Unraveling the Complexity and Advancements of Transdifferentiation Technologies in the Biomedical Field and Their Potential Clinical Relevance
References
- Ambasudhan R, Talantova M, Coleman R et al. (2011) Direct reprogramming of adult human fibroblasts to functional neurons under defined conditions. Cell Stem Cell 9:113–118.
https://doi.org/10.1016/j.stem.2011.07.002 - Arnan C, Ullrich S, Pulido-Quetglas C et al. (2022) Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages. BMC Genomics 23:402.
https://doi.org/10.1186/s12864-022-08612-7 - Ban H, Nishishita N, Fusaki N et al. (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci USA 108:14234–14239.
https://doi.org/10.1073/pnas.1103509108 - Bar-Nur O, Russ HA, Efrat S et al. (2011) Epigenetic memory and preferential lineage-specific differentiation in induced pluripotent stem cells derived from human pancreatic islet beta cells. Cell Stem Cell 9:17–23.
https://doi.org/10.1016/j.stem.2011.06.007 - Becker JS, Nicetto D, Zaret KS (2016) H3K9me3-dependent heterochromatin: Barrier to cell fate changes. Trends Genet 32:29–41.
https://doi.org/10.1016/j.tig.2015.11.001 - Ben-Zwi M, Petrou P, Halimi M et al. (2019) Neuralized mesenchymal stem cells (NMSC) exhibit phenotypical, and biological evidence of neuronal transdifferentiation and suppress EAE more effectively than unmodified MSC. Immunol Lett 212:6–13.
https://doi.org/10.1016/j.imlet.2019.05.009 - Cahan P, Li H, Morris SA, et al. (2014) CellNet: network biology applied to stem cell engineering. 158:903–915.
https://doi.org/10.1016/j.cell.2014.07.020 - Chaudhary PK, Saini D, Mishra P et al. (2024) Essential oil active constituents loaded PVA nanofibers enhance antibiofilm activity against Candida albicans and Candida tropicalis. J Drug Deliv Sci Technol 98:105871.
https://doi.org/10.1016/j.jddst.2024.105871 - Chen J, Sun N, Li F et al. (2023) Carnosol alleviates collagen-induced arthritis by inhibiting Th17-mediated immunity and favoring suppressive activity of regulatory T cells. Biomed Res Int 2023:1179973.
https://doi.org/10.1155/2023/1179973 - Chen J, Wang C, Kuang S (2019) Transdifferentiation of muscle satellite cells to adipose cells using CRISPR/Cas9-mediated targeting of MyoD. Methods Mol Biol 1889:25–41.
https://doi.org/10.1007/978-1-4939-8897-6_3 - Chen PY, Qin L, Li G et al. (2020) Smooth muscle cell reprogramming in aortic aneurysms. Cell Stem Cell 26:542.e–557.e.
https://doi.org/10.1016/j.stem.2020.02.013 - Cheng L, Hu W, Qiu B et al. (2014) Generation of neural progenitor cells by chemical cocktails and hypoxia. Cell Res 24:665–679.
https://doi.org/10.1038/cr.2014.32 - Choudhury S, Madhu Krishna M, Sen D et al. (2024) 3D porous polymer Scaffold-conjugated KGF-mimetic peptide promotes functional skin regeneration in chronic diabetic wounds. ACS Appl Mater Interfaces 16:37418–37434.
https://doi.org/10.1021/acsami.4c02633 - Christoffers S, Seiler L, Wiebe E et al. (2024) Possibilities and efficiency of MSC co-transfection for gene therapy. Stem Cell Res Ther 15:150.
https://doi.org/10.1186/s13287-024-03757-6 - Chu WT, Chu X, Wang J (2022) Uncovering the quantitative relationships among chromosome fluctuations, epigenetics, and gene expressions of transdifferentiation on Waddington landscape. Adv Sci 9:e2103617.
https://doi.org/10.1002/advs.202103617 - Cieślar-Pobuda A, Knoflach V, Ringh MV et al. (2017) Transdifferentiation and reprogramming: Overview of the processes, their similarities and differences. Biochim Biophys Acta Mol Cell Res 1864:1359–1369.
https://doi.org/10.1016/j.bbamcr.2017.04.017 - Cieślar-Pobuda A, Rafat M, Knoflach V et al. (2016) Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells. Oncotarget 7:42314–42329.
https://doi.org/10.18632/oncotarget.9791 - Dabral S, Noh M, Werner F et al. (2024) C-type natriuretic peptide/cGMP/FoxO3 signaling attenuates hyperproliferation of pericytes from patients with pulmonary arterial hypertension. Commun Biol 7:693.
https://doi.org/10.1038/s42003-024-06375-3 - Dai W, Xu X, Li S et al. (2017) SOX4 promotes proliferative signals by regulating glycolysis through AKT activation in melanoma cells. J Invest Dermatol 137:2407–2416.
https://doi.org/10.1016/j.jid.2017.06.026 - Darcy A, Meltzer M, Miller J et al. (2012) A novel library screen identifies immunosuppressors that promote osteoblast differentiation. Bone 50:1294–1303.
https://doi.org/10.1016/j.bone.2012.03.001 - Davis R, Weintraub H, Lassar A (1987) Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell 51: 987–1000.
https://doi.org/10.1016/0092-8674(87)90585-x - Deng J, Luo K, Xu P et al. (2021) High-efficiency c-Myc-mediated induction of functional hepatoblasts from the human umbilical cord mesenchymal stem cells. Stem Cell Res Ther 12:375.
https://doi.org/10.1186/s13287-021-02419-1 - Deng Q, Ramsköld D, Reinius B et al. (2014) Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells. Science 343:193–196.
https://doi.org/10.1126/science.1245316 - Di Y, Zhang M, Chen Y et al. (2022) Catalpol inhibits Tregs-to-Th17 cell transdifferentiation by up-regulating Let-7g-5p to reduce STAT3 protein levels. Yonsei Med J 63:56–65.
https://doi.org/10.3349/ymj.2022.63.1.56 - Du J, Wang Z, Liu X et al. (2023) Improving Schwann cell differentiation from human adipose stem cells with metabolic glycoengineering. Cells 12:1190.
https://doi.org/10.3390/cells12081190 - Farrim MI, Gomes A, Milenkovic D et al. (2024) Gene expression analysis reveals diabetes-related gene signatures. Hum Genomics 18:16.
https://doi.org/10.1186/s40246-024-00582-z - Faustino D, Brinkmeier H, Logotheti S et al. (2022) Novel integrated workflow allows production and in-depth quality assessment of multifactorial reprogrammed skeletal muscle cells from human stem cells. Cell Mol Life Sci 79:229.
https://doi.org/10.1007/s00018-022-04264-8 - Fu Y, Huang C, Xu X et al. (2015) Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails. Cell Res 25:1013–1024.
https://doi.org/10.1038/cr.2015.99 - Gong J, Tang D, Leong K (2018) CRISPR/dCas9-mediated cell differentiation. Curr Opin Biomed Eng 7:9–15.
https://doi.org/10.1016/j.cobme.2018.08.005 - Grath A, Dai G (2019) Direct cell reprogramming for tissue engineering and regenerative medicine. J Biol Eng 13:14.
https://doi.org/10.1186/s13036-019-0144-9 - Grath A, Dai G (2024) SOX17/ETV2 improves the direct reprogramming of adult fibroblasts to endothelial cells. Cell Rep Methods 4:100732.
https://doi.org/10.1016/j.crmeth.2024.100732 - Gu X, Wang J, Jiang X (2022) miR-124- and let-7-mediated reprogram of human fibroblasts into SST interneurons. ACS Chem Neurosci 13:2755–2765.
https://doi.org/10.1021/acschemneuro.2c00445 - Guerrero-Ramirez GI, Valdez-Cordoba CM, Islas-Cisneros JF et al. (2018) Computational approaches for predicting key transcription factors in targeted cell reprogramming. Mol Med Rep 18:1225–1237.
https://doi.org/10.3892/mmr.2018.9092 - Guo T, Wang J, Pang M et al. (2024a) Reprogramming and multi-lineage transdifferentiation attenuate the tumorigenicity of colorectal cancer cells. J Biol Chem 300:105534.
https://doi.org/10.1016/j.jbc.2023.105534 - Guo X, Wang C, Zhang Y et al. (2024b) Cell-fate conversion of intestinal cells in adult Drosophila midgut by depleting a single transcription factor. Nat Commun 15:2656.
https://doi.org/10.1038/s41467-024-46956-8 - Guo Z, Zhang L, Wu Z et al. (2014) In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer’s disease model. Cell Stem Cell 14:188–202.
https://doi.org/10.1016/j.stem.2013.12.001 - Gurunathan S, Kim JH (2017) Graphene oxide–silver nanoparticles nanocomposite stimulates differentiation in human neuroblastoma cancer cells (SH-SY5Y). Int J Mol Sci 18:2549.
https://doi.org/10.3390/ijms18122549 - Hammelman J, Gifford DK (2021) Discovering differential genome sequence activity with interpretable and efficient deep learning. PLoS Comput Biol 17:e1009282.
https://doi.org/10.1371/journal.pcbi.1009282 - Hammelman J, Patel T, Closser M et al. (2022) Ranking reprogramming factors for cell differentiation. Nat Methods 19:812–822.
https://doi.org/10.1038/s41592-022-01522-2 - Hasani S, Javeri A, Asadi A et al. (2020) Cardiac differentiation of adipose tissue-derived stem cells is driven by BMP4 and bFGF but counteracted by 5-azacytidine and valproic acid. Cell J 22:273–282.
https://doi.org/10.22074/cellj.2020.6582 - He Y, Ji Z, Gong Y et al. (2023) Numb/Parkin-directed mitochondrial fitness governs cancer cell fate via metabolic regulation of histone lactylation. Cell Rep 42:112033.
https://doi.org/10.1016/j.celrep.2023.112033 - He ZQ, Li YH, Feng GH et al. (2022) Pharmacological perturbation of mechanical contractility enables robust transdifferentiation of human fibroblasts into neurons. Adv Sci (Weinh) 9:e2104682.
https://doi.org/10.1002/advs.202104682 - Herdy J, Schafer S, Kim Y et al. (2019) Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife 8:e41356.
https://doi.org/10.7554/eLife.41356 - Hirai T, Yasuda S, Umezawa A et al. (2023) Country-specific regulation and international standardization of cell-based therapeutic products derived from pluripotent stem cells. Stem Cell Reports 18:1573–1591.
https://doi.org/10.1016/j.stemcr.2023.05.003 - Hong X, Margariti A, Le Bras A et al. (2017) Transdifferentiated human vascular smooth muscle cells are a new potential cell source for endothelial regeneration. Sci Rep 7:5590.
https://doi.org/10.1038/s41598-017-05665-7 - Hu B, Zhang X, Fan H et al. (2024) FOXF1 reverses lung fibroblasts transdifferentiation via inhibiting TGF-β/SMAD2/3 pathway in silica-induced pulmonary fibrosis. Int Immunopharmacol 133:112067.
https://doi.org/10.1016/j.intimp.2024.112067 - Hu W, Qiu B, Guan W et al. (2015a) Direct conversion of normal and Alzheimer’s disease human fibroblasts into neuronal cells by small molecules. Cell Stem Cell 17:204–212.
https://doi.org/10.1016/j.stem.2015.07.006 - Hu W, Qiu B, Guan W et al. (2015b) Direct conversion of normal and Alzheimer’s disease human fibroblasts into neuronal cells by small molecules. Cell Stem Cell 17:204–212.
https://doi.org/10.1016/j.stem.2015.07.006 - Hu X, Wang X, Xu Y et al. (2020) Electric conductivity on aligned nanofibers facilitates the transdifferentiation of mesenchymal stem cells into Schwann cells and regeneration of injured peripheral nerve. Adv Healthc Mater 9:e1901570.
https://doi.org/10.1002/adhm.201901570 - Huang H, Zhang W, Zhang J et al. (2023) Epigenome editing based on CRISPR/dCas9p300 facilitates transdifferentiation of human fibroblasts into Leydig-like cells. Exp Cell Res 425:113551.
https://doi.org/10.1016/j.yexcr.2023.113551 - Inagawa K, Miyamoto K, Yamakawa H et al. (2012) Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ Res 111:1147–1156.
https://doi.org/10.1161/CIRCRESAHA.112.271148 - Islam S, Zeisel A, Joost S et al. (2014) Quantitative single-cell RNA-seq with unique molecular identifiers. Nat Methods 11:-163–166.
https://doi.org/10.1038/nmeth.2772 - Jalili A, Shojaei-Ghahrizjani F, Tabatabaiefar MA et al. (2024) Decellularized skin pretreatment by monophosphoryl lipid A and Lactobacillus casei supernatant accelerate skin recellularization. Mol Biol Rep 51:675.
https://doi.org/10.1007/s11033-024-09599-y - Jayawardena TM, Egemnazarov B, Finch EA et al. (2012) MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes. Circ Res 110:1465–1473.
https://doi.org/10.1161/CIRCRESAHA.112.269035 - Jayawardena TM, Finch EA, Zhang L et al. (2015) MicroRNA induced cardiac reprogramming in vivo: Evidence for mature cardiac myocytes and improved cardiac function. Circ Res 116:418–424.
https://doi.org/10.1161/CIRCRESAHA.116.304510 - Jiang MQ, Yu SP, Estaba T et al. (2024) Reprogramming glioblastoma cells into non-cancerous neuronal cells as a novel anti-cancer strategy. Cells 13:897.
https://doi.org/10.3390/cells13110897 - Jiang W, Yang Y, Mercer-Smith AR et al. (2021) Development of next-generation tumor-homing induced neural stem cells to enhance treatment of metastatic cancers. Sci Adv 7:eabf1526.
https://doi.org/10.1126/sciadv.abf1526 - Jing N, Tao Z, Du X et al. (2024) Targeting SOX4/PCK2 signaling suppresses neuroendocrine transdifferentiation of castration-resistant prostate cancer. Biol Direct 19:56.
https://doi.org/10.1186/s13062-024-00500-2 - Kaur K, Yang J, Eisenberg CA et al. (2014) 5-azacytidine promotes the transdifferentiation of cardiac cells to skeletal myocytes. Cell Reprogram 16:324–330.
https://doi.org/10.1089/cell.2014.0021 - Kim K, Doi A, Wen B et al. (2010) Epigenetic memory in induced pluripotent stem cells. Nature 467:285–290.
https://doi.org/10.1038/nature09342 - Kim SHL, Lee SS, Kim I et al. (2020) Ectopic transient overexpression of OCT-4 facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells. J Tissue Eng 11:2041731420909208.
https://doi.org/10.1177/2041731420909208 - Kim WJ, Bae J, Lee EH et al. (2024) Long noncoding RNA MALAT1 mediates fibrous topography-driven pathologic calcification through transdifferentiation of myoblasts. Mater Today Bio 28:101182.
https://doi.org/10.1016/j.mtbio.2024.101182 - Kim Y, Kang K, Lee SB et al. (2019) Small molecule-mediated reprogramming of human hepatocytes into bipotent progenitor cells. J Hepatol 70:97–107.
https://doi.org/10.1016/j.jhep.2018.09.007 - Komuta Y, Ishii T, Kaneda M et al. (2016) In vitro transdifferentiation of human peripheral blood mononuclear cells to photoreceptor-like cells. Biol Open 5:709–719.
https://doi.org/10.1242/bio.016477 - Korman B (2019) Evolving insights into the cellular and molecular pathogenesis of fibrosis in systemic sclerosis. Transl Res 209:77–89.
https://doi.org/10.1016/j.trsl.2019.02.010 - Kraus EE, Kakuk-Atkins L, Farinas MF et al. (2021) Regulation of autoreactive CD4 T cells by FoxO1 signaling in CNS autoimmunity. J Neuroimmunol 359:577675.
https://doi.org/10.1016/j.jneuroim.2021.577675 - Labarrade F, Botto JM, Imbert IM (2022) miR-203 represses keratinocyte stemness by targeting survivin. J Cosmet Dermatol 21:6100–6108.
https://doi.org/10.1111/jocd.15147 - Lang H, Lin N, Chen X et al. (2024) Repressing miR-23a promotes the transdifferentiation of pancreatic α cells to β cells via negatively regulating the expression of SDF-1α. PLoS One 19:e0299821.
https://doi.org/10.1371/journal.pone.0299821 - Lardon J, Huyens N, Rooman I et al. (2004) Exocrine cell transdifferentiation in dexamethasone-treated rat pancreas. Virchows Arch 444:61–65.
https://doi.org/10.1007/s00428-003-0930-z - Lee B, Roh JS, Jeong H et al. (2024) Ginkgo biloba extract ameliorates skin fibrosis in a bleomycin-induced mouse model of systemic sclerosis. Anim Cells Syst (Seoul) 28:152–160.
https://doi.org/10.1080/19768354.2024.2337761 - Li B, Jiang H, Li H et al. (2021) Direct conversion of adult human retinal pigmented epithelium cells to neurons with photoreceptor properties. Exp Biol Med (Maywood) 246:240–248.
https://doi.org/10.1177/1535370220963755 - Li H, Xing H (2023) Interleukin-35 enhances regulatory T cell function by potentially suppressing their transdifferentiation into a T helper 17-like phenotype in Kawasaki disease. Immunol Invest 52:513–528.
https://doi.org/10.1080/08820139.2023.2201283 - Li X, Zuo X, Jing J et al. (2015) Small-molecule-driven direct reprogramming of mouse fibroblasts into functional neurons. Cell Stem Cell 17:195–203.
https://doi.org/10.1016/j.stem.2015.06.003 - Li Y, Jie W, Qi Y et al. (2024) Inhibition of RIPK1 alleviating vascular smooth muscle cells osteogenic transdifferentiation via Runx2. iScience 27:108766.
https://doi.org/10.1016/j.isci.2023.108766 - Lin TH, Wang HC, Tseng YL et al. (2024a) A bioactive composite scaffold enhances osteochondral repair by using thermosensitive chitosan hydrogel and endothelial lineage cell-derived chondrogenic cell. Mater Today Bio 28:101174.
https://doi.org/10.1016/j.mtbio.2024.101174 - Lin X, Dai Z, Tasiheng Y et al. (2024b) BCL6 overexpression in CD4+ T cells induces Tfh-like transdifferentiation and enhances antitumor efficiency of CAR-T therapy in pancreatic cancer. Biochim Biophys Acta Mol Basis Dis 1870:167346.
https://doi.org/10.1016/j.bbadis.2024.167346 - Liu C, Medina P, Thomas D et al. (2021) A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity. STAR Protoc 2:100556.
https://doi.org/10.1016/j.xpro.2021.100556 - Liu Z, Wang L, Welch JD et al. (2017) Single-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte. Nature 551:100–104.
https://doi.org/10.1038/nature24454 - Lu J, Meng J, Wu G et al. (2024) Th1 cells reduce the osteoblast-like phenotype in valvular interstitial cells by inhibiting NLRP3 IFNlammasome activation in macrophages. Mol Med 30:110.
https://doi.org/10.1186/s10020-024-00882-z - Lu NZ, Wardell SE, Burnstein KL et al. (2006) International Union of Pharmacology. LXV. The pharmacology and classification of the nuclear receptor superfamily: Glucocorticoid, mineralocorticoid, progesterone, and androgen receptors. Pharmacol Rev 58: 782–797.
https://doi.org/10.1124/pr.58.4.9 - Ma R, Lu D, Xie Q et al. (2023) l-Borneol and d-Borneol promote transdifferentiation of astrocytes into neurons in rats by regulating Wnt/Notch pathway to exert neuroprotective effect during recovery from cerebral ischaemia. Phymomedicine 109:154583.
https://doi.org/10.1016/j.phymed.2022.154583 - Ma T, Ren R, Lv J et al. (2024) Transdifferentiation of fibroblasts into muscle cells to constitute cultured meat with tunable intramuscular fat deposition. Elife 13:R93220.
https://doi.org/10.7554/eLife.93220 - Mahdi A, Mehrdad A, Safoura K, et al (2023) Positive effect of miR-2392 on fibroblast to cardiomyocyte-like cell fate transition: An in silico and in vitro study. Gene 879:147598.
https://doi.org/10.1016/j.gene.2023.147598 - Martin CA, Radhakrishnan S, Gómez Ribelles JL et al. (2022) Adipose tissue derived stromal cells in a gelatin-based 3D matrix with exclusive ascorbic acid signalling emerged as a novel neural tissue engineering construct: An innovative prototype for soft tissue. Regen Biomater 9:rbac031.
https://doi.org/10.1093/rb/rbac031 - Mendieta I, Rodríguez-Nieto M, Nuñez-Anita RE et al. (2021) Ultrastructural changes associated to the neuroendocrine transdifferentiation of the lung adenocarcinoma cell line A549. Acta Histochem 123:151797.
https://doi.org/10.1016/j.acthis.2021.151797 - Meng S, Chanda P, Thandavarayan RA et al. (2017) Transflammation: Innate immune signaling in nuclear reprogramming. Adv Drug Deliv Rev 120:133–141.
https://doi.org/10.1016/j.addr.2017.09.010 - Mishra P, Gupta P, Srivastava AK et al. (2021) Eucalyptol/β-cyclodextrin inclusion complex loaded gellan/PVA nanofibers as antifungal drug delivery system. Int J Pharm 609:121163.
https://doi.org/10.1016/j.ijpharm.2021.121163 - Mishra P, Gupta P, Srivastava R et al. (2023) Exploration of antibiofilm and in vivo wound healing activity of p-cymene-loaded gellan/PVA nanofibers. ACS Appl Bio Mater 6:1816–1831.
https://doi.org/10.1021/acsabm.3c00047 - Mishra P, Srivastava AK, Yadav TC et al. (2022) Advances in natural polymer-based electrospun nanomaterials for soft tissue engineering. In: Sarma H, Gupta S, Narayan M et al. (ed) Engineered nanomaterials for innovative therapies and biomedicine. Springer, Cham. pp 29–52.
https://doi.org/10.1007/978-3-030-82918-6_2 - Morita R, Suzuki M, Kasahara H et al. (2015) ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells. Proc Natl Acad Sci USA 112:160–165.
https://doi.org/10.1073/pnas.1413234112 - Muniyandi P, Palaninathan V, Mizuki T et al. (2021) Scaffold mediated delivery of dual miRNAs to transdifferentiate cardiac fibroblasts. Mater Sci Eng C Mater Biol Appl 128:112323.
https://doi.org/10.1016/j.msec.2021.112323 - Ng TK, Yung JS, Choy KW et al. (2015) Transdifferentiation of periodontal ligament-derived stem cells into retinal ganglion-like cells and its microRNA signature. Sci Rep 5:16429.
https://doi.org/10.1038/srep16429 - Nilsson G, Kannius-Janson M (2016) Forkhead Box F1 promotes breast cancer cell migration by upregulating lysyl oxidase and suppressing SMAD2/3 signaling. BMC Cancer 16:142.
https://doi.org/10.1186/s12885-016-2196-2 - Ninomiya I, Koyama A, Otsu Y et al. (2023) Regeneration of the cerebral cortex by direct chemical reprogramming of macrophages into neuronal cells in acute ischemic stroke. Front Cell Neurosci 17:1225504.
https://doi.org/10.3389/fncel.2023.1225504 - Ouyang JF, Kamaraj US, Polo JM et al. (2019) Molecular interaction networks to select factors for cell conversion. Methods Mol Biol 1975:333–361.
https://doi.org/10.1007/978-1-4939-9224-9_16 - Pan H, Xue C, Auerbach BJ et al. (2020) Single-cell genomics reveals a novel cell state during smooth muscle cell phenotypic switching and potential therapeutic targets for atherosclerosis in mouse and human. Circulation 142:2060–2075.
https://doi.org/10.1161/CIRCULATIONAHA.120.048378 - Patel M, Yang S (2010) Advances in reprogramming somatic cells to induced pluripotent stem cells. Stem Cell Rev Rep 6:367–380.
https://doi.org/10.1007/s12015-010-9123-8 - Patel R, Parmar N, Rathwa N et al. (2022a) A novel therapeutic combination of sitagliptin and melatonin regenerates pancreatic β-cells in mouse and human islets. Biochim Biophys Acta Mol Cell Res 1869:119263.
https://doi.org/10.1016/j.bbamcr.2022.119263 - Patel T, Hammelman J, Aziz S et al. (2022b) Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro. Nat Commun 13:5427.
https://doi.org/10.1038/s41467-022-33022-4 - Qian L, Huang Y, Spencer CI et al. (2012) In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature 485:593–598.
https://doi.org/10.1038/nature11044 - Qin Z, Huang Y, Li Z et al. (2023) Glioblastoma vascular plasticity limits effector T-cell IFNiltration and is blocked by cAMP activation. Cancer Immunol Res 11:1351–1366.
https://doi.org/10.1158/2326-6066.CIR-22-0872 - Quintanal-Villalonga A, Kawasaki K, Redin E et al. (2024) CDC7 inhibition impairs neuroendocrine transformation in lung and prostate tumors through MYC degradation. Signal Transduct Target Ther 9:189.
https://doi.org/10.1038/s41392-024-01908-y - Raval Z, Losordo DW (2013) Cell therapy of peripheral arterial disease: From experimental findings to clinical trials. Circ Res 112:1288–1302.
https://doi.org/10.1161/CIRCRESAHA.113.300565 - Reid A, Tursun B (2018) Transdifferentiation: Do transition states lie on the path of development? Curr Opin Syst Biol 11:18–23.
https://doi.org/10.1016/j.coisb.2018.07.004 - Richards DJ, Li Y, Kerr CM et al. (2020) Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity. Nat Biomed Eng 4:446–462.
https://doi.org/10.1038/s41551-020-0539-4 - Saaoud F, Liu L, Xu K et al. (2023) Aorta-and liver-generated TMAO enhances trained immunity for increased IFNlammation via ER stress/mitochondrial ROS/glycolysis pathways. JCI Insight 8:e158183.
https://doi.org/10.1172/jci.insight.158183 - Saliba AE, Westermann AJ, Gorski SA et al. (2014) Single-cell RNA-seq: Advances and future challenges. Nucleic Acids Res 42:8845–8860.
https://doi.org/10.1093/nar/gku555 - Sarnobat D, Moffett CR, Tanday N et al. (2020) Antidiabetic drug therapy alleviates type 1 diabetes in mice by promoting pancreatic α-cell transdifferentiation. Biochem Pharmacol 182:114216.
https://doi.org/10.1016/j.bcp.2020.114216 - Sato Y, Bando H, Di Piazza M et al. (2019) Tumorigenicity assessment of cell therapy products: The need for global consensus and points to consider. Cytotherapy 21:1095–1111.
https://doi.org/10.1016/j.jcyt.2019.10.001 - Sayed N, Wong WT, Ospino F et al. (2015) Transdifferentiation of human fibroblasts to endothelial cells: Role of innate immunity. Circulation 131:300–309.
https://doi.org/10.1161/CIRCULATIONAHA.113.007394 - Sharifi-Kelishadi M, Zare L, Fathollahi Y et al. (2024) Conversion of astrocyte cell lines to oligodendrocyte progenitor cells using small molecules and transplantation to animal model of multiple sclerosis. J Mol Neurosci 74:40.
https://doi.org/10.1007/s12031-024-02206-6 - Shen E, Piao M, Li Y et al. (2024) CMTM3 suppresses proliferation and osteogenic transdifferentiation of C2C12 myoblasts through p53 upregulation. Cells 13:1352.
https://doi.org/10.3390/cells13161352 - Shen K, Wu D, Sun B et al. (2023) Ginsenoside Rg1 promotes astrocyte-to-neuron transdifferentiation in rat and its possible mechanism. CNS Neurosci Ther 29:256–269.
https://doi.org/10.1111/cns.14000 - Shi C, Zhang J, Wang H et al. (2023) Trojan horse nanocapsule enabled in situ modulation of the phenotypic conversion of Th17 cells to Treg cells for the treatment of multiple sclerosis in mice. Adv Mater 35:e2210262.
https://doi.org/10.1002/adma.202210262 - Shiode Y, Kodama T, Shigeno S et al. (2023) TNF receptor–related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF-κβ-inducing kinase–mediated hepatocyte transdifferentiation. Hepatology 77:395–410.
https://doi.org/10.1002/hep.32317 - Singh VP, Pinnamaneni JP, Pugazenthi A et al. (2020) Enhanced generation of induced cardiomyocytes using a small-molecule cocktail to overcome barriers to cardiac cellular reprogramming. J Amr Heart Assoc 9:e015686.
https://doi.org/10.1161/JAHA.119.015686 - Skubis A, Gola J, Sikora B et al. (2017) Impact of antibiotics on the proliferation and differentiation of human adipose-derived mesenchymal stem cells. Int J Mol Sci 18:2522.
https://doi.org/10.3390/ijms18122522 - Smith ZD, Sindhu C, Meissner A (2016) Molecular features of cellular reprogramming and development. Nat Rev Mol Cell Biol 17: 139–154.
https://doi.org/10.1038/nrm.2016.6 - Song Z, Cao Q, Guo B et al. (2023) Overexpression of RACGAP1 by E2F1 promotes neuroendocrine differentiation of prostate cancer by stabilizing EZH2 expression. Aging Dis 14:1757–1774.
https://doi.org/10.14336/AD.2023.0202 - Sorraksa N, Kaokaen P, Kunhorm P et al. (2024) Rapid induction of dopaminergic neuron-like cells from human fibroblasts by autophagy activation with only 2-small molecules. 3 Biotec 14:115.
https://doi.org/10.1007/s13205-024-03957-0 - Spezani R, Reis-Barbosa PH, Mandarim-de-Lacerda CA (2024)Update on the transdifferentiation of pancreatic cells into functional beta cells for treating diabetes. Life Sci 346:122645.
https://doi.org/10.1016/j.lfs.2024.122645 - Szabo E, Rampalli S, Risueño RM et al. (2010) Direct conversion of human fibroblasts to multilineage blood progenitors. Nature 468:521–526.
https://doi.org/10.1038/nature09591 - Takahashi K, Narita M, Yokura M et al. (2009) Human induced pluripotent stem cells on autologous feeders. PLoS One 4:e8067.
https://doi.org/10.1371/journal.pone.0008067 - Takahashi K, Tanabe K, Ohnuki M et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872.
https://doi.org/10.1016/j.cell.2007.11.019 - Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676.
https://doi.org/10.1016/j.cell.2006.07.024 - Torper O, Pfisterer U, Wolf DA et al. (2013) Generation of induced neurons via direct conversion in vivo. Proc Natl Acad Sci USA 110:7038–7043.
https://doi.org/10.1073/pnas.1303829110 - Tran ON, Wang H, Li S et al. (2022) Organ-specific extracellular matrix directs transdifferentiation of mesenchymal stem cells and formation of salivary gland-like organoids in vivo. Stem Cell Res Ther 13:306.
https://doi.org/10.1186/s13287-022-02993-y - Ullah M, Sittinger M, Ringe J (2014) Transdifferentiation of adipogenically differentiated cells into osteogenically or chondrogenically differentiated cells: Phenotype switching via dedifferentiation. Int J Biochem Cell Biol 46:124–137.
https://doi.org/10.1016/j.biocel.2013.11.010 - Venugopal B, Shenoy SJ, Mohan S et al. (2020) Bioengineered corneal epithelial cell sheet from mesenchymal stem cells – A functional alternative to limbal stem cells for ocular surface reconstruction. J Biomed Mater Res B Appl Biomater 108:1033–1045.
https://doi.org/10.1002/jbm.b.34455 - Wang F, Zhang S, Sun F et al. (2024) Anti-angiogenesis and anti-immunosuppression gene therapy through targeting COUP-TFII in an in situ glioblastoma mouse model. Cancer Gene Ther 31:1135–1150.
https://doi.org/10.1038/s41417-024-00799-z - Wang H, Li X, Gao S et al. (2015) Transdifferentiation via transcription factors or microRNAs: Current status and perspective. Differentiation 90:69–76.
https://doi.org/10.1016/j.diff.2015.10.002 - Wang Y, Liu H, Zhang Z et al. (2023) G-MDSC-derived exosomes mediate the differentiation of M-MDSC into M2 macrophages promoting colitis-to-cancer transition. J Immunother Cancer 11:e006166.
https://doi.org/10.1136/jitc-2022-006166 - Wild SL, Tosh D (2021) Molecular mechanisms of transcription factor mediated cell reprogramming: Conversion of liver to pancreas. Biochem Soc Trans 49:579–590.
https://doi.org/10.1042/BST20200219 - Wolff G, Wilhelm RS (1895) Die regeneration der urodelenlinse. Arch Entw Mech Org 1:380–390.
https://doi.org/10.1007/BF02156634 - Wong E, Sangadala S, Boden SD et al. (2013) A novel low-molecular-weight compound enhances ectopic bone formation and fracture repair. J Bone Joint Surg Am 95:454–461.
https://doi.org/10.2106/JBJS.L.00275 - Woodell AS, Landoni E, Valdivia A et al. (2023) Utilizing induced neural stem cell-based delivery of a cytokine cocktail to enhance chimeric antigen receptor-modified T-cell therapy for brain cancer. Bioeng Transl Med 8:e10538.
https://doi.org/10.1002/btm2.10538 - Xi Y, Song B, Ngan I et al. (2022) Glucagon-receptor-antagonism-mediated β-cell regeneration as an effective anti-diabetic therapy. Cell Rep 39:110872.
https://doi.org/10.1016/j.celrep.2022.110872 - Xie H, Ye M, Feng R et al. (2004) Stepwise reprogramming of B cells into macrophages. Cell 117:663–676.
https://doi.org/10.1016/s0092-8674(04)00419-2 - Xie X, Fu Y, Liu J (2017) Chemical reprogramming and transdifferentiation. Curr Opin Genet Dev 46:104–113.
https://doi.org/10.1016/j.gde.2017.07.003 - Yi B, Ding T, Jiang S et al. (2021) Conversion of stem cells from apical papilla into endothelial cells by small molecules and growth factors. Stem Cell Res Ther 12:266.
https://doi.org/10.1186/s13287-021-02350-5 - Yoo AS, Staahl BT, Chen L et al. (2009) MicroRNA-mediated switching of chromatin-remodelling complexes in neural development. Nature 460:642–646.
https://doi.org/10.1038/nature08139 - Yu J, Vodyanik MA, Smuga-Otto K et al. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920.
https://doi.org/10.1126/science.1151526 - Zhang C, Li C (2021) Revealing the mechanism of lymphoid and myeloid cell differentiation and transdifferentiation through landscape quantification. Phys Rev Res 3:013186.
https://doi.org/10.1103/PhysRevResearch.3.013186 - Zhang Q, Liu W, Wang H et al. (2022) TH17 cells promote CNS IFNlammation by sensing danger signals via Mincle. Nat Commun 13:2406.
https://doi.org/10.1038/s41467-022-30174-1 - Zhang W, Wu L, Qu R et al. (2024a) Hesperidin activates the GLP-1R/cAMP-CREB/IRS2/PDX1 pathway to promote transdifferentiation of islet α cells into β cells across the spectrum. Heliyon 10:e35424.
https://doi.org/10.1016/j.heliyon.2024.e35424 - Zhang X, Taylor H, Valdivia A et al. (2024b) Auto-loaded TRAIL-exosomes derived from induced neural stem cells for brain cancer therapy. J Control Release 372:433–445.
https://doi.org/10.1016/j.jconrel.2024.06.048 - Zhang XW, Li JY, Li L et al. (2023a) Neurokinin-1 receptor drives PKCɑ-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer. Cell Death Dis 14:384.
https://doi.org/10.1038/s41419-023-05894-x - Zhang Y, Li X, Xing J et al. (2023b) Chemical transdifferentiation of somatic cells: Unleashing the power of small molecules. Biomedicines 11:2913.
https://doi.org/10.3390/biomedicines11112913 - Zhou C, Gu H, Fan R et al. (2015) MicroRNA 302/367 cluster effectively facilitates direct reprogramming from human fibroblasts into functional neurons. Stem Cells Dev 24:2746–2755.
https://doi.org/10.1089/scd.2015.0123 - Zhou P, Lu Y, Sun XH (2011) Zebularine suppresses TGF-beta-induced lens epithelial cell–myofibroblast transdifferentiation by inhibiting MeCP2. Mol Vis 17:2717–2723.
https://pmc.ncbi.nlm.nih.gov/articles/PMC3209433/ - Zhou Y, Shiok TC, Richards AM et al. (2018) MicroRNA-101a suppresses fibrotic programming in isolated cardiac fibroblasts and in vivo fibrosis following transaortic constriction. J Mol Cell Cardiol 121:266–276.
https://doi.org/10.1016/j.yjmcc.2018.07.251 - Zhu B, Fisher E, Li L et al. (2023) PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes. Stem Cell Reports 18:2268–2282.
https://doi.org/10.1016/j.stemcr.2023.09.012 - Zhu S, Russ HA, Wang X et al. (2016) Human pancreatic beta-like cells converted from fibroblasts. Nat Commun 7:10080.
https://doi.org/10.1038/ncomms10080
DOI: https://doi.org/10.2478/aite-2025-0001 | Journal eISSN: 1661-4917
Language: English
Submitted on: Sep 11, 2024
Accepted on: Nov 4, 2024
Published on: Dec 5, 2024
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
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© 2024 Purusottam Mishra, Izabella Biesiada, Payal Gupta, Saeid Ghavami, Jarosław Markowski, Marek J. Łos, published by Hirszfeld Institute of Immunology and Experimental Therapy
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