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The Importance of Flocculating Agents in Biofloc Aquaculture System: New Flocculants and Their Performance on Water Quality, Sludge Production, Heavy Metals, and Microplastic Cover

The Importance of Flocculating Agents in Biofloc Aquaculture System: New Flocculants and Their Performance on Water Quality, Sludge Production, Heavy Metals, and Microplastic

Open Access
|Jan 2026

Figures & Tables

Figure 1.

Properties of chitosan as flocculating agent
Properties of chitosan as flocculating agent

Bioflocculants used in wastewater treatment at various concentrations and having potential to be used in BFT

Source of flocculantsEffective concentrationReferences
Abelmoschus esculentus80 mg/LCarneiro-Marra et al. (2019)
Banana juice90 mLGautam and Saini (2020)
Bitter gourd seed400 mg/LBabarao and Verma (2015)
Cactus0.4 g/LDeshmukh and Hedaoo (2018)
Castor oil cake40 mLSheikh et al. (2016)
Cicer arietinum0.1 g/LFerrari et al. (2016)
Cicer arietinum68 mg/LLaksmi et al. (2017)
Cotton seed oil cake40 g/LNarmatha et al. (2017)
Dolichos lablab450 mg/LDaverey et al. (2019); Laksmi et al. (2017)
Hibiscus rosa sinensi500 mg/LAwang and Aziz (2012)
Malva sylvestris (mallow)12 mg/LAnastasakis et al. (2009)
Moringa oleifera1 g/L; 9.4 mL/LChonde and Raut (2017); de Paula et al. (2018)
Ocimum basilicum1.6 mg/LShamsnejati et al. (2015)
Opuntia mucilage21.1 mg/LCarpinteyro-Urban et al. (2012)
Plantago major L.297.6 mg/LGautam and Saini (2020)
Plantago ovata1.5 mg/LRamavandi and Farjadfard (2016); Mishra and Bajpai (2005)
Psyllium husk0.4 g/L and 7.2 g/LGautam and Saini (2020)
Sechium edule (chayote)15 mg/LAlmeida et al. (2017)
Strychno spotatorum60 mLDehghani and Alizadeh (2016)
Surjana seed30 g/LPatel and Vashi (2013)
Tamarind indica1250 mg/LLaksmi et al. (2017)
Tanfloc SG20 mg/LGautam and Saini (2020)
Trigonella foenum-graecus0.1 g/LPatil and Hugar (2015)
Zea mays30 g/LPatel and Vashi (2013)

Chemical coagulants utilized for wastewater treatment

CoagulantsChemical formulaConcentrationReferences
Aluminium sulphateAl2(SO4)37.2 mL/L and 40 mg/LAkinwole et al. (2016); Dehghani and Alizadeh (2016); de Paula et al. (2018)
Ferric chlorideFeCl33.6 mL/LSarparastzadeh et al. (2007); de Paula et al. (2018)
Ferric sulphateFe2(SO4)3400 mg/LBabarao and Verma (2015)
Iron (III) chloride hexahydrateCl3FeH12O6500 mg/LGautam and Saini (2020)
LimeCaO600 mg/LLin et al. (2017)
Magnesium chlorideMgCl3120 mg/LVerma et al. (2012)
Poly aluminium chloride (PAC) 500 mg/LGautam and Saini (2020)
Poly aluminium ferric chloride (PAFC) 3 mg/LGkotsis et al. (2017)
Poly aluminium silicate chloride (PASic) 9 mg/LLiao and Zhang (2018)
Poly ferric acetate 24 mg/LWei et al. (2017)
Poly ferric sulphate 20 mg/LWei et al. (2017)
Potassium aluminium sulphate KAl(SO4)2×12H2O0.25 g/LMalik (2018)

A comparative study of potential flocculant types for use in BFT

TypeExampleCharacteristicsReference
InorganicAluminium sulphate, ferric chloride, ferric sulphateEffective, inexpensive, corrosive, can leave behind residual chemicalsAgunbiade et al. (2016)
OrganicPolyacrylamide, polyacrylic acidLess corrosive than inorganic flocculants, do not leave behind residual chemicals, can be more expensiveXia et al. (2017)
SpecialtyDesigned for specific applications, such as wastewater treatment or miningCan be more effective or more cost-effective than general-purpose flocculantsKurniawan et al. (2023)
DOI: https://doi.org/10.2478/aoas-2025-0069 | Journal eISSN: 2300-8733 | Journal ISSN: 1642-3402
Language: English
Page range: 3 - 13
Submitted on: Jan 6, 2025
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Accepted on: Jun 5, 2025
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Published on: Jan 30, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2026 Soibam Khogen Singh, Maibam Malemngamba Meitei, Debojit Dekari, Surajkumar Irungbam, Supratim Malla, Nayan Chouhan, Lokesh Pawar, Dharmendra Kumar Meena, Mohammad Hossein Khanjani, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 3.0 License.