Table 1
Oligonucleotide sequences of primers used for RT-qPCR analysis
| GENE | PRIMER SEQUENCE (5’–3’) | PRODUCT SIZE (BP) |
|---|---|---|
| IL1β | F GGGCCTCAAGGAAAAGAATC R TTCTGCTTGAGAGGTGCTGA | 205 |
| IL6 | F ACAACCTGAACCTTCCAAAGA R ACAACCTGAACCTTCCAAAGA | 179 |
| TNFα | F AGCCCATGTTGTAGCAAACC R AGCCCATGTTGTAGCAAACC | 157 |
| ACTB | F AAAGACCTGTACGCCAACAC R CTCAGGAGGAGCAATGATCTTG | 132 |
| GAPDH | F TCAGCCGCATCTTCTTTTGC R ACGACCAAATCCGTTGACTC | 90 |
| HPRT | F TGGCGTCGTGATTAGTGATG R ACATCTCGAGCAAGACGTTC | 141 |
Figure 1
The change in the expression of IL1B, IL6 and TNFa genes in 48h and 72h in short-term in vitro culture of human ovarian granulosa cells, as compared to the expression in 24 hours of culture