Pharmacokinetics study of a supersaturatable self-microemulsifying drug delivery system for ellagic acid by UHPLC-Q-TOF-MS

Wang, Jinglong; Fan, Liqun; Zhang, Lihua; Zheng, Dandan; Wang, Yingzi; Sun, Xiao; Ji, Yonghui

doi:10.2478/acph-2021-0031

Abstract

To evaluate the bioavailability of ellagic acid loaded super-saturatable self-microemulsifying drug delivery system (S-SMEDDS), its pharmacokinetic properties were studied in rats with an ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry. The plasma samples were treated by solid-phase extraction method, and gallic acid was used as the internal standard when determining the concentration of ellagic acid. Results showed that the established analytical method was sensitive and accurate, which is applicable to the pharmacokinetic study of ellagic acid. The drug was found to be absorbed rapidly in vivo, and the plasma concentration-time curve showed double peaks, indicating that ellagic acid were reabsorbed by entero-hepatic circulation after oral administration. Compared with ellagic acid suspension, the apparent clearance of ellagic acid-loaded S-SMEDDS and SMEDDS reduced significantly, and the AUC_0~t of them were 4.7 and 5.8-fold increase, respectively. Therefore, the bioavailability of ellagic acid-loaded S-SMEDDS was higher than that of the suspension and SMEDDS.

References

1. G. Quan, B. Niu, V. Singh, Y. Zhou, C. Wu, X. Pan and C. Wu, Supersaturable solid self-microemulsifying drug delivery system: precipitation inhibition and bioavailability enhancement, Int. J. Nanomed.12 (2017) 8801–8811; https://doi.org/10.2147/IJN.S14971710.2147/IJN.S149717
Search in Google Scholar Back to article
2. N. T. Tung, C. S. Tran, H. A. Nguyen, T. D. Nguyen, S. C. Chi, D. V. Pham, Q. D. Bui and X. H. Ho, Formulation and biopharmaceutical evaluation of supersaturatable self-nanoemulsifying drug delivery systems containing silymarin, Int. J. Pharm.555 (2019) 63–76; https://doi.org/10.1016/j.ijpharm.2018.11.03610.1016/j.ijpharm.2018.11.036
Search in Google Scholar Back to article
3. Z. Q. Chen, Y. Liu, J. H. Zhao, L. Wang and N. P. Feng, Improved oral bioavailability of poorly water-soluble indirubin by a supersaturatable self-microemulsifying drug delivery system, Int. J. Nanomed.7 (2012) 1115–1125; https://doi.org/10.2147/IJN.S2876110.2147/IJN.S28761
Search in Google Scholar Back to article
4. J. M. Landete, Ellagitannins, ellagic acid and their derived metabolites: A review about source, metabolism, functions and health, Food Res. Int.44 (2011) 1150–1160; https://doi.org/10.1016/j.foodres.2011.04.02710.1016/j.foodres.2011.04.027
Search in Google Scholar Back to article
5. F. Lei, D. M. Xing, L. Xiang, Y. N. Zhao, W. Wang, L. J. Zhang and L. J. Du, Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract, J. Chromatogr. B796 (2003) 189–194; https://doi.org/10.1016/S1570-0232(03)00610-X10.1016/S1570-0232(03)00610-X
Search in Google Scholar Back to article
6. A. W. R. Hamad, W. M. Al-Momani, S. Janakat and S. A. Oran, Bioavailability of ellagic acid after single dose administration using HPLC, Par. J. Stat.8 (2009) 1661–1664; https://DOI:10.3923/pjn.2009.1661.166410.3923/pjn.2009.1661.1664
Search in Google Scholar Back to article
7. L. Yan, P. Yin, C. Ma and Y. Liu, Method development and validation for pharmacokinetic and tissue distributions of ellagic acid using ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), Molecules19 (2014) 18923–18935; https://doi:10.3390/molecules19111892310.3390/molecules191118923627145925412040
Search in Google Scholar Back to article
8. J. Wang, D. Zheng, Y. Wang, C. Zhang and X. Sun, Pharmacokinetics study of Erhuang decoction extracts in rats by HPLC-MS/MS, J. Chromatogr. B1059 (2017) 35–42; https://doi.org/10.1016/j.jchromb.2017.05.01910.1016/j.jchromb.2017.05.01928570935
Search in Google Scholar Back to article
9. G. R. Valicherla, M. Riyazuddin, S. Shahi, A. P. Gupta, A. A. Syed, A. Husain and J. R. Gayen, LCESI-MS/MS assay development and validation of a novel antidiabetic peptide PSTi8 in mice plasma using SPE: An application to pharmacokinetics, J. Pharm. Biomed. Anal.180 (2019) 113074; https://doi.org/10.1016/j.jpba.2019.11307410.1016/j.jpba.2019.11307431891874
Search in Google Scholar Back to article
10. V. Ferrone, M. Carlucci, P. Palumbo and G. Carlucci, Bioanalytical method development for quantification of ulifloxacin, fenbufen and felbinac in rat plasma by solid-phase extraction (SPE) and HPLC with PDA detection, J. Pharm. Biomed. Anal.123 (2016) 205–212; https://doi.org/10.1016/j.jpba.2016.01.06210.1016/j.jpba.2016.01.06226898973
Search in Google Scholar Back to article
11. D. Zheng, C. Lv, X. Sun, J. Wang and Z. Zhao, Preparation of a supersaturatable self-microemulsion as drug delivery system for ellagic acid and evaluation of its antioxidant activities, J. Drug Deliv. Sci. Technol.53 (2019) 101209; https://doi.org/10.1016/j.jddst.2019.10120910.1016/j.jddst.2019.101209
Search in Google Scholar Back to article
12. G. Z. Pei, W. Chen, H. Zhang and G. H. Li, Pharmacokinetics of ellagic acid tablets in rabbits, Chin. J. Exp. Tradit. Med. Formulae18 (2012) 136–138; https://doi.org/10.13422/j.cnki.syfjx.2012.12.047
Search in Google Scholar Back to article
13. C. Sun, Y. Gui, R. Hu, J. Chen, B. Wang, Y. Guo, W. Lu, X. Nie, Q. Shen, S. Gao and W. Fang, Preparation and Pharmacokinetics Evaluation of Solid Self-Microemulsifying Drug Delivery System (SSMEDDS) of Osthole, AAPS PharmSciTech19 (2018) 2301–2310; https://doi.org/10.1208/s12249-018-1067-310.1208/s12249-018-1067-329845504
Search in Google Scholar Back to article
14. J. Jinno, N. Kamada, M. Miyake, K. Yamada, T. Mukai, M. Odomi, H. Toguchi, G. G. Liversidge, K. Higaki and T. Kimura, Effect of particle size reduction on dissolution and oral absorption of a poorly water-soluble drug, cilostazol, in beagle dogs, J. Controll. Release111 (2006) 56–64; https://doi.org/10.1016/j.jconrel.2005.11.01310.1016/j.jconrel.2005.11.01316410029
Search in Google Scholar Back to article
15. H. Mu, R. Holm and A. Müllertz, Lipid-based formulations for oral administration of poorly water-soluble drugs, Int. J. Pharm.453 (2013) 215–224; https://doi.org/10.1016/j.ijpharm.2013.03.05410.1016/j.ijpharm.2013.03.05423578826
Search in Google Scholar Back to article

Pharmacokinetics study of a supersaturatable self-microemulsifying drug delivery system for ellagic acid by UHPLC-Q-TOF-MS

Abstract

Paradigm

My account