reynals F. Age and infection: a review. J Gerontol 1946; 1: 358–73.
Search in Google Scholar Back to article
Wangensteen OD, Lysaker E, Savaryn P. Pulmonary capillary filtration and reflection coefficients in the adult rabbit. Microvasc Res 1977; 14: 81–97.
Search in Google Scholar Back to article
Matalon SV, Wangensteen OD. Pulmonary capillary filtration and reflection coefficients in the newborn rabbit. Microvasc Res 1977; 14: 99–110.
Search in Google Scholar Back to article
Hruza Z. Connective tissue. In: Kaley G, Altura BM, eds. Microcirculation. Baltimore, University Park Press. 1977; 167–83.
Search in Google Scholar Back to article
Berkovitz BKB, Moxham BJ, Newman HN. The periodontal ligament in health and disease. 2nd ed. London, Mosby-Wolfe, 1995.
Search in Google Scholar Back to article
Embery G. An update on the biochemistry of the periodontal ligament. Eur J Orthod 1990; 12: 77–80.
Search in Google Scholar Back to article
Moxham BJ, Evans, IL. The effects of ageing upon the connective tissues of the periodontal ligament. Connect Tissue Res 1995; 33: 31–5.
Search in Google Scholar Back to article
Moxham BJ, Webb PP, Benjamin M, Ralphs JR. Changes in the cytoskeleton of cells within the periodontal ligament and dental pulp of the rat first molar tooth during ageing. Eur J Oral Sci 1998; 106 (suppl 1): 376–83.
Search in Google Scholar Back to article
Lampugnani M, Caveda L, Breviario F, Maschio A, Dejana E. Endothelial cell-to-cell junctions: structural characteristics and functional role in the regulation of vascular permeability and leukocyte extravasation. Baillière's Clin Haem 1993; 6: 539–58.
Search in Google Scholar Back to article
Parlange L, Sims MR. A TEM stereological analysis of blood vessels and nerves in marmoset periodontal ligament following endodontics and magnetic incisor extrusion. Eur J Orthod 1993; 15: 33–44.
Search in Google Scholar Back to article
Rhodin JAG. Ultrastructure of mammalian venous capillaries, venules, and small collecting veins. J Ultrastruct Res 1968; 25: 452–500.
Search in Google Scholar Back to article
Sims, D. The pericyte: a review. Tissue Cell 1986; 18: 153–74.
Search in Google Scholar Back to article
Shepro D, Morel N. Pericyte physiology. FASEB J 1993; 7: 1031–8.
Search in Google Scholar Back to article
Freezer, S. A study of periodontal ligament mesial to the mouse mandibular first molar. MDS Thesis 1984; The University of Adelaide, South Australia.
Search in Google Scholar Back to article
Freezer S, Sims MR. A transmission electron-microscope stereological study of the blood vessels, oxytalan fibres and nerves of mouse molar periodontal ligament. Arch Oral Biol 1987; 32: 407–12.
Search in Google Scholar Back to article
Ott, L. An introduction to statistical methods and data analysis. Boston, PWS-Kent Publishing Company, 1988.
Search in Google Scholar Back to article
Sims MR. The microvascular venous pool and its ultra-structural associations in mouse molar periodontal ligament: periodontal microvasculature and nerves. Aust Orthod J 1983; 8: 21–7.
Search in Google Scholar Back to article
Cohen J. Weighted kappa: nominal scale agreement with provision for scaled disagreement or partial credit. Psychol Bull 1968; 70: 213–20.
Search in Google Scholar Back to article
Cameron J, Sims MR, Sampson WJ. Age changes in the periodontal ligament endothelial junctions of mice. J Dent Res 1995; 74: Abst 438.
Search in Google Scholar Back to article
Cooper S, Sims MR, Sampson WJ, Dreyer CW. A morphometric, electron microscopic analysis of tissue channels by ionic tracer in normal and tensioned rat molar apical periodontal ligament. Archs Oral Biol 1990; 35: 499–507.
Search in Google Scholar Back to article
Tang M, Sims MR. A TEM analysis of tissue channels in normal and orthodontically tensioned rat molar periodontal ligament. Eur J Orthod 1992; 14: 433–44.
Search in Google Scholar Back to article
Sims MR, Leppard PI, Sampson WJ, Dreyer CW. Microvascular luminal volume changes in aged mouse periodontal ligament. J Dent Res 1996; 75: 1503–11.
Search in Google Scholar Back to article

Ultrastructural changes in postcapillary-sized venule morphology in aged mouse periodontal ligament

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