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RFWD3 Knockdown Inhibits Cancer Cell Proliferation, Migration, and Invasion while Promoting Apoptosis of Non-Small Cell Lung Cancer Cells Cover

RFWD3 Knockdown Inhibits Cancer Cell Proliferation, Migration, and Invasion while Promoting Apoptosis of Non-Small Cell Lung Cancer Cells

Archivum Immunologiae et Therapiae Experimentalis
Volume 73 (2025): Issue 1 (January 2025)
By: Ting Ma and  Baolan Wang  
Open Access
|Jun 2025

Figures & Tables

Fig 1.

RFWD3 is highly expressed in NSCLC. (a) The differential expression of RFWD3 in normal tissues and NSCLC tissues and analysis using the GEPIA database. (b) Expression levels of RFWD3 in BEAS-2B, HCC-827, A549, NCI–H460, and H1975 cell lines. Values are presented as mean ± SD. ***p < 0.001 vs. BEAS-2B group. n = 3. NSCLC, non-small cell lung cancer; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.
RFWD3 is highly expressed in NSCLC. (a) The differential expression of RFWD3 in normal tissues and NSCLC tissues and analysis using the GEPIA database. (b) Expression levels of RFWD3 in BEAS-2B, HCC-827, A549, NCI–H460, and H1975 cell lines. Values are presented as mean ± SD. ***p < 0.001 vs. BEAS-2B group. n = 3. NSCLC, non-small cell lung cancer; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.

Fig 2.

Knockdown of RFWD3 promotes apoptosis in NSCLC cells. (a) Expression of RFWD3 in HCC-827 and NCI–H460 cells following RFWD3 knockdown. (b) Survival rate of HCC-827 and NCI–H460 cells after RFWD3 knockdown. (c) The apoptosis rate of HCC-827 and NCI–H460 cells after RFWD3 knockdown. (d, e) Protein expression levels of BAX, BCL-2, and cleaved-caspase-3 in HCC-827 and NCI–H460 cells following RFWD3 knockdown. Values are presented as mean ± SD. *p < 0.05, ***p < 0.001 vs. si-NC group. n = 3. NC, negative control; NSCLC, non-small cell lung cancer; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.
Knockdown of RFWD3 promotes apoptosis in NSCLC cells. (a) Expression of RFWD3 in HCC-827 and NCI–H460 cells following RFWD3 knockdown. (b) Survival rate of HCC-827 and NCI–H460 cells after RFWD3 knockdown. (c) The apoptosis rate of HCC-827 and NCI–H460 cells after RFWD3 knockdown. (d, e) Protein expression levels of BAX, BCL-2, and cleaved-caspase-3 in HCC-827 and NCI–H460 cells following RFWD3 knockdown. Values are presented as mean ± SD. *p < 0.05, ***p < 0.001 vs. si-NC group. n = 3. NC, negative control; NSCLC, non-small cell lung cancer; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.

Fig 3.

Knockdown of RFWD3 inhibits the migration and invasion of NSCLC cells. (a) Migration and invasion cell numbers of HCC-827 and NCI–H460 cells after RFWD3 knockdown. (b) Protein expression levels of MMP-2 and MMP-9 in HCC-827 and NCI-H460 cells after RFWD3 knockdown. Values are presented as mean ± SD. ***p < 0.001 vs. si-NC group. n = 3. NC, negative control; NSCLC, non-small cell lung cancer; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.
Knockdown of RFWD3 inhibits the migration and invasion of NSCLC cells. (a) Migration and invasion cell numbers of HCC-827 and NCI–H460 cells after RFWD3 knockdown. (b) Protein expression levels of MMP-2 and MMP-9 in HCC-827 and NCI-H460 cells after RFWD3 knockdown. Values are presented as mean ± SD. ***p < 0.001 vs. si-NC group. n = 3. NC, negative control; NSCLC, non-small cell lung cancer; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.

Fig 4.

Knockdown of RFWD3 inhibits the ERK/p38 signaling pathway. The protein expression levels of Erk, p-Erk, p38, and p-p38 in HCC-827 and NCI–H460 cells after RFWD3 knockdown. Values are presented as mean ± SD. **p < 0.01, ***p < 0.001 vs. si-NC group. n = 3. NC, negative control; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.
Knockdown of RFWD3 inhibits the ERK/p38 signaling pathway. The protein expression levels of Erk, p-Erk, p38, and p-p38 in HCC-827 and NCI–H460 cells after RFWD3 knockdown. Values are presented as mean ± SD. **p < 0.01, ***p < 0.001 vs. si-NC group. n = 3. NC, negative control; RFWD3, RING finger and WD repeat domain 3; SD, standard deviation.
DOI: https://doi.org/10.2478/aite-2025-0019 | Journal eISSN: 1661-4917
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Language: English
Submitted on: Feb 20, 2025
Accepted on: Apr 15, 2025
Published on: Jun 17, 2025
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
non-small cell lung cancer,
RFWD3,
apoptosis,
migration and invasion,
ERK/p38
Related subjects:
Medicine,
Basic medical science,
Biochemistry,
Immunology,
Clinical medicine,
Clinical medicine, other,
Clinical chemistry

© 2025 Ting Ma, Baolan Wang, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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