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Mechano-Responsive Polyampholyte Hydrogels with Strain-Stiffening Property for Wound Closure Applications Cover

Mechano-Responsive Polyampholyte Hydrogels with Strain-Stiffening Property for Wound Closure Applications

Open Access
|Sep 2025

Figures & Tables

Scheme 1.

Schematic Diagram Illustration (a) Preparation of TSC Polyampholyte hydrogel (b) Preparation of TSC-SS Polyampholyte under mechanical force (uniaxial stress)
Schematic Diagram Illustration (a) Preparation of TSC Polyampholyte hydrogel (b) Preparation of TSC-SS Polyampholyte under mechanical force (uniaxial stress)

Fig. 1.

Scanning electron microscopy (SEM) images of TSC polyampholyte hydrogel (a) before and (b) after self-assembly process
Scanning electron microscopy (SEM) images of TSC polyampholyte hydrogel (a) before and (b) after self-assembly process

Fig. 2.

Characterization of TSC polyampholyte (a) IR spectra of the TSC Polyampholyte the representative of the IR absorbance spectra of the TSC Polyampholyte (b) The amide-I band region (~1600-1700 cm−1)
Characterization of TSC polyampholyte (a) IR spectra of the TSC Polyampholyte the representative of the IR absorbance spectra of the TSC Polyampholyte (b) The amide-I band region (~1600-1700 cm−1)

Fig. 3.

Spectra of protein secondary structure analysis (a) the IR absorbance spectra's second derivatives contained to the amide-I band (b) IR spectra in the amide-I were fitted by approximating the number and position, which experimental curve (▪▪▪) and simulated fits (---) (c) Result simulated fits are the six Gaussian band prof.
Spectra of protein secondary structure analysis (a) the IR absorbance spectra's second derivatives contained to the amide-I band (b) IR spectra in the amide-I were fitted by approximating the number and position, which experimental curve (▪▪▪) and simulated fits (---) (c) Result simulated fits are the six Gaussian band prof.

Fig. 4.

Strain-stiffening curves of TSC polyampholyte
Strain-stiffening curves of TSC polyampholyte

Fig. 5.

The cell viability of HDF-a of the different culture plate (control), TSC-Non-SS polyampholyte and TSC-SS polyampholyte
The cell viability of HDF-a of the different culture plate (control), TSC-Non-SS polyampholyte and TSC-SS polyampholyte

Fig. 6.

The HDF-a cells spreading on strain-stiffening of TSC polyampholyte. Scale bar 25 μm
The HDF-a cells spreading on strain-stiffening of TSC polyampholyte. Scale bar 25 μm

Fig. 7.

In vitro cell culture, strain-stiffening polyampholyte (TSC-SS), nonstrain-stiffening polyampholyte (TSC-Non-SS) and control induce motility in HDF-a cells after 1 day. Scale bar = 200 μm
In vitro cell culture, strain-stiffening polyampholyte (TSC-SS), nonstrain-stiffening polyampholyte (TSC-Non-SS) and control induce motility in HDF-a cells after 1 day. Scale bar = 200 μm

Fig. 8.

Cell migration quantification of TSC polyampholyte
Cell migration quantification of TSC polyampholyte

Amide-I number and position (cm−1) of TSC polyampholyte

TSC polyampholyteAmide-I number and position (cm−1)
123456
T1S1C0~1601 (Side chain)~1602 (Side chain)~1617 (β-sheet)~1633 (β-sheet)~1651 (α-helix)~1664 (β-turn)
T1S0.5C0.5~1602 (Side chain)~1611 (β-sheet)~1623 (β-sheet)~1635 (β-sheet)~1656 (α-helix)~1675 (β-turn)
T1S0C1~1602 (Side chain)~1619 (Side chain)~1631 (β-sheet)~1650 (α-helix)~1665 (β-turn)~1680 (β-turn)
DOI: https://doi.org/10.2478/ama-2025-0051 | Journal eISSN: 2300-5319 | Journal ISSN: 1898-4088
Language: English
Page range: 441 - 446
Submitted on: Oct 2, 2024
Accepted on: Jun 16, 2025
Published on: Sep 30, 2025
Published by: Bialystok University of Technology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Gustini GUSTINI, Kaprawi SAHIM, Ida SRIYANTI, Irmawan IRMAWAN, published by Bialystok University of Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.