Influence of coliform bacteria infection on intestinal goblet cells secretory activity of germ-free piglets

Toth Stefan; Holodova Monika; Zeidan Deema; Hartel Patrick; Fagova Zuzana; Curgali Kristina; Mechirova Eva; Maretta Milan; Nemcova Radomira; Gancarčikova Sona; Polak Stefan; Dankova Marianna

doi:10.2478/acm-2022-0008

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Influence of coliform bacteria infection on intestinal goblet cells secretory activity of germ-free piglets

Acta Medica Martiniana

Volume 22 (2022): Issue 2 (August 2022)

By: Toth Stefan, Holodova Monika, Zeidan Deema, Hartel Patrick, Fagova Zuzana, Curgali Kristina, Mechirova Eva, Maretta Milan, Nemcova Radomira, Gancarčikova Sona, Polak Stefan and Dankova Marianna

Open Access

|Aug 2022

Abstract

Recently, influence of bacteria colonization on development and maturation of gut wall is getting more into the focus of gastrointestinal research. For years, the main interest and research were aimed to the development and maturation of gut wall and its functional properties in normal conditions, less attention has been paid on the germ-free animals. Germ-free (GF) piglets have clear microbiological background and are reared in sterile environment. GF piglets are regarded as clinically relevant models for studying of human diseases, as these piglets’ manifest similar clinical symptoms to humans. In this study we briefly summarised the main characteristics in the distribution of goblet cells in the wall of jejunum and colon of GF piglets as healthy control (HC) group and piglets, which were experimentally infected by E. coli O149:K88 as ECK group. Neutral mucins were stained with periodic acid-Shiff (PAS) whereas acidic mucins are stained with Alcian blue. Numbers of goblet cells containing total acidic mucins in both, the jejunum and colon, differed significantly between HC and ECK piglets and in the colon, a similar trend was also observed. In the ECK piglets, jejunal goblet cells exhibited decrease in neutral mucins. This change in mucin profile in response to bacterial colonization suggests a potential role as a protective mechanism against pathogenic invasion of the intestinal mucosa during of gut mucosa development in piglets.

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Articles in this issue

DOI: https://doi.org/10.2478/acm-2022-0008 | Journal eISSN: 1338-4139 (formerly 1335-8421) | Journal ISSN: 1335-8421

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Language: English

Page range: 62 - 69

Submitted on: Jun 7, 2022

Accepted on: Jun 24, 2022

Published on: Aug 23, 2022

Published by: Sciendo

In partnership with: Paradigm Publishing Services

Publication frequency: 3 issues per year

Keywords:

germ free environment,

piglet,

Related subjects:

© 2022 Toth Stefan, Holodova Monika, Zeidan Deema, Hartel Patrick, Fagova Zuzana, Curgali Kristina, Mechirova Eva, Maretta Milan, Nemcova Radomira, Gancarčikova Sona, Polak Stefan, Dankova Marianna, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 22 (2022): Issue 2 (August 2022)