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Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2 Cover

Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2

European Pharmaceutical Journal
Volume 69 (2022): Issue s1 (July 2022)
By: Mykhailo Huniadi,  Simon Salanci,  Jan Sykora,  Slavomir Kurhajec and  Marek Sarissky  
Open Access
|Jun 2022

Figures & Tables

Figure 1

Illustrative bivariate dotplots showing the results of cySAP (A) and cyEAT-2 (B) analysis by flow cytometry: control (unstained) cells (grey), T cells (light green), B cells (dark green), NK cells (blue), monocytes (yellow), neutrophils (violet) and eosinophils (pink).
Illustrative bivariate dotplots showing the results of cySAP (A) and cyEAT-2 (B) analysis by flow cytometry: control (unstained) cells (grey), T cells (light green), B cells (dark green), NK cells (blue), monocytes (yellow), neutrophils (violet) and eosinophils (pink).

Flow cytometric analysis of SAP and EAT-2 adaptor proteins in lymphocytes_

MFI control (unstained) cellsMFI stained cells
MeanSDCVMeanSDCV
cySAPT cellsFix&Perm3.11.239.64.81.632.7
TFBS2.71.347.917.111.567.5
IntraPrep3.80.922.623.910.343.1
PerFix4.92.041.373.954.273.3
B cellsFix&Perm1.60.636.94.01.947.3
TFBS1.10.215.210.07.070.3
IntraPrep3.51.026.815.712.277.9
PerFix2.70.727.128.927.394.8
NK cellsFix&Perm1.80.630.94.52.454.0
TFBS1.20.432.318.99.148.1
IntraPrep4.00.819.522.69.542.1
PerFix2.40.623.449.922.645.4
cyEAT-2T cellsFix&PermNDNDNDNDNDND
TFBSNDNDNDNDNDND
IntraPrep4.00.921.783.946.355.1
PerFix5.82.136.8192.564.633.6
B cellsFix&PermNDNDNDNDNDND
TFBSNDNDNDNDNDND
IntraPrep3.70.924.263.137.359.1
PerFix3.71.335.2189.060.932.2
NK cellsFix&PermNDNDNDNDNDND
TFBSNDNDNDNDNDND
IntraPrep4.21.024.0211.2178.684.6
PerFix3.10.723.1537.7227.342.3

Panel of antibodies_

Antibodies and fluorochromes
Tube No.PBKOFITCPEECDPC5.5PC7APCAPC-A700APC-A750
1CD4 + CD20CD45-cyCTRL-CD5CD10CD3CD19CD8
2CD4 + CD20CD45-cySAP-CD5CD10CD3CD19CD8
3CD4 + CD20CD45-cyCTRL +sec.Ab-CD5CD10CD3CD19CD8
4CD4 + CD20CD45-cyEAT-2 +sec.Ab-CD5CD10CD3CD19CD8
DOI: https://doi.org/10.2478/afpuc-2022-0007 | Journal eISSN: 2453-6725
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Language: English
Page range: 87 - 89
Submitted on: Apr 21, 2022
Accepted on: May 10, 2022
Published on: Jun 18, 2022
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
SLAMF receptors,
SAP,
EAT-2,
lymphocytes,
flow cytometry
Related subjects:
Pharmacy,
Pharmacy, other

© 2022 Mykhailo Huniadi, Simon Salanci, Jan Sykora, Slavomir Kurhajec, Marek Sarissky, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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