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Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit Cover

Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit

Medical Journal of Cell Biology
Volume 9 (2021): Issue 1 (March 2021)
By: Zeinab Shafiei Seifabadi,  Fatemeh Rezaei-Tazangi,  Nastaran Azarbarz,  Darioush Bijan Nejad,  Javad Mohammadiasl,  Hanieh Darabi and  Seyed Pezhmanlarki-Tork  
Open Access
|Mar 2021

Figures & Tables

Figure 1

The Morphology of Wharton's Jelly-Derived MesenchymalStem Cells at (A) Primary Culture and (B) 3rd Passage

Figure 2

The Frequency of the Positive Cells for CD Markers by Flow Cytometry. The HUCWJ MSCs expressed CD44 and CD90, but they expressed CD34 and CD144 with less frequency

Figure 3

Osteogenic differentiation was confirmed by staining of Calcium deposits with Alizarin Red/S (A). Oil Red showed the presence of lipid droplets in the cells cultured in adipogenic medium (B). Magnification: 400 X

Figure 4

Morphology of the cells within the alginate beads. A. Alginate/Cacl2 B. Alginate/Bacl2. Magnification: 250 X

Figure 5

Cell viability within capsules after 24, 48 and 72 h. Values are expressed as mean ± SD. *; P < 0.05, **; P < 0.01, #; P < 0.05. * And # symbols indicate comparison to the control and Bacl2 groups
DOI: https://doi.org/10.2478/acb-2021-0007 | Journal eISSN: 2544-3577
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Language: English
Page range: 42 - 47
Submitted on: Feb 22, 2021
|
Accepted on: Mar 13, 2021
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Published on: Mar 30, 2021
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Wharton's jelly,
mesenchymal stem cells,
alginate,
viability
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2021 Zeinab Shafiei Seifabadi, Fatemeh Rezaei-Tazangi, Nastaran Azarbarz, Darioush Bijan Nejad, Javad Mohammadiasl, Hanieh Darabi, Seyed Pezhmanlarki-Tork, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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