Total sample description and detection of DNA-HPV in relation with gender and age groups
| Patients | Positive HPV result | |
|---|---|---|
| Male | 82 | 11 |
| Female | 57 | 3 |
| 19–39 years old | 106 | 12 |
| 40–59 years old | 9 | 0 |
| 60+ years old | 24 | 2 |
Conditioning protocol of nested PCR technique using primer pairs: MY09/11 and GP5+/6+
| Set primers | Reaction step | Temperature, °C | Duration | Number of repetitions |
|---|---|---|---|---|
| MY09/11 | Initial denaturation | 95 | 3 min | - |
| Denaturation | 95 | 30 s | 40× | |
| Hybridization of primers | 53 | 30 s | ||
| Amplification DNA | 72 | 45 s | ||
| Final amplification | 72 | 5 min | - | |
| - | 4 | ∞ | - | |
| GP5+/GP6+ | Initial denaturation | 94 | 5 min | - |
| Denaturation | 94 | 1 min | 30× | |
| Hybridization of primers | 48 | 2 min | ||
| Amplification DNA | 72 | 1 min | ||
| Final amplification | 72 | 5 min | - | |
| - | 4 | ∞ | - |