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Study of the expression of genes associated with post-translational changes in histones in the internal thoracic artery and the saphenous vein grafts used in coronary artery bypass grafting procedure Cover

Study of the expression of genes associated with post-translational changes in histones in the internal thoracic artery and the saphenous vein grafts used in coronary artery bypass grafting procedure

Medical Journal of Cell Biology
Volume 8 (2020): Issue 4 (December 2020)
By: Sandra Kałużna,  Mariusz J. Nawrocki,  Rut Bryl,  Katarzyna Stefańska,  Marek Jemielity,  Paul Mozdziak,  Michał Nowicki and  Bartłomiej Perek  
Open Access
|Dec 2020

Figures & Tables

Figure 1

Change in the expression of genes: JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 in the saphenous vein in relation to the expression in the internal thoracic artery - vessels taken from patients who underwent CABG surgery. Total RNA was reverse transcribed and cDNA was analyzed by relative quantification by RT-qPCR. JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 mRNA levels were adjusted for PBGD and ACTB levels
Change in the expression of genes: JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 in the saphenous vein in relation to the expression in the internal thoracic artery - vessels taken from patients who underwent CABG surgery. Total RNA was reverse transcribed and cDNA was analyzed by relative quantification by RT-qPCR. JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 mRNA levels were adjusted for PBGD and ACTB levels

Oligonucleotide sequences of primers used for RT-qPCR analysis

GENEPRIMER SEQUENCE (5’–3’)
JHDM1DF TCCCTTCACCTACATTTTCTGR TGCCTGCCTCGCCACATC
PHF8F CCCTCGCCATCATTCACTGR TTCTCCCTCTTCCCGCTGT
HDAC1F GAGACGGGATTGATGACGAR TGAGCCACACTGTAAGACC
HDAC2F GAAGCCTCATAGAATCCR CTCCAACATTAAATCTCTG
HDAC3F GATGACCAGACTTACAAGR GACATATTCAACGCATTC
PBGDF GCCAAGGACCAGGACATCR TCAGGTACAGTTGCCCAT
ACTBF GCACCACACCTTCTACAATGAR ATAGCACAGCCTGGATAGC
DOI: https://doi.org/10.2478/acb-2020-0024 | Journal eISSN: 2544-3577
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Language: English
Page range: 183 - 189
Submitted on: Nov 9, 2020
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Accepted on: Dec 19, 2020
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Published on: Dec 31, 2020
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
coronary artery bypass grafting,
internal thoracic artery,
saphenous vein,
post-translational changes in histones,
JHDM1D,
PHF8,
HDAC1,
HDAC2,
HDAC3
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2020 Sandra Kałużna, Mariusz J. Nawrocki, Rut Bryl, Katarzyna Stefańska, Marek Jemielity, Paul Mozdziak, Michał Nowicki, Bartłomiej Perek, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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