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Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells Cover

Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells

Archives of Industrial Hygiene and Toxicology
Volume 75 (2024): Issue 2 (June 2024)
By: Ana Valenta Šobot,  Marijana Janić,  Iva Popović,  Tamara Lazarević-Pašti,  Tatjana Momić,  Aleksandar Krstić and  Jelena Filipović Tričković  
Open Access
|Jun 2024

Figures & Tables

Figure 1

Scavenging properties of aqueous sage extract determined with the DPPH• (A) and ABTS•+ assay (B) and expressed as a percentage of inhibition compared to control
Scavenging properties of aqueous sage extract determined with the DPPH• (A) and ABTS•+ assay (B) and expressed as a percentage of inhibition compared to control

Figure 2

Viability of peripheral blood mononuclear cells treated with different concentrations (0.075–2.25 mg/mL) of aqueous sage extract for 24 h compared to untreated control. **P<0.01; ***P<0.001
Viability of peripheral blood mononuclear cells treated with different concentrations (0.075–2.25 mg/mL) of aqueous sage extract for 24 h compared to untreated control. **P<0.01; ***P<0.001

Figure 3

Glutathione reductase activity (mU/mL) in untreated control whole blood samples and those treated with increasing concentrations of aqueous sage extract. **P<0.01; ***P<0.001
Glutathione reductase activity (mU/mL) in untreated control whole blood samples and those treated with increasing concentrations of aqueous sage extract. **P<0.01; ***P<0.001

Figure 4

Prooxidant-antioxidant balance in untreated (control) peripheral blood mononuclear cells vs those treated with H2O2, aqueous sage extract, and their combination (presented as a percentage of untreated control). *P<0.05; ***P<0.001 – treatments vs. untreated control. ###P<0.001 – sage pre-treatments vs. H2O2 treatment
Prooxidant-antioxidant balance in untreated (control) peripheral blood mononuclear cells vs those treated with H2O2, aqueous sage extract, and their combination (presented as a percentage of untreated control). *P<0.05; ***P<0.001 – treatments vs. untreated control. ###P<0.001 – sage pre-treatments vs. H2O2 treatment

Figure 5

Micronucleus frequency in peripheral blood cells: untreated controls, H2O2-treated, sage extract-treated, and pre-treated with sage extract followed by H2O2 (presented as a percentage of untreated control). *P<0.05, ***P<0.001 – treated vs. control cells. ###P<0.001 – sage extract pre-treatment vs. H2O2 treatment
Micronucleus frequency in peripheral blood cells: untreated controls, H2O2-treated, sage extract-treated, and pre-treated with sage extract followed by H2O2 (presented as a percentage of untreated control). *P<0.05, ***P<0.001 – treated vs. control cells. ###P<0.001 – sage extract pre-treatment vs. H2O2 treatment

Figure 6

Cytokinesis-block proliferation indices (CBPI) of peripheral blood cells: untreated controls, H2O2-treated, sage extract-treated, and pre-treated with sage extract followed by H2O2 (presented as a percentage of untreated control). *P<0.05, ** P<0.01 – treated cells vs. control cells. #P<0.05 – sage extract pre-treatment vs. H2O2 treatment
Cytokinesis-block proliferation indices (CBPI) of peripheral blood cells: untreated controls, H2O2-treated, sage extract-treated, and pre-treated with sage extract followed by H2O2 (presented as a percentage of untreated control). *P<0.05, ** P<0.01 – treated cells vs. control cells. #P<0.05 – sage extract pre-treatment vs. H2O2 treatment

Figure 7

Pro-inflammatory cytokine levels, IL-1β (A) and TNF-α (B), in PHA-stimulated peripheral blood cells treated with aqueous sage extract in different concentrations. * P <0.05, ***P<0.001 – treated cells vs. control cells
Pro-inflammatory cytokine levels, IL-1β (A) and TNF-α (B), in PHA-stimulated peripheral blood cells treated with aqueous sage extract in different concentrations. * P <0.05, ***P<0.001 – treated cells vs. control cells

AChE inhibition expressed as percentage of inhibited control activity

Extract concentration (mg/mL)AChE inhibition (%)
0.0750
0.1250
0.62516
1.2531
1041

Relative percentage of identified compounds in the aqueous sage extract

No.ComponentsRRIRTPeak area (%)
1α-terpineol11965.140.93
2β-thujone11035.930.89
3Camphor11436.3615.47
4endo-Borneol11676.572.89
5α-Bornyl acetate128810.007.58
6Acetophenone106510.307.37
72,4 Di-tert butyl phenol151911.137.05
8Palmitic acid196815.7118.71
9Linoleic acid217217.108.16
10Monopalmitin249817.653.74
113-hydroxypropyl palmitate236118.9611.89
12α-glycerol monostearate280620.0013.39
DOI: https://doi.org/10.2478/aiht-2024-75-3836 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 137 - 146
Submitted on: Mar 1, 2024
|
Accepted on: May 1, 2024
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Published on: Jun 29, 2024
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
AChE inhibition,
antigenotoxic effects,
anti-inflammatory effects,
antioxidant,
Salvia officinalis L
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2024 Ana Valenta Šobot, Marijana Janić, Iva Popović, Tamara Lazarević-Pašti, Tatjana Momić, Aleksandar Krstić, Jelena Filipović Tričković, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

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