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A new strategy to achieve high antimicrobial activity: green synthesised silver nanoparticle formulations with Galium aparine and Helichrysum arenarium Cover

A new strategy to achieve high antimicrobial activity: green synthesised silver nanoparticle formulations with Galium aparine and Helichrysum arenarium

Open Access
|Jun 2023

Figures & Tables

Figure 1

UV–Vis absorption spectra of a) G-AgNPs and b) H-AgNPs with different incubation times (0–240 min)

Figure 2

Particle size distribution of a) G-AgNPs and b) H-AgNPs

Figure 3

Zeta potential of a) G-AgNPs and b) H-AgNPs

Figure 4

FT-IR spectrum of a) G. aparine, b) G-AgNPs, c) H. arenarium, and d) H-AgNPs

Figure 5

2-D Atomic force microscope images of a) G-AgNPs and b) H-AgNPs. Scan scale: 1×1 µm

Figure 6

Scanning electron microscope images of a) G-AgNPs and b) H-AgNPs at 200,000× magnification

Table 3

Petri dish images obtained by standard plate counting related to respective MICs. The dilution coefficient for E. coli is 102. There is no dilution for other microorganism. MIC – minimum inhibitory concentration

Physicochemical properties of H-AgNPs and G-AgNPs

NPsParticle Size (nm)Zeta Potential (mV)Polydispersity Index
H-AgNP23.9±1.0−21.3±2.70.285±0.034
G-AgNP52.0±10.9−17.9±0.90.280±0.032

MBC/MFC and MIC values of samples according to the broth microdilution method

Test sampleAgNP concentrations used in nanoformulations (μg/mL)MIC μ/mL) by microorganismsMBC/MFC μ/mL) by microorganisms
E. coliS. aureusA. flavusC. albicansE. coliS. aureusA. flavusC. albicans
G-AgNPs 62.52000125
H-AgNPs 125500
NF-11000 G-AgNP + 1000 H-AgNP31.25*125500125250
NF-2500 G-AgNP + 1500 H-AgNP31.25*62.5500125125
NF-31500 G-AgNP + 500 H-AgNP31.25*62.5500125125
DOI: https://doi.org/10.2478/aiht-2023-74-3684 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Language: English, Croatian, Slovenian
Page range: 90 - 98
Submitted on: Oct 1, 2022
Accepted on: Jun 1, 2023
Published on: Jun 26, 2023
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2023 Cilem Ozdemir, Merve Gencer, Irem Coksu, Tulin Ozbek, Serap Derman, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.