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Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture Cover

Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture

Medical Journal of Cell Biology
Volume 8 (2020): Issue 4 (December 2020)
By: Sandra Kałużna,  Rut Bryl,  Błażej Chermuła,  Rafał Sibiak,  Katarzyna Stefańska,  Wojciech Pieńkowski,  Wiesława Kranc,  Michal Jeseta,  Pavel Ventruba,  Jana Zakova,  Igor Crha,  Leszek Pawelczyk and  Robert Z. Spaczyński  
Open Access
|Dec 2020

Figures & Tables

Figure 1

The change in the expression of IL1B, IL6 and TNFa genes in 48h and 72h in short-term in vitro culture of human ovarian granulosa cells, as compared to the expression in 24 hours of culture
The change in the expression of IL1B, IL6 and TNFa genes in 48h and 72h in short-term in vitro culture of human ovarian granulosa cells, as compared to the expression in 24 hours of culture

Oligonucleotide sequences of primers used for RT-qPCR analysis

GENEPRIMER SEQUENCE (5’–3’)PRODUCT SIZE (BP)
IL1βF GGGCCTCAAGGAAAAGAATCR TTCTGCTTGAGAGGTGCTGA205
IL6F ACAACCTGAACCTTCCAAAGAR ACAACCTGAACCTTCCAAAGA179
TNFαF AGCCCATGTTGTAGCAAACCR AGCCCATGTTGTAGCAAACC157
ACTBF AAAGACCTGTACGCCAACACR CTCAGGAGGAGCAATGATCTTG132
GAPDHF TCAGCCGCATCTTCTTTTGCR ACGACCAAATCCGTTGACTC90
HPRTF TGGCGTCGTGATTAGTGATGR ACATCTCGAGCAAGACGTTC141
DOI: https://doi.org/10.2478/acb-2020-0025 | Journal eISSN: 2544-3577
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Language: English
Page range: 190 - 195
Submitted on: Nov 18, 2020
Accepted on: Dec 21, 2020
Published on: Dec 31, 2020
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
granulosa cells (GCs),
IL1β,
IL6,
i TNFα
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2020 Sandra Kałużna, Rut Bryl, Błażej Chermuła, Rafał Sibiak, Katarzyna Stefańska, Wojciech Pieńkowski, Wiesława Kranc, Michal Jeseta, Pavel Ventruba, Jana Zakova, Igor Crha, Leszek Pawelczyk, Robert Z. Spaczyński, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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