Analysis of Inter-Primer Binding Site Retrotransposon Length Polymorphism in Selected Group of Vaccinium corymbosum

Klongová, Lucia; Bilčíková, Jana; Urbanová, Lucia; Žiarovská, Jana

doi:10.2478/ahr-2024-0017

Abstract

The genetic variability in 13 selected genotypes of Vaccinium corymbosum using re-trotransposon-based iPBS markers system was characterized in this study. The four selected iPBS primers amplified 232 fragments, average 58 fragments per primer, approximately 53.16% of which were polymorphic. The polymorphism information content of iPBS marker was 0.238. The hierarchical cluster using unweighted pair group method with arithmetic mean analysis divided selected genotypes into two main groups and several subgroups. The values of the Jaccard coefficient of genetic relatedness were between 0.036–0.243. The similarity between two sample sets was 3.6%–24.3%. This study confirmed inter-PBS amplification technique as a reproducible and trustable marker system, but has proved to be an effective DNA fingerprinting technology to generate polymorphism in Vaccinium corymbosum.

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Analysis of Inter-Primer Binding Site Retrotransposon Length Polymorphism in Selected Group of Vaccinium corymbosum

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