Figure 1
Demonstration of efficiency of the separation methods_ Ability of the separation methods to reduce the number of spermatozoa with fragmented DNA was evaluated, the initial DFI value of the unprocessed ejaculate is considered 100%
| PATIENT | DFI (%; RELATIVE VALUE) | DIFFERENCES BETWEEN SWIM-UP AND FERTILE | ||
|---|---|---|---|---|
| SEMEN | SWIM-UP | FERTILE | ||
| 1 | 100 | 55.0 | 50.7 | 4.3 |
| 2 | 100 | 68.0 | 66.0 | 2.0 |
| 3 | 100 | 42.8 | 26.2 | 16.0 |
| 4 | 100 | 69.0 | 41.3 | 27.7 |
| 5 | 100 | 81.0 | 62.5 | 18.5 |
| 6 | 100 | 15.4 | 6.3 | 9.1 |
| 7 | 100 | 55.9 | 46.8 | 9.1 |
| 8 | 100 | 28.1 | 21.3 | 6.8 |
| 9 | 100 | 32.6 | 25.5 | 7.1 |
| 10 | 100 | 37.5 | 33.7 | 3.8 |
| Ø | 100 | 48.5 | 38.0 | 10.5 |
DFI values of all patients before and after separation by swim-up and FERTILE method
| PATIENT | DFI (%; ABSOLUTE VALUE) | DIFFERENCES BETWEEN SWIM-UP AND FERTILE | ||
|---|---|---|---|---|
| SEMEN | SWIM-UP | FERTILE | ||
| 1 | 13.0 | 7.2 | 6.6 | 0.6 |
| 2 | 9.4 | 6.4 | 6.2 | 0.2 |
| 3 | 21.0 | 9.0 | 5.5 | 3.5 |
| 4 | 23.0 | 16.0 | 9.5 | 6.5 |
| 5 | 16.0 | 13.0 | 10.0 | 3.0 |
| 6 | 11.0 | 1.4 | 0.7 | 0.7 |
| 7 | 22.0 | 12.3 | 10.3 | 2.0 |
| 8 | 16.0 | 4.5 | 3.4 | 1.1 |
| 9 | 43.0 | 14.0 | 11.0 | 3.0 |
| 10 | 8.0 | 3.0 | 2.7 | 0.3 |
| Ø | 18.2 | 8.7 | 6.6 | 2.1 |
List of all patients
| PATIENT | AGE | CONCENTRATION (MIL/ML) | MOTILITY (%) | NORMAL MORPHOLOGY (%) | DIAGNOSIS |
|---|---|---|---|---|---|
| 1 | 27 | 42 | 62 | 11 | Normozoospermia |
| 2 | 30 | 27 | 43 | 6 | Normozoospermia |
| 3 | 38 | 37 | 54 | 12 | Normozoospermia |
| 4 | 29 | 45 | 64 | 15 | Normozoospermia |
| 5 | 32 | 49 | 45 | 13 | Normozoospermia |
| 6 | 33 | 104 | 61 | 12 | Normozoospermia |
| 7 | 37 | 34 | 28 | 7 | Asthenozoospermia |
| 8 | 40 | 78 | 67 | 11 | Normozoospermia |
| 9 | 35 | 56 | 39 | 5 | Asthenozoospermia |
| 10 | 27 | 38 | 48 | 9 | Normozoospermia |