Have a personal or library account? Click to login
Spatial Dynamics of Cuprizone-Induced Myelin Loss and Recovery in the Mouse Corpus Callosum Cover

Spatial Dynamics of Cuprizone-Induced Myelin Loss and Recovery in the Mouse Corpus Callosum

Open Access
|Feb 2026

References

  1. Gean-Marton AD, Vezina LG, Marton KI, et al. Abnormal corpus callosum: a sensitive and specific indicator of multiple sclerosis. Radiology, 1991, 180(1):215-21. doi: 10.1148/radiology.180.1.2052698.
  2. Seitaridou Y, Dimitrova M, Chamova T, et al. Cost-effectiveness of multiple sclerosis therapies – a literature review. Acta Med Bulg, 2022, 49(4):69-80. doi: 10.2478/amb-2022-0046.
  3. Lindner M, Heine S, Haastert K, et al. Sequential myelin protein expression during remyelination reveals fast and enjcient repair after central nervous system demyelination. Neuro-pathol Appl Neurobiol, 2008, 34:105–114. doi: 10.1111/j.1365-2990.2007.00879.x.
  4. Matsushima GK, Morell P. The neurotoxicant, cuprizone, as a model to study demyelination and remyelination in the central nervous system. Brain Pathol, 2001, 11:107–116. doi: 10.1111/j.1750-3639.2001.tb00385.x.
  5. Kipp M. How to use the cuprizone model to study de- and remyelination. Int J Mol Sci, 2024, 25:1445. doi: 10.3390/ijms25031445.
  6. Hiremath MM, Saito Y, Knapp GW, et al. Microglial/macro-phage accumulation during cuprizone-induced demyelination in C57BL/6 mice. J Neuroimmunol, 1998, 1:38–49. doi: 10.1016/S0165-5728(98)00168-4.
  7. Gharagozloo M, Mace JW, Calabresi PA. Animal models to investigate the effects of inflammation on remyelination in multiple sclerosis. Front Mol Neurosci, 2022, 15:995477. doi: 10.3389/fnmol.2022.995477.
  8. Morgan ML, Teo W, Hernandez Y, et al. Cuprizone-induced Demyelination in Mouse Brain is not due to Depletion of Copper. ASN Neuro, 2022, 14:17590914221126367. doi: 10.1177/17590914221126367.
  9. Landzhov B, Gaydarski L, Stanchev S, et al. A Morphological and behavioral study of demyelination and remyelination in the cuprizone model: Insights into APLNR and NG2+ cell dynamics. Int J Mol Sci, 2024, 25(23):13011. doi: 10.3390/ijms252313011.
  10. Buonvicino D, Ranieri G, Chiarugi A. Cuprizone-dependent De/Remyelination responses and functional correlates in mouse strains adopted to model relapsing, chronic and progressive experimental autoimmune encephalomyelitis. Neurotox Res, 2021, 39(3):658-666. doi: 10.1007/s12640-021-00331-3.
  11. Lindner M, Fokuhl J, Linsmeier F, et al. Chronic toxic demyelination in the central nervous system leads to axonal damage despite remyelination. Neurosci Lett, 2009, 453(2):120-5. doi: 10.1016/j.neulet.2009.02.004.
  12. Paxinos G, Franklin K. The mouse brain in stereotaxic co-ordinates. 2nd ed. Academic Press. Cambridge, MA, USA, 2001, 55–63.
  13. Toomey LM, Papini M, Lins B, et al. Cuprizone feed formulation influences the extent of demyelinating disease pathology. Sci Rep, 2021, 11(1):22594. doi: 10.1038/s41598-021-01963-3.
  14. Hibbits N, Pannu R, Wu TJ, Armstrong RC. Cuprizone de-myelination of the corpus callosum in mice correlates with altered social interaction and impaired bilateral sensorimotor coordination. ASN Neuro, 2009, 1(3):e00013. doi: 10.1042/AN20090032.
  15. Steelman AJ, Thompson JP, Li J. Demyelination and remy-elination in anatomically distinct regions of the corpus callosum following cuprizone intoxication. Neurosci Res, 2012, 72(1):32-42. doi: 10.1016/j.neures.2011.10.002.
  16. Schmidt T, Awad H, Slowik A, et al. Regional heterogeneity of cuprizone-induced demyelination: topographical aspects of the midline of the corpus callosum. J Mol Neurosci, 2013, 49(1):80-8. doi: 10.1007/s12031-012-9896-0.
  17. Tagge I, O’Connor A, Chaudhary P, et al. Spatio-temporal patterns of demyelination and remyelination in the cupri-zone mouse model. PLoS One, 2016, 11(4):e0152480. doi: 10.1371/journal.pone.0152480.
  18. Fjær S, Bø L, Lundervold A, et al. Deep gray matter demyelination detected by magnetization transfer ratio in the cuprizone model. PLoS One, 2013, 8(12):e84162. doi: 10.1371/journal. pone.0084162. Erratum in: PLoS One. 2014;9(10):e111828.
  19. Yu Q, Hui R, Park J, et al. Strain differences in cuprizone induced demyelination. Cell Biosci, 2017, 7:59. doi: 10.1186/s13578-017-0181-3.
  20. Savaskan NE, Weinmann O, Heimrich B, Eyupoglu IY. High resolution neurochemical gold staining method for myelin in peripheral and central nervous system at the light- and electron-microscopic level. Cell Tissue Res, 2009, 337(2):213-21. doi: 10.1007/s00441-009-0815-9.
  21. Emery B. Regulation of oligodendrocyte differentiation and myelination. Science, 2010, 330(6005):779-82. doi: 10.1126/science.1190927.
  22. Wergeland S, Torkildsen Ø, Myhr KM, Mørk SJ, Bø L. The cuprizone model: regional heterogeneity of pathology. APMIS. 2012 Aug;120(8):648-57. doi: 10.1111/j.1600-0463.2012.02882.x.
DOI: https://doi.org/10.2478/amb-2026-0007 | Journal eISSN: 2719-5384 | Journal ISSN: 0324-1750
Language: English
Page range: 52 - 57
Submitted on: Jan 11, 2026
|
Accepted on: Jan 19, 2026
|
Published on: Feb 21, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 L. Gaydarski, K. Petrova, G.P. Georgiev, I. Kostadinova, published by Medical University - Sofia
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.