Change of minimum inhibitory concentration (MIC) values for fluconazole among the tested strains of which MIC changed after exposure to methotrexate (MTX) or pemetrexed (PMX)_ ‘The p-value in the first column reflects the outcome of the one-sided Wilcoxon test for paired samples_
| Change of minimal inhibitory concentration to Fluconazole [μg/mL] after stimulation with an antifolate | |||||
|---|---|---|---|---|---|
| All strains that showed an increase in MIC | C. albicans strains that showed an increase in MIC | All strains that showed a decrease in MIC | C. albicans strains that showed a decrease in MIC | ||
| After MTX | Minimal MIC change [%] | 25% | 25% | -50% | -50% |
| Maximal MIC change [%] | 1500% | 1100% | -63% | -50% | |
| Average MIC change [%] | 529% | 367% | -53% | -50% | |
| Number of strains | 7 | 6 | 5 | 3 | |
| p value | <0.01 | 0.02 | 0.03 | 0.13* | |
| After PMX | Minimal MIC change [%] | 50% | 50% | -25% | -25% |
| Maximal MIC change [%] | 700% | 700% | -75% | -50% | |
| Average MIC change [%] | 264% | 210% | -50% | -46% | |
| Number of strains | 7 | 5 | 10 | 7 | |
| p value | <0.01 | 0.03 | <0.01 | <0.01 | |
Summary characteristics of minimum inhibitory concentration (MIC) values for fluconazole among the tested strains before and after exposure to methotrexate (MTX) or pemetrexed (PMX)_ The p-value in the first column reflects the outcome of the one-sided Wilcoxon test for paired samples_
| Minimal inhibitory concentration to Fluconazole [μg/mL] | |||||
|---|---|---|---|---|---|
| All strains | C. albicans | C. tropicalis | N. glabratus | ||
| Before stimulation | Range | 0.13 – 16.00 | 0.13 – 1.00 | 0.25 – 1.00 | 1.00 – 16.00 |
| Median | 0.25 | 0.25 | 0.50 | 1.00 | |
| Geometric mean MIC | 0.40 | 0.24 | 0.50 | 2.52 | |
| After MTX | Range | 0.13 – 16.00 | 0.13 – 12.00 | 0.25 – 1.00 | 0.38 – 16.00 |
| Median | 0.28 | 0.25 | 0.25 | 16.00 | |
| Geometric mean MIC | 0.55 | 0.35 | 0.40 | 4.58 | |
| Average MIC change [%] | 191% | 171% | -17% | 479% | |
| Maximal MIC change [%] | 1500% | 1100% | -50% | 1500% | |
| p value | 0.75 | 0.86 | 0.16* | 0.67* | |
| After PMX | Range | 0.13 – 16.00 | 0.13 – 8.00 | 0.13 – 4.00 | 0.25 – 16.00 |
| Median | 0.19 | 0.16 | 0.25 | 6.00 | |
| Geometric mean MIC | 0.41 | 0.24 | 0.50 | 2.88 | |
| Average MIC change [%] | 75% | 61% | 67% | 142% | |
| Maximal MIC change [%] | 700% | 700% | 300% | 500% | |
| p value | 0.48 | 0.44 | 0.50* | 0.67* | |
Change in the minimum inhibitory concentration (MIC) value for Fluconazole before and after exposure to methotrexate (MTX) or pemetrexed (PMX) for each of the tested strains_ The color of the cell reflects changes in MIC for FLU after antifolate stimulation: red indicates an increase, green a decrease and white-no MIC change_
| Species | Strain number | MIC before stimulation [μg/mL] | MIC after MTX exposure (% of MIC change) | MIC after PMX exposure (% of MIC change) |
|---|---|---|---|---|
| C. albicans | 1 | 0.13 | 0.13 (0%) | 0.19 (50%) |
| 2 | 0.25 | 0.31 (25%) | 0.13 (-50%) | |
| 3 | 0.25 | 0.13 (-50%) | 0.13 (-50%) | |
| 4 | 1.00 | 12.00 (1100%) | 8.00 (700%) | |
| 5 | 0.25 | 0.13 (-50%) | 0.13 (-50%) | |
| 6 | 0.25 | 0.13 (-50%) | 0.13 (-50%) | |
| 7 | 0.25 | 0.25 (0%) | 0.19 (-25%) | |
| 8 | 0.25 | 0.25 (0%) | 0.13 (-50%) | |
| 9 | 0.25 | 2.25 (800%) | 0.50 (100%) | |
| 10 | 0.25 | 0.31 (25%) | 0.13 (-50%) | |
| 11 | 0.13 | 0.25 (100%) | 0.19 (50%) | |
| 12 * | 0.13 | 0.31 (150%) | 0.31 (150%) | |
| C. tropicalis | 13 | 0.50 | 0.25 (-50%) | 0.25 (-50%) |
| 14 | 0.25 | 0.25 (0%) | 0.13 (-50%) | |
| 15 | 1.00 | 1.00 (0%) | 4.00 (300%) | |
| N. glabratus | 16 | 1.00 | 16.00 (1500%) | 6.00 (500%) |
| 17 | 16.00 | 16.00 (0%) | 16.00 (0%) | |
| 18 ** | 1.00 | 0.38 (-63%) | 0.25 (-75%) |