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Reproductive disorders in male rats induced by high-fructose consumption from juvenile age to puberty Cover

Reproductive disorders in male rats induced by high-fructose consumption from juvenile age to puberty

Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 1 (March 2020)
By: Oleksandr Y. Tkachenko,  Ganna M. Shayakhmetova,  Anatoliy V. Matvienko and  Valentina M. Kovalenko  
Open Access
|Apr 2020

Figures & Tables

Figure 1

Photomicrographs of rat testes stained with haematoxylin and eosin; (A) normal structure of seminiferous tubules in control rats (200x magnification); (B) focal dystrophic changes in spermatogonia (spg) and primary spermatocytes (spc) in HF rats (400x magnification); (C) spermatogenic epithelium exfoliation into the lumens of seminiferous tubules in HF rats (200x magnification); (D) decrease in the number of spermatogenic epithelium layers and expansion of the seminiferous tubules in HF rats (400x magnification)
Photomicrographs of rat testes stained with haematoxylin and eosin; (A) normal structure of seminiferous tubules in control rats (200x magnification); (B) focal dystrophic changes in spermatogonia (spg) and primary spermatocytes (spc) in HF rats (400x magnification); (C) spermatogenic epithelium exfoliation into the lumens of seminiferous tubules in HF rats (200x magnification); (D) decrease in the number of spermatogenic epithelium layers and expansion of the seminiferous tubules in HF rats (400x magnification)

Figure 2

Photomicrographs of rats epididymis stained with haematoxylin and eosin; (A and B) normal epididymis tubule structure in control rats (100x and 400x magnification, respectively); (C) focal luminal sperm stasis in HF rats (100x magnification); (D) epithelial clear cell hyperplasia in HF rats (400x magnification)
Photomicrographs of rats epididymis stained with haematoxylin and eosin; (A and B) normal epididymis tubule structure in control rats (100x and 400x magnification, respectively); (C) focal luminal sperm stasis in HF rats (100x magnification); (D) epithelial clear cell hyperplasia in HF rats (400x magnification)

Spermatogenesis epithelium findings

ParametersGroups
P value
ControlHigh-fructose
Spermatogenesis index (stages of spermatogenesis total / number of examined tubules)3.44±0.21 3.43 (3.42–3.45)3.34±0.05 3.34 (3.31–3.38)<0.001*
Number of spermatogonia (per tubular cross section)71.83±5.43 71.64 (68.38–75.28)59.80±4.24 59.64 (56.99–62.61)<0.001*
Cells at the 12th stage of spermatogenesis (%)4.00 (3.0; 4.5)2.53 2.83(2.03±1.21 –3.64)0.008*
Exfoliation of epithelium (%)1.0 (1.0; 2.0)2.5 (1.5; 3.0)1.000

Comparison of gonad weight, volume, and sperm counts between the high-fructose-treated and control group

Groups
ParametersControlHigh-fructoseP value
Testes absolute weight (g)3.48 3.49(3.31±0.24 –3.68)3.20 3.21(3.06±0.19 –3.36)0.014*
Testes relative weight (% of body weight)1.16 1.17(1.10±0.08–1.23) )1.27 1.28(1.15±0.17 –1.41)0.09
Testes volume (cm3)3.79 3.81(3.53±0.39 –4.10)3.34 3.37(3.05±0.45 –3.69)0.029*
Epididymis absolute weight (g)0.96 (0.95; 0.98)0.88 0.88(0.81±0.11 –0.95)0.047*
Epididymis relative weight (% of body weight)0.32 0.33(0.30±0.04 –0.35)0.35 0.35(0.32±0.02 –0.38)0.217
Sperm count (million/mL)68.43 70.26(53.23±6.96 –87.28)52.01 52.57(46.31±3.19 –58.82)0.046*

Comparison of serum sex hormone levels between the high-fructose-treated and control group

ParametersGroups
P value
ControlHigh-fructose
T (nmol/L)14.69 14.96(12.09±3.10 –17.82)9.34 (10.25; 12.25)0.024*
LH (ng/mL)0.301±0.231) 0.174 (0.124–0.478)0.691±0.189 0.622 (0.505–0.797)0.003*
FSH (ng/mL)0.077 0.10(0.044±0.06 –0.157)0.218 (0.171; 0.235)0.021*

Fertilising capacity of male rats

GroupsNumber of mated femalesNumber of pregnant femalesFertilising capacity
Control423891 %
High-fructose group424198 %
P value--0.809

Fertility rate of unexposed females mated with HF males

GroupsParameters
Total number of foetusesNumber of foetuses per female
Control3199.5 (7.5; 10.5)
High-fructose group3009 (6; 9)
P value-0.092

Effects of paternal high-fructose treatment on embryogenesis/foetogenesis in unexposed females on day 20 of gestation

Groups
ParametersControlHigh-fructoseP value
Number of pregnant females (N)3841-
Total number of corpora lutea (N)362394-
Number of corpora lutea per female (N)10.5 (8.5; 11)10 (9; 11)1.000
Preimplantational loss (N /%)25 / 1060 / 150.012*
Preimplantational loss per female (N)0 (0; 1)1 (0; 2)0.028*
Postimplantational loss (N /%)6 / 219 / 60.013*
Postimplantational loss per female (N)0 (0; 0)0 (0; 1)0.041*
Total embryonal/foetal death (%)11.8823.86<0.001*
DOI: https://doi.org/10.2478/aiht-2020-71-3303 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
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Language: English, Croatian, Slovenian
Page range: 78 - 86
Submitted on: Jun 1, 2019
Accepted on: Feb 1, 2020
Published on: Apr 9, 2020
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
epididymis,
fertility,
FSH,
LH,
male reproductive function,
metabolic syndrome,
MetS,
sperm count,
testes,
testosterone
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2020 Oleksandr Y. Tkachenko, Ganna M. Shayakhmetova, Anatoliy V. Matvienko, Valentina M. Kovalenko, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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