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Optimization of the Microscopic Method of Observing of the Oleogel Structure Cover

Optimization of the Microscopic Method of Observing of the Oleogel Structure

Open Access
|Aug 2023

Abstract

The subject of the research was the oleogel structure of the OraMAF oral suspension, containing a mixture of active substances in which the oleogel environment is formed by purified olive oil in combination with soy lecithin (SL) and sorbitan tristearate (STS). The objective of the research was the development of an optimal methodology for the work process, enabling microscopic observation of the structure of the oleogel suspension. Purified olive oil structured with a combination of gelators SL and STS with the addition of a solid phase of fucoidan (F) and chondroitin sulfate (CS) powders was used for the study as an alternative to OraMAF suspension. The sedimentation method of separation of the medium and staining of solid phases of the suspension brought the expected results, allowing the observation of the network structure of the gel, which consisted of interlaced gelator fibers assembling into star formations. The most interesting structures were clearly the star-like structures created from the star-shaped microtubules trapping the CS and F solid particles, colored blue.

Language: English
Page range: 58 - 61
Submitted on: Jun 22, 2023
Accepted on: Jun 23, 2023
Published on: Aug 24, 2023
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
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© 2023 J. Zima, E. Nováková, V. Mikušová, M. Šupolíková, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.