DNA Cleavage and Cytotoxic Activity of Copper(II) Complexes Based on Reduced Schiff Bases Derived From Salicylaldehyde and Amino Acids

Korcová, Jana; Húserková, Mária; Lintnerová, Lucia; Valentová, Jindra

doi:10.2478/afpuc-2024-0003

Abstract

Metal complexes, which, under physiologic conditions, show redox properties and are able to bind to DNA, are great tools to cleave the DNA chain. This aspect is of great importance for their use as antineoplastic drugs. We synthesized ligands derived from short-chain amino acids and from salicylaldehyde. The prepared ligands of the type of reduced Schiff bases were subsequently used for the preparation of copper(II) complexes. The aim of the study was in vitro testing of copper(II) complexes, where it was confirmed that they are capable of cleaving DNA. Their cytotoxic activity was also confirmed by the resazurin redox method on Saccharomyces cerevisiae based on preserved healthy mitochondrial function.

References

Santini, C., Pellei, M., Gandin, V., Porchia, M., Tisato, F., Marzano, C. (2014) Advances in copper complexes as anticancer agents. Chem Rev 114:815–862.
Search in Google Scholar Back to article
Li, D.D., Zhang, N., Dai, L.L., Yang, Z.B, Tao, Z.W. (2016). Synthesis, DNA binding, nuclease activity and cytotoxic studies of a wheel-shaped octanuclear copper(II) complex based on 1,2,4-triazole. Appl Organometal Chem 30:346–353.
Search in Google Scholar Back to article
Qi, J.X., Liang, S.C., Gou, Y.,, Zhang Z.L., Zhou, Z.P., Yang, F., Liang, H. (2015) Synthesis of four binuclear copper(II) complexes: structure, anticancer properties and anticancer mechanism. Eur J Med Chem 96:360–368.
Search in Google Scholar Back to article
Denoyer D, Masaldan S, La Fontaine S, Cater M.A (2015). Targeting copper in cancer therapy: ‘copper that cancer’. Metallomics 7:1459–1476.
Search in Google Scholar Back to article
Lintnerová L, Valentová J, Herich P, Kožíšek J, Devínsky F (2018). Synthesis and antiradical activity of novel copper(II) complexes of long chain reduced Schiff base ligands. Monatsh Chem – Chem Mon 149:901–911.
Search in Google Scholar Back to article
Cole, K. D., Tellez, C. M. (2002). Separation of large circular DNA by electrophoresis in agarose gels. Biotechnology progress 18(1):82–87.
Search in Google Scholar Back to article
Kumar, A., et al.(2012). Cu (II) complexes of glyco-iminoaromatic conjugates in DNA binding, plasmid cleavage and cell cytotoxicity. J Chem Sci 124(6):1217–1228.
Search in Google Scholar Back to article
Bitacura, J. G. (2018). The Use of Baker’s Yeast in the Resazurin Reduction Test: A Simple, Low-Cost Method for Determining Cell Viability in Proliferation and Cytotoxicity Assays. J Microbio. Biol Educ 19 (2):19–87. DOI: https://doi.org/10.1128/jmbe.v19i2.1599
Open DOI Search in Google Scholar Back to article
Botstein, D., Cherviz, S.A., Cherry, J.M. (1997). Yeast as a model organism. Science 277(5330):1259–1260.
Search in Google Scholar Back to article
Borra, R.C., Lotufo, M.A., Gagioti, S.M., Barros, F.D.M., Andrade, P.M. (2009). A simple method to measure cell viability in proliferation and cytotoxicity assays. Braz Oral Res 23(3):255–262.
Search in Google Scholar Back to article
Matuo, R., Sousa, F.G., Soares, D.G., Bonatto, D., Saffi, J., Escargueil, A.E., Larsen, A.K., Henriques, J.A. (2012). Saccharomyces cerevisiae as a model system to study the response to anticancer agents. Cancer Chemother Pharmacol 70:491–502.
Search in Google Scholar Back to article

DNA Cleavage and Cytotoxic Activity of Copper(II) Complexes Based on Reduced Schiff Bases Derived From Salicylaldehyde and Amino Acids

Abstract

Paradigm

My account