Figure 1.
The purity of endometrial epithelial cell culture confirmed by positive immunofluorescent staining for anti-cytokeratin (A) and negative anti-vimentin staining (B). Negative control without primary antibodies for cytokeratin (C) and vimentin (D). Scale bar: 20 μm.
Table 1.
Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) alone or antagonists of β-ARs (10−4 M) alone on the prostaglandin-endoperoxidase synthase-2 (PTGS-2), microsomal PTGE synthase-1 (mPTGES-1) and mPTGES-2 protein abundances in the pig cultured endometrial epithelial cells.
| Treatment | PTGS-2 | mPTGES-1 | mPTGES-2 |
|---|---|---|---|
| protein abundances (arbitrary units) | |||
| CV | 0.19±0.03 | 0.03±0.01 | 0.29±0.03a |
| NA | 0.18±0.08 | 0.04±0.02 | 0.54±0.04b |
| α1 agon. | 0.22±0.04 | 0.04±0.02 | 0.18±0.05a |
| α2 agon. | 0.16±0.05 | 0.03±0.01 | 0.21±0.02a |
| β agon. | 0.22±0.03 | 0.03±0.02 | 0.71±0.06b |
| β1 agon. | 0.23±0.02 | 0.07±0.03 | 0.58±0.02b |
| β2 agon. | 015±0.04 | 0.05±0.03 | 1.23±0.04c |
| β3 agon. | 0.25±0.03 | 0.03±0.01 | 0.59±0.03b |
| β1 anta. | 0.21±0.05 | 0.06±0.02 | 0.22±0.04a |
| β2 anta. | 0.15±0.03 | 0.04±0.01 | 0.25±0.05a |
| β3 anta. | 0.18±0.06 | 0.04±0.02 | 0.21±0.07a |
Figure 2.
Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the prostaglandin-endoperoxidase synthase-2 (PTGS-2) protein abundance in the lipopolysaccharide (LPS, 10 ng/ml of medium)-exposed pig cultured endometrial epithelial cells, estimated by Western blotting. Treatments were conducted in triplicate for each of the four pigs. The values were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) abundances. Data are presented as the mean (± SEM). Blots with representative bands for each treatment are given in Supplementary Figure 2A. Different letters (a, b, c) indicate statistical differences (P<0.05–0.001). CV: control value
Figure 3.
Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the microsomal prostaglandin E synthase-1 (mPTGES-1) protein abundance in the lipopolysaccharide (LPS, 10 ng/ml of medium)-exposed pig cultures endometrial epithelial cells, estimated by Western blotting. Treatments were conducted in triplicate for each of the four pigs. The values were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) abundances. Data are presented as the mean (± SEM). Blots with representative bands for each treatment are given in Supplementary Figure 2B. Different letters (a, b, c, d) indicate statistical differences (P<0.05–0.001). CV: control value
Figure 4.
Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the microsomal prostaglandin E synthase-2 (mPTGES-2) protein abundance in the lipopolysaccharide (LPS, 10 ng/ml of medium)-exposed pig cultured endometrial epithelial cells, estimated by Western blotting. Treatments were conducted in triplicate for each of the four pigs. The values were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) abundances. Data are presented as the mean (± SEM). Blots with representative bands for each treatment are given in Supplementary Figure 2C. Different letters (a, b) indicate statistical differences (P<0.05, P<0.01). CV: control value
Table 2.
Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) alone or antagonists of β-ARs (10−4 M) alone on the prostaglandin E2 (PGE2) content in the medium of pig cultured endometrial epithelial cells.
Figure 5.
Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the prostaglandin E2 (PGE2) concentration in medium after incubation of the lipopolysaccharide (LPS, 10 ng/ml medium)-exposed pig cultured endometrial epithelial cells, estimated by ELISA. Treatments were conducted in triplicate for each of the four pigs. Data are presented as the mean (± SEM). Different letters (a, b, c) indicate statistical differences (P<0.001). CV: control value