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Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit Cover

Assessment of viability of wharton's jelly mesenchymal stem cells encapsulated in alginate scaffold by WST-8 assay kit

Medical Journal of Cell Biology
Volume 9 (2021): Issue 1 (March 2021)
By: Zeinab Shafiei Seifabadi,  Fatemeh Rezaei-Tazangi,  Nastaran Azarbarz,  Darioush Bijan Nejad,  Javad Mohammadiasl,  Hanieh Darabi and  Seyed Pezhmanlarki-Tork  
Open Access
|Mar 2021

Figures & Tables

Figure 1

The Morphology of Wharton's Jelly-Derived MesenchymalStem Cells at (A) Primary Culture and (B) 3rd Passage
The Morphology of Wharton's Jelly-Derived MesenchymalStem Cells at (A) Primary Culture and (B) 3rd Passage

Figure 2

The Frequency of the Positive Cells for CD Markers by Flow Cytometry. The HUCWJ MSCs expressed CD44 and CD90, but they expressed CD34 and CD144 with less frequency
The Frequency of the Positive Cells for CD Markers by Flow Cytometry. The HUCWJ MSCs expressed CD44 and CD90, but they expressed CD34 and CD144 with less frequency

Figure 3

Osteogenic differentiation was confirmed by staining of Calcium deposits with Alizarin Red/S (A). Oil Red showed the presence of lipid droplets in the cells cultured in adipogenic medium (B). Magnification: 400 X
Osteogenic differentiation was confirmed by staining of Calcium deposits with Alizarin Red/S (A). Oil Red showed the presence of lipid droplets in the cells cultured in adipogenic medium (B). Magnification: 400 X

Figure 4

Morphology of the cells within the alginate beads. A. Alginate/Cacl2 B. Alginate/Bacl2. Magnification: 250 X
Morphology of the cells within the alginate beads. A. Alginate/Cacl2 B. Alginate/Bacl2. Magnification: 250 X

Figure 5

Cell viability within capsules after 24, 48 and 72 h. Values are expressed as mean ± SD. *; P < 0.05, **; P < 0.01, #; P < 0.05. * And # symbols indicate comparison to the control and Bacl2 groups
Cell viability within capsules after 24, 48 and 72 h. Values are expressed as mean ± SD. *; P < 0.05, **; P < 0.01, #; P < 0.05. * And # symbols indicate comparison to the control and Bacl2 groups
DOI: https://doi.org/10.2478/acb-2021-0007 | Journal eISSN: 2544-3577
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Language: English
Page range: 42 - 47
Submitted on: Feb 22, 2021
Accepted on: Mar 13, 2021
Published on: Mar 30, 2021
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Wharton's jelly,
mesenchymal stem cells,
alginate,
viability
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2021 Zeinab Shafiei Seifabadi, Fatemeh Rezaei-Tazangi, Nastaran Azarbarz, Darioush Bijan Nejad, Javad Mohammadiasl, Hanieh Darabi, Seyed Pezhmanlarki-Tork, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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