Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii Cover

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Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Archives of Industrial Hygiene and Toxicology

Volume 71 (2020): Issue 3 (September 2020)

By: Dolores Peruč, Brigita Tićac, Dalibor Broznić and Ivana Gobin

Open Access

|Oct 2020

Figures & Tables

Effects (means ± SD) of juniper and immortelle EOs at three-quarters of the MIC on the adhesion of M. avium, M. intracellulare and M. gordonae to A. castellanii expressed as CFU/mL and the percentage (%) of adhesion inhibition. CFU – colony forming units; HI – Helichrysum italicum (immortelle); JU – Juniperus communis (juniper); MIC – minimum inhibitory concentration. Statistically significant differences between groups: * (p=0.001); ** (p=0.0002); *** (p=0.0021); **** (p=0.0034); ***** (p=0.00008); ****** (p=0.00008)

Effects (means ± SD) of subinhibitory concentrations of juniper and immortelle EOs (in mg/mL) on the adhesion of M. avium (A), M. intracellulare (B) and M. gordonae (C) on A. castellanii expressed as CFU/mL and the percentage (%) of adhesion inhibition. CFU – colony forming units; HI – Helichrysum italicum (immortelle); JU – Juniperus communis (juniper). Statistically significant differences between groups: * (p=0.0246); ** (p=0.0266); *** (p=0.0050); **** (p=0.00032)

Adhesion of M. avium, M. intracellulare, and M. gordonae on A. castellanii under a light microscope at 1000x magnification (Ziehl-Neelsen staining). a) control without essential oil; b) after treatment with juniper essential oil at a subinhibitory concentration

Inhibitory (IC50) and lethal (LC50) concentrations (in mg/mL) of juniper and immortelle essential oils on A_ castellanii cells

Essential oil	IC₅₀	LC₅₀
Juniper	0.720	0.800
Immortelle	0.550	0.620

Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of juniper and immortelle essential oils in nontuberculous mycobacteria

Essential oil	M. avium	MIC / MBC (mg/mL) M. intracellulare	M. gordonae
Common juniper	1.6 / 1.6	1.6 / 1.6	1.6 / 1.6
Immortelle	3.2 / 3.2	3.2 / 3.2	3.2 / 3.2

The lowest concentrations of juniper and immortelle essential oils that were the most effective against NTMs in the checkerboard synergy method

Essential oil	M. avium		M. intracellulare		M. gordonae
Immortelle	0.006	0.012	1.6	0.006	0.4	0.8
Juniper	0.8	0.8	0.8	1.6	0.2	0.1
FIC_HI	0.001	0.004	0.5	0.002	0.13	0.25
FIC_JU	0.5	0.5	0.5	1.0	0.13	0.06
FIC_i	0.5	0.5	1.0	1.0	0.26	0.31
Interaction	Sy	Sy	Ad	Ad	Sy	Sy

DOI: https://doi.org/10.2478/aiht-2020-71-3442 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254

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Language: English, Croatian, Slovenian

Page range: 223 - 230

Submitted on: May 1, 2020

Accepted on: Sep 1, 2020

Published on: Oct 6, 2020

Published by: Institute for Medical Research and Occupational Health

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Related subjects:

Basic medical science,

Basic medical science, other

© 2020 Dolores Peruč, Brigita Tićac, Dalibor Broznić, Ivana Gobin, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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