Have a personal or library account? Click to login
Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells Cover

Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells

Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 4 (December 2020)
By: Ana Valenta Šobot,  Dunja Drakulić,  Gordana Joksić,  Jadranka Miletić Vukajlović,  Jasmina Savić,  Jelena Potočnik and  Jelena Filipović Tričković  
Open Access
|Dec 2020

Figures & Tables

Figure 1

Particle size distribution of dispersed particles in (a) yellow gentian root powder; (b) lyophilisate; and (c) dissolved lyophilisate (pre-filtrate). Scale bar – 100 μm
Particle size distribution of dispersed particles in (a) yellow gentian root powder; (b) lyophilisate; and (c) dissolved lyophilisate (pre-filtrate). Scale bar – 100 μm

Figure 2

UPLC chromatograms of yellow gentian root extract in ethanol solution exported at 254 nm (a); 300 nm (b); 270 nm (c); and 240 nm (d). 1 – loganic acid; 2 – swertiamarin; 3 – overlapping gentiopicroside and sweroside peaks
UPLC chromatograms of yellow gentian root extract in ethanol solution exported at 254 nm (a); 300 nm (b); 270 nm (c); and 240 nm (d). 1 – loganic acid; 2 – swertiamarin; 3 – overlapping gentiopicroside and sweroside peaks

Figure 3

Cell viability expressed as percentage of control (a) and percentage of chromosome breaks (b) at different concentrations of yellow gentian root extract after 48 and 72 h treatment. *p<0.05, **p<0.01, ***p<0.001
Cell viability expressed as percentage of control (a) and percentage of chromosome breaks (b) at different concentrations of yellow gentian root extract after 48 and 72 h treatment. *p<0.05, **p<0.01, ***p<0.001

Figure 4

Photomicrograph of metaphase spreads after 72 h treatment with 2 mg/mL of yellow gentian root extract. Arrows mark chromatid break (a) and radial figure between two homologous chromosomes (17q) an indicator of homologous recombination - a mechanism of template-dependent DNA DSBs repair (b)
Photomicrograph of metaphase spreads after 72 h treatment with 2 mg/mL of yellow gentian root extract. Arrows mark chromatid break (a) and radial figure between two homologous chromosomes (17q) an indicator of homologous recombination - a mechanism of template-dependent DNA DSBs repair (b)

Figure 5

Graph (a) and photomicrograph (b-e) of YGRE-induced DNA damage established with the comet assay. b – control; c – 0.5 mg/mL; d – 1 mg/mL; e – 2 mg/mL; YGRE – yellow gentian root extract. Scale bar – 10 μm. ***p<0.001
Graph (a) and photomicrograph (b-e) of YGRE-induced DNA damage established with the comet assay. b – control; c – 0.5 mg/mL; d – 1 mg/mL; e – 2 mg/mL; YGRE – yellow gentian root extract. Scale bar – 10 μm. ***p<0.001

Figure 6

PAB levels presented as arbitrary HK units (a) and LPP levels expressed as nmol/mg of proteins (b) after treatment with increasing concentrations of yellow gentian root extract. ***p<0.001
PAB levels presented as arbitrary HK units (a) and LPP levels expressed as nmol/mg of proteins (b) after treatment with increasing concentrations of yellow gentian root extract. ***p<0.001

Gradient condition details used for UPLC separation_

time (min)% B
0 → 210 → 28
2 → 3.528 → 30
3.5 → 530 → 55
5 → 5.155 → 10
5.1 → 610 →
DOI: https://doi.org/10.2478/aiht-2020-71-3476 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Journal RSS Feed
Language: English, Croatian, Slovenian
Page range: 320 - 328
Submitted on: Aug 1, 2020
|
Accepted on: Oct 1, 2020
|
Published on: Dec 31, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
cytotoxicity,
genotoxicity,
Gentiana lutea L.,
homologous recombination,
redox parameters
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2020 Ana Valenta Šobot, Dunja Drakulić, Gordana Joksić, Jadranka Miletić Vukajlović, Jasmina Savić, Jelena Potočnik, Jelena Filipović Tričković, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 71 (2020): Issue 4 (December 2020)Next article