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Figure 6.
The identified overrepresented GO terms and KEGG pathways based on DEGs modified during differentiation of MSCs to ADMs
| Accession number | Number of DEGs* | FDR | |
|---|---|---|---|
| Biological process | |||
| regulation of signalling | GO:0023051 | 393 | 0.00001 |
| fat cell differentiation | GO:0045444 | 47 | 0.0001 |
| positive regulation of cell differentiation | GO:0045597 | 125 | 0.001 |
| regulation of cell differentiation | GO:0045595 | 207 | 0.001 |
| regulation of cell communication | GO:0010646 | 390 | 0.001 |
| negative regulation of apoptotic process | GO:0043066 | 40 | 0.01 |
| response to growth factor | GO:0070848 | 88 | 0.01 |
| positive regulation of metabolic process | GO:0009893 | 370 | 0.02 |
| cell development | GO:0048468 | 241 | 0.03 |
| KEGG pathway | |||
| biosynthesis of amino acids | ecb01230 | 21 | 0.001 |
| metabolic pathways | ecb01100 | 200 | 0.01 |
| AGE-RAGE signalling pathway in diabetic complications | ecb04933 | 24 | 0.02 |
| aldosterone-regulated sodium reabsorption | ecb04960 | 13 | 0.02 |
| HIF-1 signalling pathway | ecb04066 | 25 | 0.02 |
| MAPK signalling pathway | ecb04010 | 50 | 0.02 |
| cAMP signalling pathway | ecb04024 | 40 | 0.02 |
The identified overrepresented GO terms and KEGG pathways based on DEGs modified during differentiation of MSCs to CDMs
| Accession number | Number of DEGs* | FDR | |
|---|---|---|---|
| Biological process | |||
| regulation of cell differentiation | GO:0045595 | 96 | 0.00006 |
| ossification | GO:0001503 | 35 | 0.00006 |
| negative regulation of developmental process | GO:0051093 | 62 | 0.00007 |
| regulation of signalling | GO:0023051 | 159 | 0.0001 |
| regulation of cell communication | GO:0010646 | 158 | 0.0001 |
| response to growth factor | GO:0070848 | 48 | 0.0004 |
| regulation of cell proliferation | GO:0042127 | 82 | 0.0005 |
| cell adhesion | GO:0007155 | 16 | 0.0006 |
| regulation of cell death | GO:0010941 | 79 | 0.001 |
| apoptotic process | GO:0006915 | 79 | 0.003 |
| KEGG pathway | |||
| cGMP-PKG signalling pathway | ecb04022 | 17 | 0.05 |
| fluid shear stress and atherosclerosis | ecb05418 | 15 | 0.05 |
The Pearson correlation coefficients obtained during RNA-seq result validation using qPCR
| Gene | Pearson correlation coefficient | P value |
|---|---|---|
| MSC vs. CDM comparison | ||
| BMP7 | 0.99 | <0.0001 |
| BMPR1B | 0.89 | 0.021 |
| CLDN1 | 0.99 | <0.0001 |
| IHH | 0.99 | <0.0001 |
| SOX9 | 0.95 | 0.003 |
| PHOSPHO1 | 0.99 | <0.0001 |
| MSC vs. ADM comparison | ||
| LEP | 0.75 | 0.084 |
| SOX9 | 0.98 | 0.008 |
| FABP4 | 0.99 | 0.001 |
| GERP1 | 0.66 | 0.153 |