Role of noradrenaline and adrenoreceptors in prostaglandin E2 generation in Escherichia coli lipopolysaccharide-treated porcine endometrial epithelial cells Cover

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Role of noradrenaline and adrenoreceptors in prostaglandin E2 generation in Escherichia coli lipopolysaccharide-treated porcine endometrial epithelial cells

Annals of Animal Science

By: Barbara Jana, Aleksandra Mówińska, Aneta Andronowska and Jarosław Całka

Open Access

|Feb 2026

Figures & Tables

The purity of endometrial epithelial cell culture confirmed by positive immunofluorescent staining for anti-cytokeratin (A) and negative anti-vimentin staining (B). Negative control without primary antibodies for cytokeratin (C) and vimentin (D). Scale bar: 20 μm.

Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the prostaglandin-endoperoxidase synthase-2 (PTGS-2) protein abundance in the lipopolysaccharide (LPS, 10 ng/ml of medium)-exposed pig cultured endometrial epithelial cells, estimated by Western blotting. Treatments were conducted in triplicate for each of the four pigs. The values were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) abundances. Data are presented as the mean (± SEM). Blots with representative bands for each treatment are given in Supplementary Figure 2A. Different letters (a, b, c) indicate statistical differences (P<0.05–0.001). CV: control value

Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the microsomal prostaglandin E synthase-1 (mPTGES-1) protein abundance in the lipopolysaccharide (LPS, 10 ng/ml of medium)-exposed pig cultures endometrial epithelial cells, estimated by Western blotting. Treatments were conducted in triplicate for each of the four pigs. The values were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) abundances. Data are presented as the mean (± SEM). Blots with representative bands for each treatment are given in Supplementary Figure 2B. Different letters (a, b, c, d) indicate statistical differences (P<0.05–0.001). CV: control value

Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the microsomal prostaglandin E synthase-2 (mPTGES-2) protein abundance in the lipopolysaccharide (LPS, 10 ng/ml of medium)-exposed pig cultured endometrial epithelial cells, estimated by Western blotting. Treatments were conducted in triplicate for each of the four pigs. The values were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) abundances. Data are presented as the mean (± SEM). Blots with representative bands for each treatment are given in Supplementary Figure 2C. Different letters (a, b) indicate statistical differences (P<0.05, P<0.01). CV: control value

Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) or antagonists of β1-, β2- and β3-ARs (10−4 M) with NA or antagonists of β1-, β2- and β3-ARs (10−4 M) in combination with agonists of β1-, β2-, β3-ARs (10−4 M) on the prostaglandin E2 (PGE2) concentration in medium after incubation of the lipopolysaccharide (LPS, 10 ng/ml medium)-exposed pig cultured endometrial epithelial cells, estimated by ELISA. Treatments were conducted in triplicate for each of the four pigs. Data are presented as the mean (± SEM). Different letters (a, b, c) indicate statistical differences (P<0.001). CV: control value

Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) alone or antagonists of β-ARs (10−4 M) alone on the prostaglandin-endoperoxidase synthase-2 (PTGS-2), microsomal PTGE synthase-1 (mPTGES-1) and mPTGES-2 protein abundances in the pig cultured endometrial epithelial cells_

Treatment	PTGS-2	mPTGES-1	mPTGES-2
Treatment	protein abundances (arbitrary units)
CV	0.19±0.03	0.03±0.01	0.29±0.03^a
NA	0.18±0.08	0.04±0.02	0.54±0.04^b

α1 agon.	0.22±0.04	0.04±0.02	0.18±0.05^a
α2 agon.	0.16±0.05	0.03±0.01	0.21±0.02^a
β agon.	0.22±0.03	0.03±0.02	0.71±0.06^b

β1 agon.	0.23±0.02	0.07±0.03	0.58±0.02^b
β2 agon.	015±0.04	0.05±0.03	1.23±0.04^c
β3 agon.	0.25±0.03	0.03±0.01	0.59±0.03^b

β1 anta.	0.21±0.05	0.06±0.02	0.22±0.04^a
β2 anta.	0.15±0.03	0.04±0.01	0.25±0.05^a
β3 anta.	0.18±0.06	0.04±0.02	0.21±0.07^a

Influence of noradrenaline (NA, 10−5 M) alone or agonists of α1-, α2- and β-adrenoreceptors (ARs, 10−4 M) alone or antagonists of β-ARs (10−4 M) alone on the prostaglandin E2 (PGE2) content in the medium of pig cultured endometrial epithelial cells_

Treatment	PGE2 content (ng/ml of medium)
CV	0.71±0.15^a
NA	1.52±0.13^b

α1 agon.	0.59±0.17^a
α2 agon.	0.68±0.14^a
β agon.	1.91±0.15^cb

β1 agon.	2.38±0.09^cd
β2 agon.	2.74±0.11^d
β3 agon.	2.65±0.13^d

β1 anta.	0.62±0.14^a
β2 anta.	0.53±0.09^a
β3 anta.	0.83±0.14^a

DOI: https://doi.org/10.2478/aoas-2025-0115 | Journal eISSN: 2300-8733 | Journal ISSN: 1642-3402

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Language: English

Submitted on: Feb 4, 2025

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Accepted on: Oct 9, 2025

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Published on: Feb 17, 2026

Published by: National Research Institute of Animal Production

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

endometrial epithelial cells,

lipopolysaccharide,

adrenoreceptors,

prostaglandin E2

Related subjects:

Veterinary medicine

© 2026 Barbara Jana, Aleksandra Mówińska, Aneta Andronowska, Jarosław Całka, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 3.0 License.