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Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii Cover

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 3 (September 2020)
By: ,  ,   and    
Open Access
|Oct 2020
Figures & tables

Figures & Tables

Table 1

Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of juniper and immortelle essential oils in nontuberculous mycobacteria

Essential oilM. aviumMIC / MBC (mg/mL) M. intracellulareM. gordonae
Common juniper1.6 / 1.61.6 / 1.61.6 / 1.6
Immortelle3.2 / 3.23.2 / 3.23.2 / 3.2
Table 2

The lowest concentrations of juniper and immortelle essential oils that were the most effective against NTMs in the checkerboard synergy method

Essential oilM. aviumM. intracellulareM. gordonae
Immortelle0.0060.0121.60.0060.40.8
Juniper0.80.80.81.60.20.1
FICHI0.0010.0040.50.0020.130.25
FICJU0.50.50.51.00.130.06
FICi0.50.51.01.00.260.31
InteractionSySyAdAdSySy

[i] * concentration of essential oil in mg/mL; FICHI – fractional inhibitory concentration for immortelle EO; FICi – fractional inhibitory concentration index; FICJU – fractional inhibitory concentration for juniper EO; Sy – synergistic effect; Ad– additive effect

Table 3

Inhibitory (IC50) and lethal (LC50) concentrations (in mg/mL) of juniper and immortelle essential oils on A. castellanii cells

Essential oilIC50LC50
Juniper0.7200.800
Immortelle0.5500.620
Figure 1

Effects (means ± SD) of juniper and immortelle EOs at three-quarters of the MIC on the adhesion of M. avium, M. intracellulare and M. gordonae to A. castellanii expressed as CFU/mL and the percentage (%) of adhesion inhibition. CFU – colony forming units; HI – Helichrysum italicum (immortelle); JU – Juniperus communis (juniper); MIC – minimum inhibitory concentration. Statistically significant differences between groups: * (p=0.001); ** (p=0.0002); *** (p=0.0021); **** (p=0.0034); ***** (p=0.00008); ****** (p=0.00008)

Figure 2

Effects (means ± SD) of subinhibitory concentrations of juniper and immortelle EOs (in mg/mL) on the adhesion of M. avium (A), M. intracellulare (B) and M. gordonae (C) on A. castellanii expressed as CFU/mL and the percentage (%) of adhesion inhibition. CFU – colony forming units; HI – Helichrysum italicum (immortelle); JU – Juniperus communis (juniper). Statistically significant differences between groups: * (p=0.0246); ** (p=0.0266); *** (p=0.0050); **** (p=0.00032)

Figure 3

Adhesion of M. avium, M. intracellulare, and M. gordonae on A. castellanii under a light microscope at 1000x magnification (Ziehl-Neelsen staining). a) control without essential oil; b) after treatment with juniper essential oil at a subinhibitory concentration

DOI: https://doi.org/10.2478/aiht-2020-71-3442 | Journal eISSN: 1848-6312 (formerly 0004-1254) | Journal ISSN: 0004-1254
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Language: English, Croatian
Page range: 223 - 230
Submitted on: May 1, 2020
Accepted on: Sep 1, 2020
Published on: Oct 6, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Keywords:
Acanthamoeba castellanii,
antiadhesion,
Helichrysum italicum,
Juniperus communis,
Mycobacterium avium,
Mycobacterium gordonae,
Mycobacterium intracellulare
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2020 Dolores Peruč, Brigita Tićac, Dalibor Broznić, Ivana Gobin, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 71 (2020): Issue 3 (September 2020)
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