Kidney cell DNA damage caused by combined exposure to volatile anaesthetics and 1 Gy or 2 Gy radiotherapy dose in vivo

	Control	Halothane (2.4 % v/v)	Sevoflurane (2.4 % v/v)	Isoflurane (1.7 % v/v)
Non-irradiated
Time points	0 h	0 h	0 h	0 h
	2 h	2 h	2 h	2 h
	6 h	6 h	6 h	6 h
	24 h	24 h	24 h	24 h
Irradiated with 1 Gy
Time points	2 h	2 h	2 h	2 h
	0 h	0 h	0 h	0 h
	6 h	6 h	6 h	6 h
	24 h	24 h	24 h	24 h
Irradiated with 2 Gy
Time points	0 h	0 h	0 h	0 h
	2 h	2 h	2 h	2 h
	6 h	6 h	6 h	6 h
	24 h	24 h	24 h	24 h

Tail length and tail intensity in kidney cells of control (C) Swiss albino mice and mice subjected to two-hour anaesthesia with halothane (H), sevoflurane (S), or isoflurane (I) at 0, 2, 6, and 24 h after anaesthesia (based on the total score of 200 comets per group (40 comets per animal)

Tail length and tail intensity in kidney cells of Swiss albino mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or gamma-irradiation (⁶⁰Co) of 1 Gy 0, 2, 6, and 24 h after irradiation [(based on the total score of 200 comets per group (40 comets per animal)]

Table 2

Cellular DNA repair index (percentage of repair) of tail length and tail intensity in non-irradiated mice and mice exposed to halothane (H), sevoflurane (S), or isoflurane (I) and/or 1 Gy or 2 Gy 2, 6, and 24 h from irradiation

	Tail length			Tail intensity
	2 h	6 h	24 h	2 h	6 h	24 h
Non-irradiated
Halothane	-17.86	-12.11	3.56	10.32	28.42	-6.44
Sevoflurane	-2.56	16.14	12.39	-15.71	-0.65	-10.11
Isoflurane	-10.71	-3.42	-15.28	26.77	28.12	-15.62
Irradiated with 1 Gy
1 Gy	3.76	12.01	-18.10	24.20	-58.95	-25.08
Halothane + 1Gy	-9.78	-1.46	-7.86	15.28	2.54	-7.38
Sevoflurane + 1Gy	16.06	-2.07	1.76	3.35	11.02	-10.80
Iisoflurane + 1Gy	14.92	15.91	7.34	43.96	4.92	36.08
Irradiated with 2 Gy
2 Gy	-28.19	-17.81	-11.22	-29.75	-90.54	-40.43
Halothane + 2Gy	-16.54	-19.53	5.97	25.87	18.70	27.56
Sevoflurane + 2Gy	1.44	1.59	3.09	-20.68	-7.65	24.16
Isoflurane + 2Gy	15.54	2.52	-5.55	39.13	-77.69	-87.18

Table 3

Statistical differences (Mann–Whitney U test; p<0.05), in tail length (TL, μm) and tail intensity (TI, % of DNA in comet tail) in kidney cells of Swiss albino mice treated with halothane, sevoflurane, or isoflurane alone or in combination with 1 or 2 Gy gamma-irradiation (⁶⁰Co) compared to corresponding controls at the same time point

Non-irradiated
	Exposure	Halothane		Sevoflurane		Isoflurane
Time points		TL	TI	TL	TI	TL	TI
0 h		NS	<0.001	<0.001	<0.001	<0.001	0.004
2 h		<0.001	<0.001	0.025	<0.001	NS	NS
6 h		<0.001	<0.001	<0.001	0.01	0.021	NS
24 h		<0.001	<0.001	NS	<0.001	NS	<0.001
Irradiated 1 Gy
Exposure		Halothane + 1 Gy		Sevoflurane + 1 Gy		Isoflurane + 1 Gy
Time points		TL	TI	TL	TI	TL	TI
0 h		<0.001	<0.001	NS	NS	NS	0.014
2 h		<0.001	<0.001	<0.001	NS	<0.001	NS
6 h		<0.001	NS	<0.001	0.006	NS	NS
24 h		<0.001	0.004	0.001	NS	<0.001	0.003
Irradiated 2 Gy
	Exposure	Halothane + 2 Gy		Sevoflurane + 2 Gy		Isoflurane + 2 Gy
Time points		TL	TI	TL	TI	TL	TI
0 h		<0.001	<0.001	<0.001	<0.001	<0.001	NS
2 h		<0.001	0.002	NS	<0.001	<0.001	0.002
6 h		<0.001	NS	<0.001	NS	0.02	NS
24 h		<0.001	<0.001	<0.001	NS	<0.001	<0.001

[i] NS – difference is not statistically significant

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DOI: https://doi.org/10.2478/aiht-2022-73-3600 | Journal eISSN: 1848-6312 (formerly 0004-1254) | Journal ISSN: 0004-1254

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Language: English, Croatian

Page range: 62 - 70

Submitted on: Oct 1, 2021

Accepted on: Mar 1, 2022

Published on: Apr 7, 2022

Published by: Institute for Medical Research and Occupational Health

In partnership with: Paradigm Publishing Services

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© 2022 Vesna Benković, Nada Oršolić, Anica Horvat Knežević, Nikola Borojević, Gordana Brozović, Mirta Milić, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 73 (2022): Issue 1 (March 2022)

Kidney cell DNA damage caused by combined exposure to volatile anaesthetics and 1 Gy or 2 Gy radiotherapy dose in vivo

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