Fig. 1.
Fig. 2.
BLAST results for consensus sequences obtained in this study from Psychrobacter sanguinis st rains_
| Strain | Sequence | Genotypic species identification | 16S rRNA gene seq. Similarity (%) | Closest relative reference strain in BLAST |
|---|---|---|---|---|
| PS12844 | Psychrobacter sanguinis strain JM50 16S ribosomal RNA gene, partial sequence | P. sanguinis | 100.00% | MN758810.1 |
| PS12844 | Psychrobacter sanguinis strain N8K9ELMT 16S ribosomal RNA gene, partial sequence | P. sanguinis | 100.00% | MH178035.1 |
| PS12844 | Psychrobacter sanguinis strain CNM637-12 16S ribosomal RNA gene, partial sequence | P. sanguinis | 100.00% | KR232929.1 |
| PS1051 | Psychrobacter sanguinis strain JM50 16S ribosomal RNA gene, partial sequence | P. sanguinis | 99.71% | MN758810.1 |
| PS1051 | Psychrobacter sanguinis strain N8K9ELMT 16S ribosomal RNA gene, partial sequence | P. sanguinis | 99.71% | MH178035.1 |
Comparison of MIC values of six antimicrobial agents for PS12844 and PS1051 strains with the corresponding MIC values proposed by EUCAST for aerobic Gram-negative bacteria lacking breakpoints in standard EUCAST breakpoint tables to assess the microbiological activity of the agent against the species_
| Antimicrobial agent | MIC (PS12844) | MIC (PS1051) | MIC – values above which therapy with the agent should be discouraged |
|---|---|---|---|
| Ciprofloxacin | 0.032 | 0.032 | 0.25 |
| Levofloxacin | 0.064 | 0.064 | 0.5 |
| Cefotaxime | 0.016 | 0.016 | 0.5 |
| Ceftazidime | 0.064 | 0.023 | 4 |
| Imipenem | 0.006 | 0.004 | 2 |
| Meropenem | 0.002 | 0.002 | 2 |
Characteristics of Psychrobacter sanguinis strains isolated in the District Hospital in Limanowa, named after Divine Mercy_
| Strain No. | Date of isolation | Site of isolation | Unit | Sex/Age |
|---|---|---|---|---|
| PS12844 | 21.11.2023 | blood | ICUa | M/39 |
| PS1501 | 28.01.2024 | blood | IMUa | M/72 |