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Costal cartilage ensures low degradation of DNA needed for genetic identification of human remains retrieved at different decomposition stages and different postmortem intervals

Postępy Higieny i Medycyny Doświadczalnej
Volume 75 (2021): Issue 1 (January 2021)
By:
Open Access
|Dec 2021

Figures & Tables

Figure 1

Anatomical location of costal cartilage in the rib cage (light gray color). The arrow shows the place from which the research material was collected
Anatomical location of costal cartilage in the rib cage (light gray color). The arrow shows the place from which the research material was collected

Figure 2

Morphological changes in costal cartilage at different times elapsed since death and at different exposure times to environmental factors: A – few days after death; B – few weeks after death; C – 7 months after death; D – 2 years and 11 months after death
Morphological changes in costal cartilage at different times elapsed since death and at different exposure times to environmental factors: A – few days after death; B – few weeks after death; C – 7 months after death; D – 2 years and 11 months after death

Figure 3

Morphological changes in costal cartilage in almost skeletonized cadavers: A – remains of costal cartilage collected from 67-year-old male cadaver of unknown identity, found in the converted and abandoned bus 14 months after death; B – remains of costal cartilage collected from male cadaver of unknown identity and age, found in a ditch 3 years after death
Morphological changes in costal cartilage in almost skeletonized cadavers: A – remains of costal cartilage collected from 67-year-old male cadaver of unknown identity, found in the converted and abandoned bus 14 months after death; B – remains of costal cartilage collected from male cadaver of unknown identity and age, found in a ditch 3 years after death

Figure 4

Places in which the cadavers included in the study were found
Places in which the cadavers included in the study were found

Figure 5

DNA concentration [ng/ μl] after T. Large autosomal sequence (214 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments
DNA concentration [ng/ μl] after T. Large autosomal sequence (214 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments

Figure 6

DNA concentration [ng/ μl] after T. Small autosomal sequence (80 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments
DNA concentration [ng/ μl] after T. Small autosomal sequence (80 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments

Figure 7

DNA concentration [ng/ μl] after the Y chromosome sequence (75 bp) amplification in costal cartilage samples collected from male cadavers retrieved from different environments
DNA concentration [ng/ μl] after the Y chromosome sequence (75 bp) amplification in costal cartilage samples collected from male cadavers retrieved from different environments

Figure 8

DNA degradation index* in costal cartilage samples collected from cadavers retrieved from different environments. *T. Large amplicon DNA concentration/T. Small amplicon DNA concentration
DNA degradation index* in costal cartilage samples collected from cadavers retrieved from different environments. *T. Large amplicon DNA concentration/T. Small amplicon DNA concentration

Statistical analysis of DNA concentration measured for different loci in costal cartilage samples collected from cadavers retrieved from different environments_ The results are presented as median (lower – upper quartile)_ Statistical significance was set at p > 0_05

Cadaver retrieval location
Variable1 Indoors (flat/after hospitalization) n = 192 Outdoors (primarily in forest area) n = 243 Water/exhumation site n = 54 Coal mine n = 95 Fire site n = 66 Basement/uninhabited building n = 77 Unknown location n = 9p
T. Large amplicon DNA concentration [ng/μl] n = 794.8 (2.6-8.7)4.8 (2.5-9.7)8.4 (3.9-14.1)9.1 (7.6-11.6)4.6 (2.3-13.7)6.5 (4.8-7.3)6.2 (4.4-19.5)0.143
T. Small amplicon DNA concentration [ng/μl] n = 793.8 (2.3-8.2)3.8 (2.3-7.1)7.1 (2.5-13.4)7.1(6.2-9.2)4.9 (2.5-10.0)6.6 (4.3-7.8)4.8 (3.7-16.7)0.146
Y amplicon DNA concentration [ng/μl] n = 686.0 (3.9-9.8) n = 164.3 (2.3-7.1) n = 229.3 (3.2-17.2) n = 48.7 (7.2-13.3) n = 99.5 (5.4-14.9) n = 46.4 (8.8-7.9) n = 610.2 (4.4-21.3) n = 70.132
Degradation index* n = 790.9 (0.8-1.0)0.8 (0.7-0.9)0.9 (0.6-0.9)0.9 (0.8-0.9)1.0 (0.8-1.1)1.0 (0.7-1.1)0.8 (0.8-0.9)0.503
DOI: https://doi.org/10.2478/ahem-2021-0035 | Journal eISSN: 1732-2693
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Language: English
Page range: 852 - 858
Submitted on: Dec 7, 2020
Accepted on: Jun 24, 2021
Published on: Dec 4, 2021
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
costal cartilage,
advanced decomposition,
STR,
DNA degradation index,
postmortem interval
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2021 Marcin Tomsia, Kornelia Droździok, Gulnaz T. Javan, Rafał Skowronek, Michał Szczepański, Elżbieta Chełmecka, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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