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Expression and biochemical significance of Piwil2 in stem cell lines Cover

Expression and biochemical significance of Piwil2 in stem cell lines

Postępy Higieny i Medycyny Doświadczalnej
Volume 76 (2022): Issue 1 (January 2022)
By: Dilek Kaan  
Open Access
|Apr 2022

Figures & Tables

Fig. 1

A. Morphologies of MCF10A cells with inverted microscope. B. MCF-7cells (x200 magnification)
A. Morphologies of MCF10A cells with inverted microscope. B. MCF-7cells (x200 magnification)

Fig. 2

A. Comparison of GST A1 activities of non-transfected MCF10A cells and MCF10A/Piwil2 cells by using CDNB as substrate. B. Comparison of GST A1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2 by using CDNB as substrate.
A. Comparison of GST A1 activities of non-transfected MCF10A cells and MCF10A/Piwil2 cells by using CDNB as substrate. B. Comparison of GST A1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2 by using CDNB as substrate.

Fig. 3

A. Comparison of GST P1 activities of non-transfected MCF10A cells and MCF10A/Piwil2, B. Comparison of GST P1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2. Error bars represent standard deviation from the mean (┬)
A. Comparison of GST P1 activities of non-transfected MCF10A cells and MCF10A/Piwil2, B. Comparison of GST P1 activities of non-transfected MCF-7 cells and MCF-7/Piwil2. Error bars represent standard deviation from the mean (┬)

Fig. 4

Molecular characterization with fluorescence microscopy of cell lines. A. Non-transfected MCF10A. B. Non-transfected MCF-7. (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).
Molecular characterization with fluorescence microscopy of cell lines. A. Non-transfected MCF10A. B. Non-transfected MCF-7. (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).

Fig. 5

A. Immunocytochemistry of Piwil2 transfected cell line MCF10A with an antibody against Piwil2 protein. B. Immunocytochemistry of Piwil2 transfected cell line MCF-7 with an antibody against Piwil2 protein (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).
A. Immunocytochemistry of Piwil2 transfected cell line MCF10A with an antibody against Piwil2 protein. B. Immunocytochemistry of Piwil2 transfected cell line MCF-7 with an antibody against Piwil2 protein (Cells expressing Piwil2 are indicated by arrows) (FITC, green signal) (x200 magnification).
DOI: https://doi.org/10.2478/ahem-2022-0009 | Journal eISSN: 1732-2693
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Language: English
Page range: 97 - 103
Submitted on: Apr 20, 2021
Accepted on: Sep 23, 2021
Published on: Apr 2, 2022
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
cancer stem cell,
ethacrynic acid,
glutathione -transferase,
piwil2
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2022 Dilek Kaan, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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