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Nephrotoxicity and genotoxicity of silver nanoparticles in juvenile rats and possible mechanisms of action

Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 2 (June 2020)
By:
Ye Liu,  Li Sun,  Guili Yang and  Zhuo Yang  
Open Access
|Jun 2020

Figures & Tables

Figure 1

Transition electron microscopy of silver nanoparticles in suspension
Transition electron microscopy of silver nanoparticles in suspension

Figure 2

Experimental design of AgNP administration, sampling, and tissue collection; AgNP – silver nanoparticles; H&E – haematoxylin and eosin; ELISA – enzyme-linked immunosorbent assay
Experimental design of AgNP administration, sampling, and tissue collection; AgNP – silver nanoparticles; H&E – haematoxylin and eosin; ELISA – enzyme-linked immunosorbent assay

Figure 3

Mean (±SEM) body weight (A), urinary microalbumin (B), serum creatinine (C), tail intensity (comet assay) (D), the frequency of micronucleated PCEs in bone marrow (E), and the PCE to NCE ratio (F) in control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; AgNP – silver nanoparticles; NCE – normochromatic erythrocytes; PCE – polychromatic erythrocytes; SEM – standard error of the mean
Mean (±SEM) body weight (A), urinary microalbumin (B), serum creatinine (C), tail intensity (comet assay) (D), the frequency of micronucleated PCEs in bone marrow (E), and the PCE to NCE ratio (F) in control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; AgNP – silver nanoparticles; NCE – normochromatic erythrocytes; PCE – polychromatic erythrocytes; SEM – standard error of the mean

Figure 4

Mean (±SEM) ROS GSH, SOD IL-6, IL-1β, and TNF-α in kidney cells and tissue of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; GSH – glutathione; IL – interleukin; ROS – reactive oxygen species; SEM – standard error of the mean; SOD – superoxide dismutase; TNF – tumour necrosis factor
Mean (±SEM) ROS GSH, SOD IL-6, IL-1β, and TNF-α in kidney cells and tissue of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; GSH – glutathione; IL – interleukin; ROS – reactive oxygen species; SEM – standard error of the mean; SOD – superoxide dismutase; TNF – tumour necrosis factor

Figure 5

Mean (±SEM) NOX4 and TRPC6 in rat renal cortex (normalised to β-actin expression) of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; NOX – NADPH oxidase 4; SEM – standard error of the mean; TRPC6 – transient receptor potential cation channel subfamily C member 6
Mean (±SEM) NOX4 and TRPC6 in rat renal cortex (normalised to β-actin expression) of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; NOX – NADPH oxidase 4; SEM – standard error of the mean; TRPC6 – transient receptor potential cation channel subfamily C member 6

Figure 6

TRPC6 immunofluorescence staining of the renal cortex (50 μm scale bar) (A) and mean (±SEM) fluorescent intensity (B) in the glomeruli of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; SEM – standard error of the mean; TRPC6 – transient receptor potential cation channel subfamily C member 6
TRPC6 immunofluorescence staining of the renal cortex (50 μm scale bar) (A) and mean (±SEM) fluorescent intensity (B) in the glomeruli of control and juvenile rats treated with AgNPs for one, two, or three weeks (n=8 per group); *P<0.05; SEM – standard error of the mean; TRPC6 – transient receptor potential cation channel subfamily C member 6

Figure 7

Histological findings in the kidney of juvenile rats: (A) control group, (B) 1-wk-AgNP group (C) 2-wk- AgNP group; (D) 3-wk-AgNP group (100 μm scale bar; arrows indicate typically affected areas)
Histological findings in the kidney of juvenile rats: (A) control group, (B) 1-wk-AgNP group (C) 2-wk- AgNP group; (D) 3-wk-AgNP group (100 μm scale bar; arrows indicate typically affected areas)

Figure 8

Diagram of the main likely mechanisms of AgNP nephrotoxicity based on oxidative stress; AgNP – silver nanoparticles; H&E – haematoxylin and eosin; ELISA – enzyme-linked immunosorbent assay; GSH – glutathione; ROS – reactive oxygen species; SOD – superoxide dismutase; TRPC6– transient receptor potential cation channel subfamily C member 6
Diagram of the main likely mechanisms of AgNP nephrotoxicity based on oxidative stress; AgNP – silver nanoparticles; H&E – haematoxylin and eosin; ELISA – enzyme-linked immunosorbent assay; GSH – glutathione; ROS – reactive oxygen species; SOD – superoxide dismutase; TRPC6– transient receptor potential cation channel subfamily C member 6
DOI: https://doi.org/10.2478/aiht-2020-71-3364 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 121 - 129
Submitted on: Nov 1, 2019
Accepted on: May 1, 2020
Published on: Jun 29, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
alkaline comet assay,
inflammation,
oxidative stress,
trpc6 cation channel
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2020 Ye Liu, Li Sun, Guili Yang, Zhuo Yang, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

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