Milk thistle (Silybum marianum) application in aquaculture: a phytogenic powerhouse for health and sustainability – a comprehensive review
References
- Abd El-Alim A.E.-A.F., El-Murr A., Hasan T. (2025). Benefits of silymarin as an immune and growth enhancer in farmed fish. Zagazig Vet. J., 53: 127–142.
- Abdelaziz R.A., Shukry M., Abdel-Latif H.M.R., Saleh R.M. (2023). Growth-promoting and immunostimulatory effects of phytobiotics as dietary supplements for Pangasianodon hypophthalmus fingerlings. Fish Shellfish Immunol., 133: 108531.
- Abdel-Latif H.M.R., Shukry M., Noreldin A.E., Ahmed H.A., El-Bahrawy A., Ghetas H.A., Khalifa E. (2023). Milk thistle (Silybum marianum) extract improves growth, immunity, serum biochemical indices, antioxidant state, hepatic histoarchitecture, and intestinal histomorphometry of striped catfish, Pangasianodon hypophthalmus. Aquaculture, 562: 738761.
- Abenavoli L., Izzo A.A., Milić N., Cicala C., Santini A., Capasso R. (2018). Milk thistle (Silybum marianum): A concise overview on its chemistry, pharmacological, and nutraceutical uses in liver diseases. Phytother. Res., 32: 2202–2213.
- Ahmadi K., Banaee M., Vosoghei A.R., Mirvaghefei A.R., Ataeimehr B. (2012). Evaluation of the immunomodulatory effects of silymarin extract (Silybum marianum) on some immune parameters of rainbow trout, Oncorhynchus mykiss (Actinopterygii: Salmoniformes: Salmonidae). Acta Ichthyol. Piscat., 42.
- Alagawany M., Farag M.R., Abdelnour S.A., Elnesr S.S. (2021). A review on the beneficial effect of thymol on health and production of fish. Rev. Aquacult., 13: 632–641.
- Al-Jubouri S.K.A., Al-Obaydi T.S.M. (2021). Effect of additives of Silybum marianum in some growth performance of common carp Cyprinus carpio L. Plant Arch., 21: 1162–1165.
- Al-Shawi S.G., Yousif A.Y., Al-Younis Z.K., Shichiyakh R.A., Zekiy A.O., Naserabad S.S. (2022). Dietary silymarin, Silybum marianum extract ameliorates cadmium chloride toxicity in common carp, Cyprinus carpio. Ann. Anim. Sci., 22: 741–750.
- Andrzejewska J., Martinelli T., Sadowska K. (2015). Silybum marianum: non-medical exploitation of the species. Ann. Appl. Biol., 167: 285–297.
- Anthony K.P., Saleh M.A. (2013). Free radical scavenging and antioxidant activities of silymarin components. Antioxidants, 2: 398–407.
- Ashley P.J. (2007). Fish welfare: Current issues in aquaculture. Appl. Anim. Behav. Sci., 104: 199–235.
- Asnan M., Muzammal U., Ehsan M., Iftikhar K., Sarwar M.I., Laiba G., Manan Z., Shah H., Qammar B., Tarteel M.H. (2024). Role of milk thistle (Silybum marianum) as a therapeutic feed additive in livestock, poultry and humans. In: Complementary and Alternative Medicine: Feed Additives, Abbas R.Z., Akhtar T., Asrar R., Khan A.M.A., Saeed Z. (eds). Unique Scientific Publishers, Faisalabad, Pakistan, pp. 51–58.
- Awad E., Awaad A. (2017). Role of medicinal plants on growth performance and immune status in fish. Fish Shellfish Immunol., 67: 40–54.
- Bahmani M., Shirzad H., Rafieian S., Rafieian-Kopaei M. (2015). Silybum marianum: beyond hepatoprotection. J. Evid.-Based Complementary Altern. Med., 20: 292–301.
- Banaee M., Sureda A., Mirvaghefi A.R., Rafei G.R. (2011). Effects of long-term silymarin oral supplementation on the blood biochemical profile of rainbow trout (Oncorhynchus mykiss). Fish Physiol. Biochem., 37: 885–896.
- Banaee M., Impellitteri F., Multisanti C.R., Sureda A., Arfuso F., Piccione G., Faggio C. (2023). Evaluating silymarin extract as a potent antioxidant supplement in diazinon-exposed rainbow trout: oxidative stress and biochemical parameter analysis. Toxics, 11: 737.
- Barad R.R., Verma D.K., Yusufzai S.I., Shrivastava V., Ram A.R. (2024). Herbal Feed Additives: Natural Boost for Aquatic Health and Growth, In: Sustainable Feed Ingredients and Additives for Aquaculture Farming: Perspectives from Africa and Asia, Gabriel N.N., Abasubong K.P., Erasmus V.N., Kamble M.T. (eds). Springer Nature Singapore, Singapore, pp. 405–431.
- Beg M.M., Roy S.M., Ramesh P., Moulick S., Tiyasha T., Bhagat S.K., Abdelrahman H.A. (2024). Organic aquaculture regulation, production, and marketing: current status, issues, and future prospects – a systematic review. Aquac. Res., 2024: 5521188.
- Bhattacharya S. (2020). Milk thistle seeds in health. In: Nuts and Seeds in Health and Disease Prevention (Second Edition), Preedy V.R., Watson R.R. (eds). Academic Press, pp. 429–438.
- Bijak M. (2017). Silybin, a major bioactive component of milk thistle (Silybum marianum L. Gaernt.) – chemistry, bioavailability, and metabolism. Molecules, 22: 1942.
- Cabello F.C., Godfrey H.P., Tomova A., Ivanova L., Dölz H., Millanao A., Buschmann A.H. (2013). Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ. Microbiol., 15: 1917–1942.
- Camini F.C., Costa D.C. (2020). Silymarin: not just another antioxidant. J. Basic Clin. Physiol. Pharmacol., 31.
- Chaklader M.R., Ahmed H.A., Khafaga A.F., Shukry M., Abo Selema T.A.M., Abdel-Latif H.M.R. (2024). Silybum marianum promotes growth, hepatic antioxidative activity, and splenic immunity but does not influence the intestinal barrier function of Nile tilapia, Oreochromis niloticus. Aquaculture, 583: 740554.
- Citarasu T. (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Aquac. Int., 18: 403–414.
- Datta S., Aggarwal D., Sehrawat N., Yadav M., Sharma V., Sharma A., Zghair A.N., Dhama K., Sharma A., Kumar V., Sharma A.K., Wang H. (2023). Hepatoprotective effects of natural drugs: Current trends, scope, relevance and future perspectives. Phytomedicine, 121: 155100.
- Dawood M.A.O., Koshio S., Esteban M.Á. (2018). Beneficial roles of feed additives as immunostimulants in aquaculture: a review. Rev. Aquacult., 10: 950–974.
- Dawood M.A.O., El Basuini M.F., Yilmaz S., Abdel-Latif H.M.R., Alagawany M., Kari Z.A., Abdul Razab M.K.A., Hamid N.K.A., Moonmanee T., Van Doan H. (2022). Exploring the roles of dietary herbal essential oils in aquaculture: a review. Animals, 12: 823.
- Eita A.A.B. (2022). Milk thistle (Silybum marianum (L.) Gaertn.): An overview about its pharmacology and medicinal uses with an emphasis on oral diseases. J. Oral Biosci., 64: 71–76.
- El-Houseiny W., Abd El-Hakim Y.M., Metwally M.M.M., Abdel Ghfar S.S., Khalil A.A. (2022 a). The single or combined Silybum marianum and co-enzyme Q10 role in alleviating fluoride-induced impaired growth, immune suppression, oxidative stress, histological alterations, and reduced resistance to Aeromonas sobria in African catfish (Clarias gariepinus). Aquaculture, 548: 737693.
- El-Houseiny W., Khalil A.A., Abd-Elhakim Y.M., Arisha A.H., Moselhy A.A.A., Dahshan H., Saber T., Saber T.M., Ahmed M.M. (2022 b). Alleviative effects of dietary Silybum marianum and co-enzyme Q10 on waterborne nickel-induced impaired growth, immunosuppression, tissue damage, immune-related genes dysregulation, and reduced resistance to Pseudomonas aeruginosa in Oreochromis niloticus. Aquacult. Rep., 26: 101308.
- Elnesr S.S., Elwan H.A.M., El Sabry M.I., Shehata A.M. (2023). The nutritional importance of milk thistle (Silybum marianum) and its beneficial influence on poultry. World’s Poult. Sci. J., 79: 751–768.
- Encarnação P. (2016). Functional feed additives in aquaculture feeds, In: Aquafeed Formulation, Nates S.F. (ed.). Academic Press, San Diego, pp. 217–237.
- Esmaeil N., Anaraki S.B., Gharagozloo M., Moayedi B. (2017). Silymarin impacts on immune system as an immunomodulator: One key for many locks. Int. Immunopharmacol., 50: 194–201.
- Fagnon M.S., Thorin C., Calvez S. (2020). Meta-analysis of dietary supplementation effect of turmeric and curcumin on growth performance in fish. Rev. Aquacult., 12: 2268–2283.
- Fanai S., Bakhshi D., Abbaszadeh B. (2024). Physiological and biochemical characteristics of milk thistle (Silybum marianum (L.) Gaertn) as affected by some plant growth regulators. Food Sci. Nutr., 12: 6022–6033.
- FAO (2024). The State of World Fisheries and Aquaculture 2024. Blue Transformation in Action. FAO Rome, Italy.
- Ferreira M., dos Santos P., dos Santos G. (2024). Azadirachta indica as a sustainable nutritional alternative in Nile tilapia farming – A mini review. Probe – Fish. Sci. Aquac., 6: 2208.
- Gazak R., Walterova D., Kren V. (2007). Silybin and silymarin – new and emerging applications in medicine. Curr. Med. Chem., 14: 315–338.
- Grădinariu L., Dediu L., Crețu M., Grecu I.R., Docan A., Istrati D.I., Dima F.M., Stroe M.D., Vizireanu C. (2024). The antioxidant and hepatoprotective potential of berberine and silymarin on acetaminophen induced toxicity in Cyprinus carpio L. Animals, 14: 373.
- Guerrini A., Tedesco D.E.A. (2023). Restoring activity of milk thistle (Silybum marianum L.) on serum biochemical parameters, oxidative status, immunity, and performance in poultry and other animal species, poisoned by mycotoxins: a review. Animals, 13: 330.
- Hasanthi M., Jo S., Kim H.-S., Yun K.-S., Lee Y., Lee K.-J. (2024). Dietary supplementation of micelle silymarin enhances the antioxidant status, innate immunity, growth performance, resistance against Vibrio parahaemolyticus infection, and gut morphology in Pacific white shrimp (Litopenaeus vannamei). Anim. Feed Sci. Technol., 311: 115953.
- Hashemi S.R., Davoodi H. (2011). Herbal plants and their derivatives as growth and health promoters in animal nutrition. Vet. Res. Commun., 35: 169–180.
- Hassaan M.S., Mohammady E.Y., Soaudy M.R., El-Garhy H.A.S., Moustafa M.M.A., Mohamed S.A., El-Haroun E.R. (2019). Effect of Silybum marianum seeds as a feed additive on growth performance, serum biochemical indices, antioxidant status, and gene expression of Nile tilapia, Oreochromis niloticus (L.) fingerlings. Aquaculture, 509: 178–187.
- Iqbal T., Salma U., Umair M., Iqbal H., Asmara, Khalid T., Hyder S. (2024). Utilizing medicinal plants for disease treatment in aquaculture: An approach to improve fish health: Medicinal Plants in Aquaculture. Markhor (J. Zool.). 5: 03–10.
- Ivanova S., Sukhikh S., Popov A., Shishko O., Nikonov I., Kapitonova E., Krol O., Larina V., Noskova S., Babich O. (2024). Medicinal plants: A source of phytobiotics for the feed additives. J. Agric. Food Res., 16: 101172.
- Jacobs B.P., Dennehy C., Ramirez G., Sapp J., Lawrence V.A. (2002). Milk thistle for the treatment of liver disease: A systematic review and meta-analysis. Am. J. Med., 113: 506–515.
- Jaffar H.M., Al-Asmari F., Khan F.A., Rahim M.A., Zongo E. (2024). Silymarin: Unveiling its pharmacological spectrum and therapeutic potential in liver diseases – A comprehensive narrative review. Food Sci. Nutr., 12: 3097–3111.
- Janči T., Putnik P. (2025). Sustainable fish production: Aquaculture systems and environmental impacts, In: Environmental remediation in agri-food industry using nanotechnology and sustainable strategies, Putnik P., Merkulov D.Š. (eds). Academic Press, pp. 7–22.
- Jia F., Wei L., Jiang W.-D., Wu P., Liu Y., Ma Y.-B., Jin X.-W., Ren H.-M., Zhou X.-Q., Feng L. (2025). New perspectives on the immune regulatory role of dietary silymarin in the intestines of juvenile grass carp (Ctenopharyngodon idella). Aquaculture, 602: 742336.
- Jindal R., Sharma R., Kaur P., Kaur S., Multisanti C.R., Faggio C. (2024). Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure. Heliyon, 10.
- Khalil A.A., Abd-Elhakim Y.M., Said E.N., Moselhy A.A.A., Abu-Elsaoud A.M., El-Houseiny W. (2022). Milk thistle and co-enzyme Q10 fortified diets lessen the nickel chloride-induced neurotoxic and neurobehavioral impairments in Oreochromis niloticus via regulating the oxidative stress response, acetylcholinesterase activity, and brain nickel content. Aquaculture, 553: 738102.
- Khazaei R., Seidavi A., Bouyeh M. (2022). A review on the mechanisms of the effect of silymarin in milk thistle (Silybum marianum) on some laboratory animals. Vet. Med. Sci., 8: 289–301.
- Kim H.-S., Jo S., Yun K.-S., Lee K.-J. (2023). Effects of dietary micelle silymarin on the growth performance, feed utilization and health of olive flounder (Paralichthys olivaceus). Aquac. Int., 31: 3419–3436.
- Kuebutornye F.K.A., Roy K., Folorunso E.A., Mraz J. (2024). Plant-based feed additives in Cyprinus carpio aquaculture. Rev. Aquacult., 16: 309–336.
- Li Z., Zhang X., Aweya J.J., Wang S., Hu Z., Li S., Wen X. (2019). Formulated diet alters gut microbiota compositions in marine fish Nibea coibor and Nibea diacanthus. Aquac. Res., 50: 126–138.
- Liang H., Tran N.T., Deng T., Li J., Lei Y., Bakky M.A.H., Zhang M., Li R., Chen W., Zhang Y., Chen X., Li S. (2023). Identification and characterization of a potential probiotic, Clostridium butyricum G13, isolated from the intestine of the mud crab (Scylla paramamosain). Microbiol. Spectr., 11: e01317–01323.
- López-Carvallo J.A., Mazón-Suástegui J.M., Arcos-Ortega G.F., Hernández-Oñate M.Á., Tovar-Ramírez D., Abasolo-Pacheco F., García-Bernal M. (2022). Highly diluted bioactive compounds in marine aquaculture: A potential alternative for sustainable production. Rev. Aquacult., 14: 1170–1193.
- Makkar H.P.S., Tran G., Heuzé V., Ankers P. (2014). State-of-the-art on use of insects as animal feed. Anim. Feed Sci. Technol., 197: 1–33.
- Marceddu R., Dinolfo L., Carrubba A., Sarno M., Di Miceli G. (2022). Milk thistle (Silybum Marianum L.) as a novel multipurpose crop for agriculture in marginal environments: a review. Agronomy, 12: 729.
- Marimuthu V., Sarawagi A.D., Kumar A., Paul S., Sampath V., Issara U., Al-Dhabi N.A., Arasu M.V., Balasubramanian B., Sureshkumar S. (2022). Glimpse of feed and feed additive necessity and mycotoxin challenges in aquaculture, In: Aquaculture Science and Engineering, Balasubramanian B., Liu W.-C., Sattanathan G. (eds). Springer Nature Singapore, Singapore, pp. 401–430.
- Mihailović V., Srećković N., Popović-Djordjević J.B. (2023). Silybin and silymarin: phytochemistry, bioactivity, and pharmacology, In: Handbook of Dietary Flavonoids, Xiao J. (ed.). Springer International Publishing, Cham, pp. 1–45.
- Muchiri R.N., van Breemen R.B. (2024). Chemical standardization of milk thistle (Silybum marianum L.) extract using UHPLC-MS/MS and the method of standard addition. J. Am. Soc. Mass Spectrom., 35: 1726–1732.
- Nazdar N., Imani A., Noori F., Sarvi Moghanlou K. (2018). Effect of silymarin supplementation on nickel oxide nanoparticle toxicity to rainbow trout (Oncorhynchus mykiss) fingerlings: pancreas tissue histopathology and alkaline protease activity. Iran. J. Sci. Technol., Trans. A: Sci., 42: 353–361.
- New Rules for Farming, Processing, and Organic Controls. Eur. Food Feed Law Rev., 15: 2–17.
- Okasha L.A., Tawfeek W.S., Elbaz S., Sherif A.H. (2025). Lactobacillus plantarum ameliorates stress caused by Pseudomonas aeruginosa infection in Nile tilapia. Res. Vet. Sci., 192: 105721.
- Onomu A.J., Okuthe G.E. (2024). The role of functional feed additives in enhancing aquaculture sustainability. Fishes, 9: 167.
- Owatari M.S., Alves Jesus G.F., Brum A., Pereira S.A., Lehmann N.B., de Pádua Pereira U., Martins M.L., Pedreira Mouriño J.L. (2018). Sylimarin as hepatic protector and immunomodulator in Nile tilapia during Streptococcus agalactiae infection. Fish Shellfish Immunol., 82: 565–572.
- Porwal O., Ameen M.M., Anwer E.T., Uthirapathy S., Ahamad J., Tahsin A. (2019). Silybum marianum (milk thistle): Review on its chemistry, morphology, ethno medical uses, phytochemistry and pharmacological activities. J. Drug Deliv. Ther., 9: 199–206.
- Presenza L., Ferraz Teixeira B., Antunes Galvão J., Maria Ferreira de Souza Vieira T. (2023). Technological strategies for the use of plant-derived compounds in the preservation of fish products. Food Chem., 419: 136069.
- Reverter M., Bontemps N., Lecchini D., Banaigs B., Sasal P. (2014). Use of plant extracts in fish aquaculture as an alternative to chemotherapy: Current status and future perspectives. Aquaculture, 433: 50–61.
- Samat N., Goh K.H., See K.F. (2024). Review of the application of cost–benefit analysis to the development of production systems in aquaculture. Aquaculture, 587: 740816.
- Schmidt H., Haccius M. (2020). Analysis of Regulation (EU) 2018/848 on Organic Food.
- Seidavi A., Hosseintabar-Ghasemabad B., Gorlov I.F., Slozhenkina M.I., Mosolov A.A., Oteri M., Chiofalo B., Liotta L. (2023). Feed additives and future perspectives, In: Sustainable Use of Feed Additives in Livestock: Novel Ways for Animal Production, Arsenos G., Giannenas I. (eds). Springer International Publishing, Cham, pp. 929–961.
- Shahin S.A., Mansour A.T., Abdel-Rahim M.M., El-Dahhar A.A., El Basuini M.F., Elhetawy A.I. (2023). Silymarin, Silybum marianum, supplemented weaning diet boosted survival, growth, antioxidant status, and fatty acids profile of seabass, Dicentrarchus labrax. Ann. Anim. Sci., 23: 253–264.
- Shahsavan M. (2025). Mode of action of milk thistle (Silybum marianum) derivations in poultry: intestine–liver axis, immunity, and woody breast muscle disorder. World’s Poult. Sci. J., 81: 227–253.
- Sharma R., Barange M., Agostini V., Barros P., Gutierrez N., Vasconcellos M., Fernandez Reguera D., Tiffay C., Levontin P. (2025). Review of the state of world marine fishery resources – 2025. FAO Fisheries and Aquaculture Technical Paper, No. 721, Rome, Italy.
- Shehata A.M., Abdel-Moneim A.-M.E., Gewida A.G., Abd El-Hack M.E., Alagawany M., Naiel M.A. (2022). Phytogenic Substances: A Promising Approach Towards Sustainable Aquaculture Industry, Antibiotic Alternatives in Poultry and Fish Feed, Bentham Science Publishers, pp. 160–193.
- Shehata A.I., Rasheed M., Rafiq H., Khalid N., Rafique A., Alhoshy M., Habib Y.J., El Basuini M.F. (2024 a). Multi-functional application of octacosanol as a feed additive in animal and aquaculture: A review. J. Anim. Physiol. Anim. Nutr., 108: 1595–1603.
- Shehata A.I., Shahin S.A., Elmaghraby A.M., Alhoshy M., Soliman A.A., Amer A.A., Habib Y.J., Gewaily M.S., El Basuini M.F. (2024 b). Synergistic benefits of dietary silymarin and selenium on growth, immune functions, antioxidants, and gut/liver health of thinlip mullet (Liza ramada) juveniles. Ann. Anim. Sci., 24: 1293–1309.
- Shehata A.I., Li S., Alhoshy M., Mabrouk H.A.H., Sallam G.R., Al-Absawey M.A., Teiba I.I., Mzengereza K., Fayed W.M., Habib Y.J., El Basuini M.F. (2025 a). The potential application of prebiotic Stevia rebaudiana in aquaculture and animal feed. Aquac. Nutr., 2025: 3858421.
- Shehata A.I., Tefal E., Elmaghraby A.M., Amer A.A., Teiba I.I., Alhoshy M., Gewaily M.S., Guo Z., Li S., El Basuini M.F. (2025 b). Evaluation of silymarin–L-carnitine as a dietary supplement on growth performance, antioxidants and immunity, gut/liver health, and gene expression in Nile tilapia (Oreochromis niloticus). Fishes, 10: 580.
- Sherif A.H., Toulan A.E., El-kalamwi N., Farag E.A.H., Mahmoud A.E. (2023). Silymarin enhances the response to oxytetracycline treatment in Oreochromis niloticus experimentally infected with Aeromonas hydrophila. Sci. Rep., 13: 16235.
- Song I.-S., Nam S.-J., Jeon J.-H., Park S.-J., Choi M.-K. (2021). Enhanced bioavailability and efficacy of silymarin solid dispersion in rats with acetaminophen-induced hepatotoxicity. Pharmaceutics, 13: 628.
- Sumana S.L., Xue T., Hu H., Abdullateef M.M., Shui Y., Ayana G.U., Kayiira J.C., Zhang C., Samwel B.J., Zhu J., Su S., Ebenezer A., Simbo E.B.S., Xiaojun J. (2025). Medicinal plants as ecological solutions for fish growth and immunostimulatory effects in aquaculture. Aquacult. Res., 2025: 9778623.
- Surai P.F. (2015). Silymarin as a natural antioxidant: an overview of the current evidence and perspectives. Antioxidants, 4: 204–247.
- Surai A., Surai P.F. (2023 a). Antitoxic properties of silymarin. Wageningen Academic.
- Surai P., Surai A. (2023 b). Silymarin and inflammation: From understanding molecular mechanisms to practical applications. Wageningen Academic Publishers: Wageningen, The Netherlands, pp. 287–317.
- Surai P.F., Surai A., Earle-Payne K. (2024). Silymarin and inflammation: food for thoughts. Antioxidants, 13: 98.
- Tacon A.G.J., Marc M., McNevin A.A. (2022). Future feeds: suggested guidelines for sustainable development. Rev. Fish. Sci. Aquac., 30: 135–142.
- Tawfeek W.S., Kassab A.S., Al-Sokary E.T., Abass M.E., Sherif A.H. (2024). Chlorella vulgaris algae ameliorates chlorpyrifos toxicity in Nile tilapia with special reference to antioxidant enzymes and Streptococcus agalactiae infection. Mol. Biol. Rep., 51: 616.
- Tedesco D.E.A., Guerrini A. (2023). Use of milk thistle in farm and companion animals: a review. Planta Med., 89: 584–607.
- Tran N.T., Li Z., Wang S., Zheng H., Aweya J.J., Wen X., Li S. (2020). Progress and perspectives of short-chain fatty acids in aquaculture. Rev. Aquacult., 12: 283–298.
- Tvrdý V., Pourová J., Jirkovský E., Křen V., Valentová K., Mladěnka P. (2021). Systematic review of pharmacokinetics and potential pharmacokinetic interactions of flavonolignans from silymarin. Med. Res. Rev., 41: 2195–2246.
- Valenzuela-Gutiérrez R., Lago-Lestón A., Vargas-Albores F., Cicala F., Martínez-Porchas M. (2021). Exploring the garlic (Allium sativum) properties for fish aquaculture. Fish Physiol. Biochem., 47: 1179–1198.
- Veisi S., Johari S.A., Tyler C.R., Mansouri B., Esmaeilbeigi M. (2021). Antioxidant properties of dietary supplements of free and nanoencapsulated silymarin and their ameliorative effects on silver nanoparticles induced oxidative stress in Nile tilapia (Oreochromis niloticus). Environ. Sci. Pollut. Res., 28: 26055–26063.
- Vijayaram S., Sun Y.-Z., Zuorro A., Ghafarifarsani H., Van Doan H., Hoseinifar S.H. (2022). Bioactive immunostimulants as health-promoting feed additives in aquaculture: A review. Fish Shellfish Immunol., 130: 294–308.
- Viktorová J., Dobiasová S., Řehořová K., Biedermann D., Káňová K., Šeborová K., Václavíková R., Valentová K., Ruml T., Křen V., Macek T. (2019). Antioxidant, anti-inflammatory, and multidrug resistance modulation activity of silychristin derivatives. Antioxidants, 8: 303.
- Wang J., Zhou H., Wang X., Mai K., He G. (2019). Effects of silymarin on growth performance, antioxidant capacity and immune response in turbot (Scophthalmus maximus L.). J. World Aquac. Soc., 50: 1168–1181.
- Wei L., Wu P., Zhou X.-Q., Jiang W.-D., Liu Y., Kuang S.-Y., Tang L., Feng L. (2020). Dietary silymarin supplementation enhanced growth performance and improved intestinal apical junctional complex on juvenile grass carp (Ctenopharyngodon idella). Aquaculture, 525: 735311.
- Woodgate S.L., Wan A.H.L., Hartnett F., Wilkinson R.G., Davies S.J. (2022). The utilisation of European processed animal proteins as safe, sustainable and circular ingredients for global aquafeeds. Rev. Aquacult., 14: 1572–1596.
- Xiao P., Ji H., Ye Y., Zhang B., Chen Y., Tian J., Liu P., Chen L., Du Z. (2017). Dietary silymarin supplementation promotes growth performance and improves lipid metabolism and health status in grass carp (Ctenopharyngodon idellus) fed diets with elevated lipid levels. Fish Physiol. Biochem., 43: 245–263.
- Xie Y., Zhang D., Zhang J., Yuan J. (2019). Metabolism, transport and drug–drug interactions of silymarin. Molecules, 24: 3693.
- Yang C., Chowdhury M.A.K., Huo Y., Gong J. (2015). Phytogenic compounds as alternatives to in-feed antibiotics: potentials and challenges in application. Pathogens, 4: 137–156.
- Yang Q., Lü Y., Zhang M., Gong Y., Li Z., Tran N.T., He Y., Zhu C., Lu Y., Zhang Y., Li S. (2019). Lactic acid bacteria, Enterococcus faecalis Y17 and Pediococcus pentosaceus G11, improved growth performance, and immunity of mud crab (Scylla paramamosain). Fish Shellfish Immunol., 93: 135–143.
- Zhang Z., Li X., Sang S., McClements D.J., Chen L., Long J., Jiao A., Wang J., Jin Z., Qiu C. (2022). A review of nanostructured delivery systems for the encapsulation, protection, and delivery of silymarin: An emerging nutraceutical. Food Res. Int., 156: 111314.
Language: English
Submitted on: Jul 15, 2025
Accepted on: Oct 16, 2025
Published on: Jun 5, 2026
Published by: National Research Institute of Animal Production
In partnership with: Paradigm Publishing Services
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© 2026 Akram Ismael Shehata, Shengkang Li, Yusuf Jibril Habib, Md. Akibul Hasan Bakky, Afaf N. Abdel Rahman, Mohammed F. El Basuini, published by National Research Institute of Animal Production
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