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Chlorogenic acid protects against cisplatin-induced testicular damage: a biochemical and histological study Cover

Chlorogenic acid protects against cisplatin-induced testicular damage: a biochemical and histological study

Archives of Industrial Hygiene and Toxicology
Volume 76 (2025): Issue 2 (June 2025)
By:
Open Access
|Jun 2025

Figures & Tables

Figure 1

Effects of CHA (1.5 and 3 mg/kg) on OS parameters in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. **p<0.01 and ***p<0.001 – significant difference from control; #p<0.05, ##p<0.01, and ###p<0.001 – significant difference from the CIS alone group; ++p<0.01 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; GPx – glutathione peroxidase; GSH – reduced glutathione; MDA – malondialdehyde; OS – oxidative stress; SEM – standard error of the mean; SOD – superoxide dismutase
Effects of CHA (1.5 and 3 mg/kg) on OS parameters in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. **p<0.01 and ***p<0.001 – significant difference from control; #p<0.05, ##p<0.01, and ###p<0.001 – significant difference from the CIS alone group; ++p<0.01 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; GPx – glutathione peroxidase; GSH – reduced glutathione; MDA – malondialdehyde; OS – oxidative stress; SEM – standard error of the mean; SOD – superoxide dismutase

Figure 2

Effects of CHA (1.5 and 3 mg/kg) on inflammation parameters in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. ***p<0.001 – significant difference from control; #p<0.05, ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; ++p<0.01 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; IL-6 – interleukin-6; MPO – myeloperoxidase; NF-κB – nuclear factor kappa B; SEM – standard error of the mean
Effects of CHA (1.5 and 3 mg/kg) on inflammation parameters in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. ***p<0.001 – significant difference from control; #p<0.05, ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; ++p<0.01 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; IL-6 – interleukin-6; MPO – myeloperoxidase; NF-κB – nuclear factor kappa B; SEM – standard error of the mean

Figure 3

Effects of CHA (1.5 and 3 mg/kg) on ERS and apoptosis parameters in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. *p<0.05, **p<0.01 and ***p<0.001 – significant difference from control; ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; ++p<0.01 and +++p<0.001 – significant difference from the CIS+CHA (1.5 mg/kg) g roup. ATF6 – activating transcription factor 6; CASP3 – cleaved caspase-3; CHA – chlorogenic acid; CIS – cisplatin; DDIT3 – DNA damage-inducible transcript 3; ERS – endoplasmic reticulum stress; HSPA5 – heat shock protein family A (HSP70) member 5; SEM – standard error of the mean
Effects of CHA (1.5 and 3 mg/kg) on ERS and apoptosis parameters in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. *p<0.05, **p<0.01 and ***p<0.001 – significant difference from control; ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; ++p<0.01 and +++p<0.001 – significant difference from the CIS+CHA (1.5 mg/kg) g roup. ATF6 – activating transcription factor 6; CASP3 – cleaved caspase-3; CHA – chlorogenic acid; CIS – cisplatin; DDIT3 – DNA damage-inducible transcript 3; ERS – endoplasmic reticulum stress; HSPA5 – heat shock protein family A (HSP70) member 5; SEM – standard error of the mean

Figure 4

Effects of CHA (1.5 and 3 mg/kg) on the Nrf2/HO-1 pathway in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. ***p<0.001 – significant difference from control; ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; +p<0.05 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; HO-1 – haem oxygenase-1; Nrf2 – nuclear factor erythroid 2-related factor 2; SEM – standard error of the mean
Effects of CHA (1.5 and 3 mg/kg) on the Nrf2/HO-1 pathway in a CIS-induced testicular toxicity rat model. Data are presented as means ± SEM. ***p<0.001 – significant difference from control; ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; +p<0.05 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; HO-1 – haem oxygenase-1; Nrf2 – nuclear factor erythroid 2-related factor 2; SEM – standard error of the mean

Figure 5

Effects of CHA (1.5 and 3 mg/kg) on testicular tissue architecture in CIS-treated rats (200×). A) control; B) CHA alone (3 mg/kg); C) CIS (5 mg/kg); D) CIS+CHA (1.5 mg/kg); E) CIS+CHA (3 mg/kg); F) Mean Johnsen scores for all groups. Data are presented as means ± SEM. ***p<0.001 – significant difference from control; ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; +++p<0.001 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; SEM – standard error of the mean
Effects of CHA (1.5 and 3 mg/kg) on testicular tissue architecture in CIS-treated rats (200×). A) control; B) CHA alone (3 mg/kg); C) CIS (5 mg/kg); D) CIS+CHA (1.5 mg/kg); E) CIS+CHA (3 mg/kg); F) Mean Johnsen scores for all groups. Data are presented as means ± SEM. ***p<0.001 – significant difference from control; ##p<0.01 and ###p<0.001 – significant difference from the CIS alone group; +++p<0.001 – significant difference from the CIS+CHA (1.5 mg/kg) group. CHA – chlorogenic acid; CIS – cisplatin; SEM – standard error of the mean
DOI: https://doi.org/10.2478/aiht-2025-76-3990 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 130 - 137
Submitted on: Apr 1, 2025
Accepted on: Jun 1, 2025
Published on: Jun 30, 2025
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
apoptosis,
endoplasmic reticulum stress,
HO-1,
inflammation,
Nrf2,
oxidative stress
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2025 Elif Ayazoğlu Demir, Selim Demir, Sevdegül Aydın Mungan, Nihal Türkmen Alemdar, Ahmet Menteşe, Yüksel Aliyazıcıoğlu, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

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