Effect of Bacterial Biofilms on Topical Antimicrobial Therapy
References
- Chegini, Z., Azizi, M., Safaiee, M., Kalhori, F., Zare Shahraki, R., Hemmati, J., Hosseini, S. M., Teimoori, A., Shariati, A., & Arabestani, M. R. (2025). Wound healing properties and antibiofilm activity of hydrogel matrix containing nitric oxide, silver nanoparticles, and ciprofloxacin against Pseudomonas aeruginosa burn wound infection. Scientific Reports, 15, 26508.
https://doi.org/10.1038/s41598-025-12385-w - Fonseca, A. P. (2011). Biofilm in wounds: An unsolved problem? EWMA Journal, 11, 10–24.
- Hammond, A. A., Miller, K. G., Kruczek, C. J., Dertien, J., Colmer-Hamood, J. A., Griswold, J. A., Horswill, A. R., & Hamood, A. N. (2011). An in vitro biofilm model to examine the effect of antibiotic ointments on biofilms produced by burn wound bacterial isolates. Burns, 37(2), 312–321.
https://doi.org/10.1016/j.burns.2010.09.001 - Kessler, J., & Hooper, S. E. (2025). Topical molecular iodine: An optimal biocide constrained by inadequate formulations. International Journal of Molecular Sciences, 26, 4853.
https://doi.org/10.3390/ijms26094853 - Khan, M., King, E., Breisinger, K., Serena, L., & Serena, T. E. (2024). A double-blind trial comparing an antimicrobial combination to standard care in hard-to-heal wounds. Journal of Wound Care, 33(2), 84–86.
- Kirketerp-Møller, K., Bjarnsholt, T., & Thomsen, T. R. (2011). The fight against biofilm infection: Do we have knowledge and means? EWMA Journal, 11, 7–8.
- Lethongkam, S., et al. (2025). Hydrogel dressing containing natural extracts inhibits dual-species biofilms of Staphylococcus aureus and Acinetobacter baumannii in ex vivo wound infection models. Scientific Reports, 15, 34599.
https://doi.org/10.1038/s41598-025-34599 - Mezzasalma, N., Spadini, C., Spaggiari, C., Annunziato, G., Andreoli, V., Prosperi, A., Mochen, L., Cavirani, S., Grolli, S., Taddei, S., Costantino, G., & Cabassi, C. S. (2025). Antibacterial and antibiofilm activity of Eucalyptus globulus leaf extract, asiatic acid and ursolic acid against bacteria isolated from bovine mastitis. Frontiers in Veterinary Science, 12, 1565787.
https://doi.org/10.3389/fvets.2025.1565787 - Motelica, L., Ficai, D., Petrisor, G., Oprea, O.-C., Truşcă, R.-D., Ficai, A., Andronescu, E., Hudita, A., & Holban, A. M. (2024). Antimicrobial hydroxyethyl-cellulose-based composite films with zinc oxide and mesoporous silica loaded with cinnamon essential oil. Pharmaceutics, 16, 1225.
https://doi.org/10.3390/pharmaceutics16091225 - Muslim, M. R. F., Chabib, L., Suryaningsih, B. E., Hayati, F., & Annisa, V. (2025). Green-synthesized silver nanoparticle hydrogels for biofilm-infected wounds: Bridging sustainability and clinical translation. Frontiers in Pharmacology, 16, 1694144.
https://doi.org/10.3389/fphar.2025.1694144 - Roche ED, Renick PJ, Tetens SP, Carson DL. A model for evaluating topical antimicrobial efficacy against methicillin-resistant Staphylococcus aureus biofilmsin superficial murine wounds. Antimicrob Agents Chemother. 2012; 56(8): 4508–10
- Schneider, R. E., Hamdan, J. V., & Rumbaugh, K. P. (2025). Biofilm dispersal and wound infection clearance with preclinical debridement agents. International Wound Journal, 22, e70145.
https://doi.org/10.1111/iwj.70145 - Stepanović, S., Vuković, D., Hola, V., Di Bonaventura, G., Djukic, S., Cirkovic, I., & Ruzicka, F. (2007). Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS, 115(8), 891–899.
https://doi.org/10.1111/j.1600-0463.2007.apm_630.x - Thomas, Z. M., et al. (2025). Antimicrobial efficacy and stability of an N-chlorotaurine gel for chronic wound treatment. International Journal of Molecular Sciences, 26, 8677.
https://doi.org/10.3390/ijms26178677 - Watson, F., Chen, R., Saint Bezard, J., & Percival, S. (2024). Comparison of antimicrobial efficacy and therapeutic index properties for common wound cleansing solutions, focusing on solutions containing PHMB. GMS Hygiene and Infection Control, 19, Doc73.
https://doi.org/10.3205/dgkh000528
DOI: https://doi.org/10.2478/afpuc-2026-0004 | Journal eISSN: 2453-6725
Language: English
Submitted on: Apr 20, 2026
Accepted on: May 15, 2026
Published on: Jun 24, 2026
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
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© 2026 Marianna Hajská, Peter Záhorec, Arpád Panyko, Martin Dubovský, Škanderová Martina, Marián Vidiščák, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.