Application of selective polymeric sorbents for simple coumarins extraction from deodorant samples

Coumarins (2H-1-benzopyran-2-coumarin derivatives) are derivatives of cinnamic acid naturally occurring in many plants, fungi, and fruits. They are used as ingredients in cosmetics to enhance the aroma and other biological effects. In this work, cosmetic samples (deodorants) were treated by solid phase extraction prior to high performance liquid chromatography determination of coumarins. Traditional sorbent (C18) and selective polymer-based sorbents (laboratory prepared and commercial) were used for solid phase extraction. Recovery values were above 85 % (RSDs below 6 %) except for esculin, where the recovery was lower. Core-shell column of C18 type and gradient of mobile phase methanol—1 % acetic acid were used for high performance liquid chromatography analysis of extracts. Limits of quantitation were 0.5 µg mL⁻¹ for coumarin (ultraviolet detection) and below 12 ng mL⁻¹ for esculin, umbelliferonene, scoparone, 4-methylumbelliferone, herniarin (fluorescence detection). In the tested samples, no coumarins were detected.