Table 1
List of all patients
| PATIENT | AGE | CONCENTRATION (MIL/ML) | MOTILITY (%) | NORMAL MORPHOLOGY (%) | DIAGNOSIS |
|---|---|---|---|---|---|
| 1 | 27 | 42 | 62 | 11 | Normozoospermia |
| 2 | 30 | 27 | 43 | 6 | Normozoospermia |
| 3 | 38 | 37 | 54 | 12 | Normozoospermia |
| 4 | 29 | 45 | 64 | 15 | Normozoospermia |
| 5 | 32 | 49 | 45 | 13 | Normozoospermia |
| 6 | 33 | 104 | 61 | 12 | Normozoospermia |
| 7 | 37 | 34 | 28 | 7 | Asthenozoospermia |
| 8 | 40 | 78 | 67 | 11 | Normozoospermia |
| 9 | 35 | 56 | 39 | 5 | Asthenozoospermia |
| 10 | 27 | 38 | 48 | 9 | Normozoospermia |
Figure 1
Graphical presentation of separation efficiency by the swim-up and FERTILE methods. Statistically significant differences are indicated by different symbols (A, B) (P< 0.05)
Table 2
DFI values of all patients before and after separation by swim-up and FERTILE method
| PATIENT | DFI (%; ABSOLUTE VALUE) | DIFFERENCES BETWEEN SWIM-UP AND FERTILE | ||
|---|---|---|---|---|
| SEMEN | SWIM-UP | FERTILE | ||
| 1 | 13.0 | 7.2 | 6.6 | 0.6 |
| 2 | 9.4 | 6.4 | 6.2 | 0.2 |
| 3 | 21.0 | 9.0 | 5.5 | 3.5 |
| 4 | 23.0 | 16.0 | 9.5 | 6.5 |
| 5 | 16.0 | 13.0 | 10.0 | 3.0 |
| 6 | 11.0 | 1.4 | 0.7 | 0.7 |
| 7 | 22.0 | 12.3 | 10.3 | 2.0 |
| 8 | 16.0 | 4.5 | 3.4 | 1.1 |
| 9 | 43.0 | 14.0 | 11.0 | 3.0 |
| 10 | 8.0 | 3.0 | 2.7 | 0.3 |
| Ø | 18.2 | 8.7 | 6.6 | 2.1 |
Table 3
Demonstration of efficiency of the separation methods. Ability of the separation methods to reduce the number of spermatozoa with fragmented DNA was evaluated, the initial DFI value of the unprocessed ejaculate is considered 100%
| PATIENT | DFI (%; RELATIVE VALUE) | DIFFERENCES BETWEEN SWIM-UP AND FERTILE | ||
|---|---|---|---|---|
| SEMEN | SWIM-UP | FERTILE | ||
| 1 | 100 | 55.0 | 50.7 | 4.3 |
| 2 | 100 | 68.0 | 66.0 | 2.0 |
| 3 | 100 | 42.8 | 26.2 | 16.0 |
| 4 | 100 | 69.0 | 41.3 | 27.7 |
| 5 | 100 | 81.0 | 62.5 | 18.5 |
| 6 | 100 | 15.4 | 6.3 | 9.1 |
| 7 | 100 | 55.9 | 46.8 | 9.1 |
| 8 | 100 | 28.1 | 21.3 | 6.8 |
| 9 | 100 | 32.6 | 25.5 | 7.1 |
| 10 | 100 | 37.5 | 33.7 | 3.8 |
| Ø | 100 | 48.5 | 38.0 | 10.5 |