Table 1
Characteristics of Psychrobacter sanguinis strains isolated in the District Hospital in Limanowa, named after Divine Mercy.
Fig. 1.
Microscopic morphology of P. sanguinis (Gram stain, x1000) (ECLIPSE E200 optical microscope, Nikon Instruments Inc., Melville, NY, USA).
Fig. 2.
Morphology of Psychrobacter sanguinis colonies on Colombia agar with 5% horse blood (Oxoid Ltd., Hampshire, United Kingdom) (A) and MacConkey agar (Oxoid Ltd., Hampshire, United Kingdom) (B) after incubation by 18±2 hours in aerobic condition at 35°C.
Table 2
BLAST results for consensus sequences obtained in this study from Psychrobacter sanguinis st rains.
| Strain | Sequence | Genotypic species identification | 16S rRNA gene seq. Similarity (%) | Closest relative reference strain in BLAST |
|---|---|---|---|---|
| PS12844 | Psychrobacter sanguinis strain JM50 16S ribosomal RNA gene, partial sequence | P. sanguinis | 100.00% | MN758810.1 |
| PS12844 | Psychrobacter sanguinis strain N8K9ELMT 16S ribosomal RNA gene, partial sequence | P. sanguinis | 100.00% | MH178035.1 |
| PS12844 | Psychrobacter sanguinis strain CNM637-12 16S ribosomal RNA gene, partial sequence | P. sanguinis | 100.00% | KR232929.1 |
| PS1051 | Psychrobacter sanguinis strain JM50 16S ribosomal RNA gene, partial sequence | P. sanguinis | 99.71% | MN758810.1 |
| PS1051 | Psychrobacter sanguinis strain N8K9ELMT 16S ribosomal RNA gene, partial sequence | P. sanguinis | 99.71% | MH178035.1 |
Table 3
Comparison of MIC values of six antimicrobial agents for PS12844 and PS1051 strains with the corresponding MIC values proposed by EUCAST for aerobic Gram-negative bacteria lacking breakpoints in standard EUCAST breakpoint tables to assess the microbiological activity of the agent against the species.
| Antimicrobial agent | MIC (PS12844) | MIC (PS1051) | MIC – values above which therapy with the agent should be discouraged |
|---|---|---|---|
| Ciprofloxacin | 0.032 | 0.032 | 0.25 |
| Levofloxacin | 0.064 | 0.064 | 0.5 |
| Cefotaxime | 0.016 | 0.016 | 0.5 |
| Ceftazidime | 0.064 | 0.023 | 4 |
| Imipenem | 0.006 | 0.004 | 2 |
| Meropenem | 0.002 | 0.002 | 2 |