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Ortho-substituted PCB 153: effects in CHO-K1 cells Cover

Ortho-substituted PCB 153: effects in CHO-K1 cells

Archives of Industrial Hygiene and Toxicology
Volume 72 (2021): Issue 4 (December 2021)
By: Marina Miletić,  Teuta Murati,  Branimir Šimić,  Nina Bilandžić,  Anamaria Brozović and  Ivana Kmetič  
Open Access
|Dec 2021

Figures & Tables

Figure 1

The effects of 10–100 μmol/L PCB 153 on the viability and proliferation of CHO-K1 cells after 6, 24, 48, and 72 h of exposure determined with the Trypan Blue (A), Neutral Red (B), Kenacid Blue (C), and MTT assays (D). Data are presented as mean percentages of control (±SEM). Statistical significance vs control: ap<0.001; bp<0.005; cp<0.01; dp<0.025; ep<0.05

Figure 2

CHO-K1 cell death after 6 h of exposure to 10–100 μmol/L PCB 153. (A) representative dot plot profiles of control and PCB-treated cells; (B) distribution of live, total apoptotic, and dead cells (mean±SEM); (C) distribution of early apoptotic and late apoptotic/dead cells relative to the total number of cells (mean±SEM). Statistical significance vs respective controls: ap<0.001; bp<0.005; cp<0.01; dp<0.025

Figure 3

CHO-K1 cell death after 48 h of exposure to 10–100 μmol/L PCB 153. (A) representative dot plot profiles of control and PCB-treated cells; (B) distribution of live, total apoptotic, and dead cells (mean±SEM); (C) distribution of early apoptotic and late apoptotic/dead cells relative to the total number of cells (mean±SEM)

Figure 4

CHO-K1 cell cycle analysis after exposure to 50 μmol/L PCB 153. (A) representative flow cytometric histograms of cell cycle progression after 6 and 48-hour exposure (B) Distribution (percentage) of exposed cells across the cell cycle phases over 72 h

Figure 5

Effects of 10–100 μmol/L PCB 153 on ROS formation in CHO-K1 cells. Data are presented as mean percentage of control (±SEM). *p<0.05 vs control

Inhibition concentrations (μmol/L) determined with four different cytotoxicity assays after 6, 24, 48, and 72 h of CHO-K1 cell exposure to PCB 153

PCB 153 (μmol/L)
6 h24 h48 h72 h
EndpointIC20IC50IC80IC20IC50IC80IC20IC50IC80IC20IC50IC80
TB32.766.392.623.364.5ND28.566.198.320.855.588.6
NR61.5NDND22.665.9ND34.065.289.321.751.078.3
KB61.0NDND77.4NDND73.095.9ND85.2NDND
MTT19.758.498.667.8NDND63.4NDND34.886.7ND
DOI: https://doi.org/10.2478/aiht-2021-72-3588 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 326 - 332
Submitted on: Sep 1, 2021
|
Accepted on: Nov 1, 2021
|
Published on: Dec 30, 2021
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
cell cycle progression,
cell death,
cytotoxicity,
polychlorinated biphenyls,
ROS
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2021 Marina Miletić, Teuta Murati, Branimir Šimić, Nina Bilandžić, Anamaria Brozović, Ivana Kmetič, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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