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Primary DNA damage measured in SH-SY5Y cells using the alkaline comet assay after treatment with 6_25 μmol/L (2_02 mg/L) LSD, 3_13 μmol/L (0_76 mg/L) PCP, and in respective negative and positive controls
| Sample | Negative control (untreated cells) | LSD | PCP | Positive control (H2O2) | ||||
|---|---|---|---|---|---|---|---|---|
| Descriptor | Tail DNA% | Tail length (μm) | Tail DNA% | Tail length (μm) | Tail DNA% | Tail length (μm) | Tail DNA% | Tail length (μm) |
| Mean | 1.57 | 13.55 | 3.14* | 15.46* | 2.30 | 15.65* | 8.33* | 15.84* |
| SE | 0.14 | 0.26 | 0.33 | 0.32 | 0.25 | 0.27 | 0.66 | 0.39 |
| SD | 2.43 | 4.53 | 5.68 | 5.51 | 4.27 | 4.60 | 11.51 | 6.84 |
| Median | 0.22 | 12.50 | 0.62 | 13.75 | 0.21 | 14.58 | 2.87 | 13.75 |
| Minimum | 0 | 6.25 | 0 | 7.50 | 0 | 7.92 | 0 | 5.00 |
| Maximum | 11.97 | 28.33 | 45.77 | 54.17 | 29.28 | 37.08 | 63.81 | 45.83 |
Experimental schedule
| Experimental group | LSD (mg/L) | PCP (mg/L) |
|---|---|---|
| 0.39 μmol/L | 0.13 | 0.09 |
| 0.78 μmol/L | 0.25 | 0.19 |
| 1.56 μmol/L | 0.50 | 0.38 |
| 3.13 μmol/L | 1.01 | 0.76 |
| 6.25 μmol/L | 2.02 | 1.52 |
| 12.50 μmol/L | 4.04 | 3.04 |
| 25 μmol/L | 8.09 | 6.08 |
| 50 μmol/L | 16.17 | 12.17 |
| 100 μmol/L | 32.34 | 24.34 |
| Control | untreated cells | |
Viability of SH-SY5Y cells exposed to LSD and PCP in concentrations of 0_39–100 μmol/L for 24 h determined with the MTS assay
| Experimental group | Viable cells (mean % ±SD) | |
|---|---|---|
| LSD | PCP | |
| Control | 99.67±3.74 | 99.20±4.42 |
| 0.39 μmol/L | 97.77±7.94 | 92.65±6.25 |
| 0.78 μmol/L | 93.68±8.35* | 90.05±7.07 |
| 1.56 μmol/L | 90.26±7.52* | 86.54±11.08* |
| 3.13 μmol/L | 90.67±5.95* | 84.17±9.03* |
| 6.25 μmol/L | 88.06±7.44* | 78.10±5.49* |
| 12.5 μmol/L | 94.18±8.18 | 76.28±10.10* |
| 25 μmol/L | 100.17±7.29 | 75.57±9.15* |
| 50 μmol/L | 101.82±6.77 | 71.02±13.29* |
| 100 μmol/L | 98.09±7.63 | 72.80±8.85* |