Testicular toxicity of Aroclor 1254 in selenium-deficient and selenium-supplemented rats
References
- Dobrzynska MM, Radzikowska J. The effects of Aroclor 1254 alone and in combination with X-rays on the male mice germ cells quantity and quality. Toxicology 2022;477:153273. doi: 10.1016/j.tox.2022.153273
- Murugesan P, Muthusamy T, Balasubramanian K, Arunakaran J. Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells. Toxicology 2007;232:170–82. doi: 10.1016/j.tox.2007.01.008
- Dikshith TSS, Rockwood W, Abraham R, Coulston F. Effects of a polychlorinated biphenyl (Aroclor 1254) on rat testis. Exp Mol Pathol 1975;22:376–85. doi: 10.1016/0014-4800(75)90082-9
- Murugesan P, Muthusamy T, Balasubramanian K, Arunakaran J. Studies on the protective role of vitamin C and E against polychlorinated biphenyl (Aroclor 1254)-induced oxidative damage in Leydig cells. Free Radic Res 2005;39:1259–72. doi: 10.1080/10715760500308154
- Othman N, Ismail Z, Selamat MI, Sheikh Abdul Kadir SH, Shibraumalisi NA. A review of polychlorinated biphenyls (PCBs) pollution in the air: where and how much are we exposed to? Int J Environ Res Public Health 2022;19(21):13923. doi: 10.3390/ijerph192113923
- Ateşşahin A, Türk G, Yilmaz S, Sönmez M, Sakin F, Ceribasi AO. Modulatory effects of lycopene and ellagic acid on reproductive dysfunction induced by polychlorinated biphenyl (Aroclor 1254) in male rats. Basic Clin Pharmacol Toxicol 2010;106:479–89. doi: 10.1111/j.1742-7843.2009.00529.x
- Senthil kumar J, Banudevi S, Sharmila M, Murugesan P, Srinivasan N, Balasubramanian K, Aruldhas MM, Arunakaran J. Effects of Vitamin C and E on PCB (Aroclor 1254) induced oxidative stress, androgen binding protein and lactate in rat Sertoli cells. Reprod Toxicol 2004;19:201–8. doi: 10.1016/j.reprotox.2004.08.001
- Güleş Ö, Eren Ü. Protective role of alpha lipoic acid against polychlorobiphenyl (Aroclor 1254)-induced testicular toxicity in rats. Rom J Morphol Embryol 2016;57:451–9. PMID: 27516018
- Elumalai P, Krishnamoorthy G, Selvakumar K, Arunkumar R, Venkataraman P, Arunakaran J. Studies on the protective role of lycopene against polychlorinated biphenyls (Aroclor 1254)-induced changes in StAR protein and cytochrome P450 scc enzyme expression on Leydig cells of adult rats. Reprod Toxicol 2009;27:41–5. doi: 10.1016/j.reprotox.2008.11.053
- Kimbrough RD. Polychlorinated biphenyls (PCBs) and human health: an update. Crit Rev Toxicol 1995;25:133–63. doi: 10.3109/10408449509021611
- Krishnamoorthy G, Venkataraman P, Arunkumar A, Vignesh RC, Aruldhas MM, Arunakaran J. Ameliorative effect of vitamins (alpha-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm. Reprod Toxicol 2007;23:239–45. doi: 10.1016/j.reprotox.2006.12.004
- Sugantha Priya E, Sathish Kumar T, Balaji S, Bavithra S, Raja Singh P, Sakthivel D, Ravi Sankar B, Arunakaran J. Lactational exposure effect of polychlorinated biphenyl on rat Sertoli cell markers and functional regulators in prepuberal and puberal F(1) offspring. J Endocrinol Invest 2017;40:91–100. doi: 10.1007/s40618-016-0539-0
- Barchielli G, Capperucci A, Tanini D. The role of selenium in pathologies: an updated review. Antioxidants (Basel) 2022;11(2):251. doi: 10.3390/antiox11020251
- Keshta AT, Fathallah AM, Attia YA, Salem EA, Watad SH. Ameliorative effect of selenium nanoparticles on testicular toxicity induced by cisplatin in adult male rats. Food Chem Toxicol 2023;179:113979. doi: 10.1016/j.fct.2023.113979
- Akerboom TP, Sies H. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 1981;77:373–82. doi: 10.1016/s0076-6879(81)77050-2
- Erkekoglu P, Zeybek ND, Giray B, Asan E, Arnaud J, Hincal F. Reproductive toxicity of di(2-ethylhexyl) phthalate in selenium-supplemented and selenium-deficient rats. Drug Chem Toxicol 2011;34:379–89. doi: 10.3109/01480545.2010.547499
- Zhao H, Zhu Y, Zhao Y, Wang T, Li H, Yang J, Cheng X, Wang J, Wang J. Alleviating effects of selenium on fluoride-induced testosterone synthesis disorder and reproduction toxicity in rats. Ecotoxicol Environ Saf 2022;247:114249. doi: 10.1016/j.ecoenv.2022.114249
- Kiełczykowska M, Kocot J, Paździor M, Musik I. Selenium – a fascinating antioxidant of protective properties. Adv Clin Exp Med 2018;27:245–55. doi: 10.17219/acem/67222
- Erkekoglu P, Giray BK, Kizilgun M, Hininger-Favier I, Rachidi W, Roussel AM, Favier A, Hincal F. Thyroidal effects of di-(2-ethylhexyl) phthalate in rats of different selenium status. J Environ Pathol Toxicol Oncol 2012;31:143–53. doi: 10.1615/JEnvironPatholToxicolOncol.v31.i2.60
- Kaur P, Bansal MP. Effect of selenium-induced oxidative stress on the cell kinetics in testis and reproductive ability of male mice. Nutrition 2005;21:351–7. doi: 10.1016/j.nut.2004.05.028
- Murugesan P, Muthusamy T, Balasubramanian K, Arunakaran J. Polychlorinated biphenyl (Aroclor 1254) inhibits testosterone biosynthesis and antioxidant enzymes in cultured rat Leydig cells. Reprod Toxicol 2008;25:447–54. doi: 10.1016/j.reprotox.2008.04.003
- Behne D, Weiler H, Kyriakopoulos A. Effects of selenium deficiency on testicular morphology and function in rats. J Reprod Fertil 1996;106:291–7. doi: 10.1530/jrf.0.1060291
- Basini G, Tamanini C. Selenium stimulates estradiol production in bovine granulosa cells: possible involvement of nitric oxide. Domest Anim Endocrinol 2000;18:1–17. doi: 10.1016/s0739-7240(99)00059-4
- Khoshbakht S, Motejaded F, Karimi S, Jalilvand N, Ebrahimzadeh-Bideskan A. Protective effects of selenium on electromagnetic field-induced apoptosis, aromatase P450 activity, and leptin receptor expression in rat testis. Iran J Basic Med Sci 2021;24:322–30. doi: 10.22038/ijbms.2021.45358.10554
- Gaschler MM, Stockwell BR. Lipid peroxidation in cell death. Biochem Biophys Res Commun 2017;482:419–25. doi: 10.1016/j.bbrc.2016.10.086
- Avery SV. Molecular targets of oxidative stress. Biochem J 2011;434:201–10. doi: 10.1042/BJ20101695
- Kim JM, Ghosh SR, Weil AC, Zirkin BR. Caspase-3 and caspase-activated deoxyribonuclease are associated with testicular germ cell apoptosis resulting from reduced intratesticular testosterone. Endocrinology 2001;142:3809–16. doi: 10.1210/endo.142.9.8375
- Burns TF, El-Deiry WS. The p53 pathway and apoptosis. J Cell Physiol 1999;181:231–9. doi: 10.1002/(SICI)1097-4652(199911)181:2<;231::AID-JCP5>3.0.CO;2-L
- Ahmadi K, Roshan-Milani S, Asgharzadeh F, Pourjabali M, Fard AA. In vitro and in vivo pretreatment with selenium mitigates tetrahydrocannabinol-induced testicular cell apoptosis: the role of AKT and p53 pathways. Biol Trace Elem Res 2021;199:2278–87. doi: 10.1007/s12011-020-02322-5
- Hsu PC, Pan MH, Li LA, Chen CJ, Tsai SS, Guo YL. Exposure in utero to 2,2',3,3',4,6'-hexachlorobiphenyl (PCB 132) impairs sperm function and alters testicular apoptosis-related gene expression in rat offspring. Toxicol Appl Pharmacol 2007;221:68–75. doi: 10.1016/j.taap.2007.01.027
- Attia SM, Ahmad SF, Okash RM, Bakheet SA. Aroclor 1254-induced genotoxicity in male gonads through oxidatively damaged DNA and inhibition of DNA repair gene expression. Mutagenesis 2014;29:379–84. doi: 10.1093/mutage/geu029
Language: English
Page range: 102 - 113
Submitted on: Jul 1, 2025
Accepted on: May 1, 2026
Published on: Jun 30, 2026
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Keywords:
Related subjects:
© 2026 Pınar Erkekoğlu, Aylin Balcı-Özyurt, Naciye Dilara Zeybek, Hülya Tezel-Yalçın, Ünzile Yaman, Gizem Yildiztekin, Sevtap Aydın-Dilsiz, Murat Kızılgün, Evin İşcan, Tuğçe Batur, Mehmet Öztürk, Oğuz Han Edebal, Nurşen Başaran, Belma Koçer-Gumusel, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.