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Telomerase Activity and Myogenesis Ability as an Indicator of Cultured Turkey Satellite Cell Ability for In Vitro Meat Production Cover

Telomerase Activity and Myogenesis Ability as an Indicator of Cultured Turkey Satellite Cell Ability for In Vitro Meat Production

Medical Journal of Cell Biology
Volume 9 (2021): Issue 1 (March 2021)
By: Afsaneh Golkar Narenji,  James N. Petitte,  Magdalena Kulus,  Katarzyna Stefańska,  Joanna Perek,  Jakub Kulus,  Maria Wieczorkiewicz,  Maurycy Jankowski,  Artur Bryja,  Rut Bryl,  Paweł Antosik,  Dorota Bukowska,  Jędrzej M. Jaśkowki,  Kornel Ratajczak and  Paul Mozdziak  
Open Access
|Mar 2021

Figures & Tables

Figure 1

Detection of telomerase in the cultured cells. A: Primer dimer control, B: Cultured Turkey cells, C*: Heat treated cultured turkey cells, D: Rat myoblast cells, E*: Heat treated rat myoblast cells. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability

Figure 2

Telomerase detection in undifferentiated versus differentiated cells. A: Primer dimer Control, B: kit positive control, C*: Heat treated kit positive control, D:Undifferentiated cells E*: Heat treated undifferentiated cells, F: Differentiated cells, G*: Heat treated differentiated cells. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability

Figure 3

Telomerase detection in suspended myoblasts. A:Primer dimer control, B:kit positive control, C: Heat treated kit positive control, D: Short-term turkey cell culture in suspension, E: Heat treated short-term turkey cell culture in suspension, F:Long-term Turkey cell culture in suspension, G: Heat treated Long-term Turkey cell culture in suspension. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability

Figure 4

Gene expression in differentiated versus undifferentiated cells. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as myotube cell versus myoblast cell values

Figure 5

Gene expression in long-term suspension culture versus adherent cells. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as long-term suspended culture versus adherent cells FC values

Figure 6

Gene expression in short-term (3 month) versus long-term (12 month) cell culture in suspension. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as short vs long-term suspended culture cell values

Primer sequences were used for the amplification of each gene

Turkey MyoD1Forward: GCTCCAAGACCTGCCTTCTA
Reverse: AAACATTGGCCATTTCCCCG
Turkey MyogeninForward: ACCGCAACCTCCACTCTCTC
Reverse: GCCAGCTCAGTTTTGGACCC
Turkey TERT 1Forward: ACTGCTGAAGACAGTGACGGA
Reverse: AACCTTCATCGCTTAGTCCAGT
Turkey TERT 2Forward: GCTGGCAAGTCTCTGTGACTCTT
Reverse: GGAGAGGGAGCTGCTCAGAAC
Turkey MYF5Forward: AGCTGCCAGTTCTCCCCATC
Reverse: GTGCTCCTCTTCCTCAGGGC
Turkey MRF4Forward: TCCGACTTCCTGAGCACCTG
Reverse: TTTCTCCACCGCCTCTTCCG
Turkey Pax7Forward: TGGCGGATGTGGTTGGGTAA
Reverse: TAGAAGTGTCCACCCCGCTG
Turkey β-actinForward: TACTCCCACAGCCAGCCATG
Reverse: TGGGGTACTTCAGGGTCAGGA
Turkey DyskerinForward: GTTAGCGACTACCCCCGACC
Reverse: GTGGAGACCCCGTTCCTACG
Turkey ALDH1Forward: AATCGCTCCCTTGCTGTTGA
Reverse: AATCGCTCCCTTGCTGTTGA
Turkey ALDH2Forward: GCTAGTCGTAAAGACCCCCG
Reverse: TCTGGCACGGAAAATGCTCA
Turkey NanogForward: CAGAGCAGAAAACGCCCAGC
Reverse: CCTTGTCCCACTCTCACCTTTA
Turkey GAPDHForward: TACTCCCACAGCCAGCCATG
Reverse: ACGGCAGGTCAGGTCAACAA
DOI: https://doi.org/10.2478/acb-2021-0004 | Journal eISSN: 2544-3577
Journal RSS Feed
Language: English
Page range: 19 - 26
Submitted on: Feb 3, 2021
|
Accepted on: Mar 6, 2021
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Published on: Mar 30, 2021
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
telomerase,
myoblasts,
myogenesis,
2D cell culture,
long-term suspension cell culture,
in vitro meat production
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2021 Afsaneh Golkar Narenji, James N. Petitte, Magdalena Kulus, Katarzyna Stefańska, Joanna Perek, Jakub Kulus, Maria Wieczorkiewicz, Maurycy Jankowski, Artur Bryja, Rut Bryl, Paweł Antosik, Dorota Bukowska, Jędrzej M. Jaśkowki, Kornel Ratajczak, Paul Mozdziak, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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