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A new strategy to achieve high antimicrobial activity: green synthesised silver nanoparticle formulations with Galium aparine and Helichrysum arenarium Cover

A new strategy to achieve high antimicrobial activity: green synthesised silver nanoparticle formulations with Galium aparine and Helichrysum arenarium

Open Access
|Jun 2023

Figures & Tables

Figure 1

UV–Vis absorption spectra of a) G-AgNPs and b) H-AgNPs with different incubation times (0–240 min)
UV–Vis absorption spectra of a) G-AgNPs and b) H-AgNPs with different incubation times (0–240 min)

Figure 2

Particle size distribution of a) G-AgNPs and b) H-AgNPs
Particle size distribution of a) G-AgNPs and b) H-AgNPs

Figure 3

Zeta potential of a) G-AgNPs and b) H-AgNPs
Zeta potential of a) G-AgNPs and b) H-AgNPs

Figure 4

FT-IR spectrum of a) G. aparine, b) G-AgNPs, c) H. arenarium, and d) H-AgNPs
FT-IR spectrum of a) G. aparine, b) G-AgNPs, c) H. arenarium, and d) H-AgNPs

Figure 5

2-D Atomic force microscope images of a) G-AgNPs and b) H-AgNPs. Scan scale: 1×1 µm
2-D Atomic force microscope images of a) G-AgNPs and b) H-AgNPs. Scan scale: 1×1 µm

Figure 6

Scanning electron microscope images of a) G-AgNPs and b) H-AgNPs at 200,000× magnification
Scanning electron microscope images of a) G-AgNPs and b) H-AgNPs at 200,000× magnification

Table 3

Petri dish images obtained by standard plate counting related to respective MICs. The dilution coefficient for E. coli is 102. There is no dilution for other microorganism. MIC – minimum inhibitory concentration
Petri dish images obtained by standard plate counting related to respective MICs. The dilution coefficient for E. coli is 102. There is no dilution for other microorganism. MIC – minimum inhibitory concentration

Physicochemical properties of H-AgNPs and G-AgNPs

NPsParticle Size (nm)Zeta Potential (mV)Polydispersity Index
H-AgNP23.9±1.0−21.3±2.70.285±0.034
G-AgNP52.0±10.9−17.9±0.90.280±0.032

MBC/MFC and MIC values of samples according to the broth microdilution method

Test sampleAgNP concentrations used in nanoformulations (μg/mL)MIC μ/mL) by microorganismsMBC/MFC μ/mL) by microorganisms
E. coliS. aureusA. flavusC. albicansE. coliS. aureusA. flavusC. albicans
G-AgNPs 62.52000125
H-AgNPs 125500
NF-11000 G-AgNP + 1000 H-AgNP31.25*125500125250
NF-2500 G-AgNP + 1500 H-AgNP31.25*62.5500125125
NF-31500 G-AgNP + 500 H-AgNP31.25*62.5500125125
DOI: https://doi.org/10.2478/aiht-2023-74-3684 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Language: English, Croatian, Slovenian
Page range: 90 - 98
Submitted on: Oct 1, 2022
Accepted on: Jun 1, 2023
Published on: Jun 26, 2023
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2023 Cilem Ozdemir, Merve Gencer, Irem Coksu, Tulin Ozbek, Serap Derman, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.