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Fusobacterium nucleatum Promotes Bladder Cancer Development Through lncRNA IDH1-AS1-Mediated Autophagy Cover

Fusobacterium nucleatum Promotes Bladder Cancer Development Through lncRNA IDH1-AS1-Mediated Autophagy

Archivum Immunologiae et Therapiae Experimentalis
Volume 74 (2026): Issue 1 (January 2026)
By: ,  ,  ,  ,  ,   and    
Open Access
|Mar 2026
Figures & tables

Figures & Tables

Fig 1.

The effects of FN infection on cell viability, proliferation, cell cycle distribution, and apoptosis. (A) Cell viability detection (CCK8 method); (B) Cell proliferation detection (EDU method); (C) Cell cycle distribution (flow cytometry); (D) Cell apoptosis detection (flow cytometry). ## Compared to the CG, P < 0.01. CG, control group; FN, Fusobacterium nucleatum.

Fig 2.

The expression level and functional role of lncRNA IDH1-AS1 in bladder cancer; (A) Expression level of IDH1-AS1 in tissues; (B) Impact of FN infection on IDH1-AS1 expression; (C) Cell viability assay; (D) EDU proliferation assay (quantitative analysis) and (E) EDU proliferation assay (fluorescence microscopy images); (F) Cell cycle distribution; and (G) Cell apoptosis detection. ## Compared to the CG, P < 0.01. **Compared to the IDH1-AS1 IG group, P < 0.01. CG, control group; FN, Fusobacterium nucleatum; ICG, inhibitor control group; lncRNAs, long noncoding RNAs; OCG, overexpression control group.

Fig 3.

IDH1-AS1 regulates autophagy in bladder cancer cells. (A) LC3-GFP-RFP autophagy flow analysis; (B) Autophagy-related protein Beclin1, p62 and LC3 expression analysis. ## Compared to the CG, P < 0.01. **Compared to the IDH1-AS1 IG group, P < 0.01. CG, control group; FN, Fusobacterium nucleatum; ICG, inhibitor control group; lncRNAs, long noncoding RNAs; OCG, overexpression control group.

Fig 4.

FN infection promotes bladder cancer progression and autophagy in vivo. (A) Tumor growth assessment (n = 5 per group); (B) Cell proliferation analysis (EDU Assay); (C) Autophagy-related protein Beclin1, p62, and LC3 expression; (D) IDH1-AS1 gene expression analysis. ## Compared to the CG, P < 0.01. CG, control group; FN, Fusobacterium nucleatum.

Fig 5.

Effects of IDH1-AS1 expression modulation on bladder cancer progression in nude mouse xenograft models. (A) Representative images of tumor xenografts and analysis of tumor volume and weight measurements (n = 5 per group); (B) Cell proliferation visualization (EDU fluorescence microscopy); (C) Cell proliferation quantification; (D) Autophagy-related protein Beclin1, p62, and LC3 expression analysis; and (E) IDH1-AS1 gene expression analysis. ##Compared to the CG, P < 0.01. **Compared to the IDH1-AS1 IG group, P < 0.01. CG, control group; ICG, inhibitor control group; OCG, overexpression control group.
DOI: https://doi.org/10.2478/aite-2026-0011 | Journal eISSN: 1661-4917
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Language: English
Submitted on: Sep 23, 2025
Accepted on: Dec 4, 2025
Published on: Mar 31, 2026
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Fusobacterium nucleatum,
Bladder cancer,
lncRNA IDH1-AS1,
Autophagy
Related subjects:
Medicine,
Basic medical science,
Biochemistry,
Immunology,
Clinical medicine,
Clinical medicine, other,
Clinical chemistry

© 2026 JunJie Hu, BiYan Li, XiaoYuan Xu, ShuYing Lin, Shui Xu, YueJun Chen, GaoYin Tong, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 74 (2026): Issue 1 (January 2026)
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