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Study of qualitative and quantitative content of amino acids in pumpkin seeds for further standardization of the herbal drug Cover

Study of qualitative and quantitative content of amino acids in pumpkin seeds for further standardization of the herbal drug

European Pharmaceutical Journal
Volume 67 (2020): Issue 1 (January 2020)
By: E.E. Kotova,  S.A. Kotov,  T.M. Gontova and  A.G. Kotov  
Open Access
|Nov 2020

Figures & Tables

Figure 1

Pumpkin seeds: dried drug substance
Pumpkin seeds: dried drug substance

Figure 2

The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 1,2,3,4, test solutions obtained from different series (RS 799, 800, 801, 802); RS 1, solution of arginine (zone with lower Rf) and glycine (zone with higher Rf); RS 2, solution of alanine (zone with lower Rf) and leucine (zone with higher Rf); RS 3, solution of aspartic acid (zone with lower Rf) and solution of glutamic acid (zone with higher Rf)
The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 1,2,3,4, test solutions obtained from different series (RS 799, 800, 801, 802); RS 1, solution of arginine (zone with lower Rf) and glycine (zone with higher Rf); RS 2, solution of alanine (zone with lower Rf) and leucine (zone with higher Rf); RS 3, solution of aspartic acid (zone with lower Rf) and solution of glutamic acid (zone with higher Rf)

Figure 3

The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 5,6,7, test solutions obtained from different series (RS 803, 804, 805); RS 4, solution of histidine, aspartic acid, glutamic acid, valine (in order of increasing Rf); RS 5, solution of arginine, glycine, alanine, and leucine (in order of increasing Rf)
The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 5,6,7, test solutions obtained from different series (RS 803, 804, 805); RS 4, solution of histidine, aspartic acid, glutamic acid, valine (in order of increasing Rf); RS 5, solution of arginine, glycine, alanine, and leucine (in order of increasing Rf)

Figure 4

Typical absorption spectra obtained by assay amino acids content: 1,2, spectra of the test solutions obtained from the series of pumpkin seeds; RS, spectrum of solution of glutamic acid
Typical absorption spectra obtained by assay amino acids content: 1,2, spectra of the test solutions obtained from the series of pumpkin seeds; RS, spectrum of solution of glutamic acid

Results of amino acids assay expressed as glutamic acid and fat-free raw material in the different samples of pumpkin seeds

RS (batches of pumpkin seeds)The content of the amino acids expressed as glutamic acid
7992.0 ± 0.05
8002.1 ± 0.03
8011.9 ± 0.04
8021.8 ± 0.05
8032.5± 0.07
8041.7 ± 0.03
8051.8 ± 0.04
DOI: https://doi.org/10.2478/afpuc-2020-0001 | Journal eISSN: 2453-6725
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Language: English
Page range: 27 - 32
Submitted on: Sep 5, 2019
Accepted on: Nov 27, 2019
Published on: Nov 18, 2020
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
pumpkin seeds,
amino acids,
TLC method,
UV-vis spectrophotometry method,
standardization,
SPhU
Related subjects:
Pharmacy,
Pharmacy, other

© 2020 E.E. Kotova, S.A. Kotov, T.M. Gontova, A.G. Kotov, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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