Anthocyanin-Rich Extract of Red Cabbage Attenuates Advanced Alcohol Hepatotoxicity in Rats in Association with Mitochondrial Activity Modulation

V. Buko; E. Belonovskaya; T. Kavalenia; T. Ilyich; S. Kirko; I. Kuzmitskaya; V. Moroz; E. Lapshina; A. Romanchuk; I. Zavodnik

doi:10.2478/afpuc-2022-0014

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Anthocyanin-Rich Extract of Red Cabbage Attenuates Advanced Alcohol Hepatotoxicity in Rats in Association with Mitochondrial Activity Modulation

European Pharmaceutical Journal

Volume 69 (2022): Issue 2 (August 2022)

By: V. Buko, E. Belonovskaya, T. Kavalenia, T. Ilyich, S. Kirko, I. Kuzmitskaya, V. Moroz, E. Lapshina, A. Romanchuk and I. Zavodnik

Open Access

|Aug 2022

Figures & Tables

Histological evaluation of the liver of rats with alcoholic steatohepatitis without or after the red cabbage extract (RCE) treatment. (a) Control; (b) Alcohol-treated group (ASH); (c) ASH + RCE (11 mg/kg), (d) ASH + RCE (22 mg/kg). Representative microphotographs of liver sections. The control group displayed normal liver architecture. The group administered with ethanol alone showed macro- and microvesicular liver steatosis, ballooning vacuolisation, scattered lymphocytic infiltration of portal tracts, and inflammatory foci. Macrovesicular fatty dystrophy and inflammatory foci were shown in the ASH group (black arrows). The signs of liver damage, degeneration, and inflammation were substantially reduced with the RCE treatment. The group treated with the larger dose of RCE was characterised mainly by microvesicular steatosis and scattered lymphoid infiltration. Rat liver sections were stained with Haematoxylin and Eosin (magnification: ocular 10×, objective 40×).

Effect of red cabbage extract (RCE) on the respiration parameters of isolated rat liver mitochondria. (a) The respiration rates V2 (1, 2) and V3 (3, 4); and (b) the coefficient of phosphorylation (ADP/O ratio) (1, 2) and the respiratory control ratio (RCR) (3, 4) (b) in the absence (1, 3) or in the presence of Ca2+ ions (60 μM) (2, 4). Mitochondria (0.5 mg protein/ml) were incubated in the medium containing 0.125 M KCl, 0.05 M sucrose, 0.01 M Tris-HCl, 0.0025 M KH2PO4, 0.005 M MgSO4, pH 7.4, at 25 °C, in the absence or in the presence of the anthocyanins (1.66–13.6 μg/ml). 5 mM succinate as substrate and 200 μM ADP were added. Significant difference (p < 0.05): * vs. that in the absence of RCE.

Representative traces of time-dependences of mitochondrial membrane potential dissipation. Control (1); 1.66 μg/ml anthocyanins (2); 3.34 μg/ml anthocyanins (3); 6.67 μg/ml anthocyanins (4); 13.6 μg/ml anthocyanins (5). The mitochondrial membrane potential was detected using the fluorescent dye safranin O (8 μM) at λex/λem 495/586 nm at 25 °C and 5 mM succinate as energising substrate. Mitochondria (0.3 mg of protein/ml) were added at constant gentle stirring to ethylene glycol tetraacetic acid (EGTA)-free medium: 0.05 M sucrose, 0.01 M Tris-HCl, 0.125 M КCl, 2.5 мM KH2PO4, 0.5 мM MgSO4, pH 7.4, 25° C, with or without red cabbage extract (RCE).

Effect of red cabbage extract (RCE; 1.66–13.6 μg/ml) on the rate of Ca2+-induced mitochondrial permeability transition (MPT). Dependences of mitochondrial swelling rate v (ΔD520/min) on RCE concentration. Energised by 5 mM succinate isolated rat liver mitochondria (0.5 mg protein/ml) were incubated in the medium containing 0.125 M sucrose, 0.06 M KCl, 0.02 M Tris-HCl and 0.001 M KH2PO4, pH 7.2 (ethylene glycol tetraacetic acid [EGTA]-free medium), with or without RCE at 25 °C. The reaction was started by addition of 60 μM of Ca2+ ions. The rate of the MPT pore formation was determined from the changes in the absorbance of mitochondrial suspension at 520 nm. Significant difference (p < 0.05): * vs. that of the control, # vs. that in the presence of 60 μM Ca2+.

Blood immunological parameters in alcohol-administered rats treated with red cabbage extract (RCE) anthocyanins_

Parameters	Control	ASH	ASH + 11 mg/kg RCE	ASH + 22 mg/kg RCE
Nitro blue tetrazolium test, %	21.4 ± 1.2	7.7 ± 2.4^a	38.3 ± 6.9^b	38.3 ± 6.5^b
Phagocytic index, %	55.4 ± 3.5	21.1 ± 1.5^a	39.9 ± 3.5^b	37.1 ± 4.6^b

Morphometric evaluation of hepatic damage and blood serum triglycerides, bilirubin levels, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase activities and liver triglycerides content during long-term alcohol administration and red cabbage extract (RCE) anthocyanins treatment_

Parameters	Control	ASH	ASH + 11 mg/kg RCE	ASH + 22 mg/kg RCE
Square of sudanophilic area, % of the slide square	2.2 ± 0.2	16.8 ± 1.8^a	13.8 ± 1.5^a	12.6 ± 1.4^ab
AST, U/l	67.0 ± 3.5	170.1 ± 2.6^a	165.4 ± 3.5^a	165.1 ± 3.1^a
ALT, U/l	53.0 ± 2.2	78.8 ± 2.1^a	63.2 ± 3.4^ab	56.4 ± 2.4^b
Alkaline phosphatase, U/l	185.9 ± 13.7	406.9 ± 41.4^a	300.4 ± 19.2^ab	271.0 ± 14.6^ab
Bilirubin, total, μmol/l	4.0 ± 0.2	6.0 ± 0.3^a	4.6 ± 0.4^b	4.6 ± 0.3^b
Bilirubin, bound, μmol/l	1.9 ± 0.2	2.7 ± 0.3^a	1.7 ± 0.2^b	1.6 ± 0.1^b
Serum triglycerides, mmol/l	1.8 ± 0.2	2.8 ± 0.2^a	2.0 ± 0.1^b	1.9 ± 0.2^b
Liver triglycerides, mg/g tissue	16.8 ± 0.9	39.1 ± 2.7^a	35.5 ± 1.9^ab	30.3 ± 1.9^ab

Rat blood cytokines tumor necrosis factor α (TNFα), transforming growth factor β (TGFβ), interleukin-6 (IL-6), and leptin levels during alcohol administration and red cabbage extract (RCE) anthocyanins treatment_

Parameters	Control	ASH	ASH + 11 mg/kg RCE	ASH + 22 mg/kg RCE
TNFα, pg/ml	8.4 ± 0.8	49.9 ± 3.8^a	50.8 ± 4.7^a	35.6 ± 3.2^ab
TGFβ, ng/ml	57.3 ± 7.6	141.9 ± 21.0^a	152.9 ± 36.2^a	125.0 ± 25.3^a
IL-6, pg/ml	50.4 ± 4.9	147.0 ± 13.2^a	107.3 ± 12.5^ab	80.9 ± 6.2^ab
Leptin, ng/ml	1.1 ± 0.1	1.9 ± 0.1^a	1.9 ± 0.1^a	1.3 ± 0.2^b

Respiratory parameters of rat liver mitochondria during alcohol administration and red cabbage extract (RCE) anthocyanins treatment_

Parameters	Control	ASH	ASH + 11 mg/kg RCE	ASH + 22 mg/kg RCE
V3, ngatom O/min/mg protein	94.8 ± 8.9	59.7 ± 6.2^a	104.7 ± 9.2^b	110.8 ± 7.2^b
V4, ngatom O/min/mg protein	41.4 ± 4.7	29.7 ± 4.8^a	45.2 ± 4.1^b	39.5 ± 4.5^b
Respiratory control ratio (RCR)	2.3 ± 0.3	2.0 ± 0.2	2.1 ± 0.3	2.2 ± 0.2
ADP/O	1.6 ± 0.2	1.1 ± 0.2^a	1.7 ± 0.3^b	1.8 ± 0.2^b

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DOI: https://doi.org/10.2478/afpuc-2022-0014 | Journal eISSN: 2453-6725

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Language: English

Page range: 5 - 16

Submitted on: Nov 3, 2021

Accepted on: Feb 24, 2022

Published on: Aug 23, 2022

Published by: Comenius University in Bratislava, Faculty of Pharmacy

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

red cabbage anthocyanins,

liver,

alcoholic steatohepatitis,

mitochondrial respiration

Related subjects:

Pharmacy,

Pharmacy, other

© 2022 V. Buko, E. Belonovskaya, T. Kavalenia, T. Ilyich, S. Kirko, I. Kuzmitskaya, V. Moroz, E. Lapshina, A. Romanchuk, I. Zavodnik, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 69 (2022): Issue 2 (August 2022)

Anthocyanin-Rich Extract of Red Cabbage Attenuates Advanced Alcohol Hepatotoxicity in Rats in Association with Mitochondrial Activity Modulation

Figures & Tables

Figure 1

Figure 2

Figure 3

Figure 4

Blood immunological parameters in alcohol-administered rats treated with red cabbage extract (RCE) anthocyanins_

Rat blood cytokines tumor necrosis factor α (TNFα), transforming growth factor β (TGFβ), interleukin-6 (IL-6), and leptin levels during alcohol administration and red cabbage extract (RCE) anthocyanins treatment_

Respiratory parameters of rat liver mitochondria during alcohol administration and red cabbage extract (RCE) anthocyanins treatment_

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