Table 1
Oligonucleotide sequences of primers used for RT-qPCR analysis
| GENE | PRIMER SEQUENCE (5’–3’) |
|---|---|
| JHDM1D | F TCCCTTCACCTACATTTTCTG R TGCCTGCCTCGCCACATC |
| PHF8 | F CCCTCGCCATCATTCACTG R TTCTCCCTCTTCCCGCTGT |
| HDAC1 | F GAGACGGGATTGATGACGA R TGAGCCACACTGTAAGACC |
| HDAC2 | F GAAGCCTCATAGAATCC R CTCCAACATTAAATCTCTG |
| HDAC3 | F GATGACCAGACTTACAAG R GACATATTCAACGCATTC |
| PBGD | F GCCAAGGACCAGGACATC R TCAGGTACAGTTGCCCAT |
| ACTB | F GCACCACACCTTCTACAATGA R ATAGCACAGCCTGGATAGC |
Figure 1
Change in the expression of genes: JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 in the saphenous vein in relation to the expression in the internal thoracic artery - vessels taken from patients who underwent CABG surgery. Total RNA was reverse transcribed and cDNA was analyzed by relative quantification by RT-qPCR. JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 mRNA levels were adjusted for PBGD and ACTB levels