Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2

Mykhailo Huniadi; Simon Salanci; Jan Sykora; Slavomir Kurhajec; Marek Sarissky

doi:10.2478/afpuc-2022-0007

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Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2

European Pharmaceutical Journal

Volume 69 (2022): Issue s1 (July 2022)

By: Mykhailo Huniadi, Simon Salanci, Jan Sykora, Slavomir Kurhajec and Marek Sarissky

Open Access

|Jun 2022

Abstract

SAP and EAT-2 are intracellular adaptor proteins that link SLAMF receptors to downstream intracellular signalling pathways. Their expression and functions vary among different types of hematopoietic cells. The roles of SAP and EAT-2 in haematological malignancies are poorly understood. Here, we attempted to optimise a method for the detection of intracellular SAP and EAT-2 by flow cytometry. We found that using PerFix EXPOSE kit for cell fixation and permeabilization ensures optimal results in terms of staining intensity and signal-to-noise ratio.

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Articles in this issue

DOI: https://doi.org/10.2478/afpuc-2022-0007 | Journal eISSN: 2453-6725

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Language: English

Page range: 87 - 89

Submitted on: Apr 21, 2022

Accepted on: May 10, 2022

Published on: Jun 18, 2022

Published by: Comenius University in Bratislava, Faculty of Pharmacy

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

SLAMF receptors,

SAP,

EAT-2,

Related subjects:

© 2022 Mykhailo Huniadi, Simon Salanci, Jan Sykora, Slavomir Kurhajec, Marek Sarissky, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 69 (2022): Issue s1 (July 2022)