Figure 1
The demographical characteristics of the patient and control groups
| Variables | Patient Group (n: 129) | Control Group (n: 146) | p | |
|---|---|---|---|---|
| Age (mean ± SD) | 61.24 ± 8.345 | 63.55 ± 7.92 | >0.05 | |
| F | 35 (27.1%) | 36 (24.7%) | >0.05 | |
| Gender | ||||
| M | 94 (72.9%) | 110 (75.3%) | >0.05 | |
| Yes | 50 (38.8%) | 53 (36.3%) | >0.05 | |
| Smoking | ||||
| No | 79 (61.2%) | 93 (63.7%) | >0.05 | |
| Total Cholesterol (mg/dL) | 194.63 ± 53.71 | 220.54 ± 47 | 0.0001 | |
| Triglyceride (mg/dL) | 142.38 ± 106.59 | 148.29 ± 110.03 | 0.651 | |
| High-density lipoprotein (mg/dL) | 51.06 ± 17.44 | 47.95 ± 18.16 | 0.149 | |
| Low-density lipoprotein (mg/dL) | 112.14 ± 40.24 | 130.23 ± 42.05 | 0.0001 | |
| Fasting blood sugar (mg/dL) | 107.49 ± 23.49 | 107.74 ± 27.09 | 0.936 | |
| H. cinaedi | 6 (4.65%) | 0 (0%) | 0.010 | |
Results of logistic regressions according to the variables in the patient group
| B | S.E. | Wald | df | Sig. | OR | 95% C.I. | ||
|---|---|---|---|---|---|---|---|---|
| Lower | Upper | |||||||
| Age | 0.041 | 0.017 | 5.753 | 1 | 0.016 | 1.042 | 1.008 | 1.078 |
| Gender (male) | 0.001 | 0.153 | 0.000 | 1 | 0.997 | 1.001 | 0.742 | 1.349 |
| Total (≥200 Cholesterol mg/dL) | 0.615 | 0.151 | 16.470 | 1 | 0.000 | 1.849 | 1.374 | 2.488 |
| (≥Triglyceride 150 mg/dL) | 0.071 | 0.147 | 0.230 | 1 | 0.632 | 1.073 | 0.804 | 1.432 |
| High-density (≥50 mg/lipoprotein dL) | -0.295 | 0.143 | 4.273 | 1 | 0.039 | 0.745 | 0.563 | 0.985 |
| Low-density (≥100 mg/lipoprotein dL) | 0.193 | 0.165 | 1.371 | 1 | 0.242 | 1.213 | 0.878 | 1.676 |
| Fasting (≥125 Blood mg/dL) Sugar | -0.160 | 0.176 | 0.831 | 1 | 0.362 | 0.852 | 0.604 | 1.202 |
| H. cinaedi presence | 10.769 | 7720.80 | 0.000 | 1 | 0.999 | 47527 | 0.000 | |
| Constant | 7.824 | 7720.80 | 0.000 | 1 | 0.999 | 2499.6 | ||
Comparison of patient and control groups for various variables
| Variables | Patient Group | Control Group | χ2 | p | OR | %95 CI | |
|---|---|---|---|---|---|---|---|
| Min | Max | ||||||
| Smoking | 50 | 53 | 0.177 | 0.674 | 1.11 | 0.681 | 1.811 |
| Total* (≥200 Cholesterol mg/dL) | 96 | 64 | 26.32 | 0.001 | 3.727 | 2.231 | 6.226 |
| Triglyceride* (≥150 mg/dL) | 41 | 22 | 0.296 | 0.587 | 1.154 | 0.689 | 1.932 |
| High-density* (≥50 mg/lipoprotein dL) | 80 | 103 | 2.240 | 0.135 | 0.682 | 0.412 | 1.127 |
| Low-density* (≥100 mg/lipoprotein dL) | 102 | 92 | 8.497 | 0.004 | 2.217 | 1.291 | 3.809 |
| Fasting (≥125 blood* mg/dL) sugar | 20 | 29 | 0.889 | 0.346 | 0.740 | 0.396 | 1.385 |
| H. cinaedi presence** | 6 | 0 | - | 0.010 | - | 0.401 | 0.521 |
Primers used in the nested-PCR method [5]
| Sequences (5’-3’) | Product size | |
|---|---|---|
| H.cinaedi | 1F: GGA GCT GTG AGT GTG CTG 1R: AAA TGA CCG ACA CGA GCT G | 659 bp |
| H. cinaedi (nested PCR) | 2F: GGA TTT AGG CTC TCG CTC TCG TCC GGA TAT 2R: ATC GCC GTC CTT ACT CGC GTC TCC AA ATTA | 359 bp |
| Beta-globin | F: ACA CAA CTG TGT TCA CTA GC R: GGA AAA TAG ACC AAT AGG CTG | 251 bp |