Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

Lisa Moncrieff; Ievgeniia Kocherova; Artur Bryja; Wiesława Kranc; Joanna Perek; Magdalena Kulus; Michal Jeseta; Claudia Dompe; Greg Hutchings; Krzysztof Janowicz; Piotr Celichowski; Małgorzata Bruska; Maciej Zabel; Bartosz Kempisty; Paul Mozdziak

doi:10.2478/acb-2020-0001

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Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

Medical Journal of Cell Biology

Volume 8 (2020): Issue 1 (March 2020)

By: Lisa Moncrieff, Ievgeniia Kocherova, Artur Bryja, Wiesława Kranc, Joanna Perek, Magdalena Kulus, Michal Jeseta, Claudia Dompe, Greg Hutchings, Krzysztof Janowicz, Piotr Celichowski, Małgorzata Bruska, Maciej Zabel, Bartosz Kempisty and Paul Mozdziak

Open Access

|Apr 2020

Abstract

The growth and development of the oocyte is essential for the ovarian follicle. Cumulus cells (CCs) - a population of granulosa cells - exchange metabolites, proteins and oocyte-derived paracrine factors with the oocyte through gap junctions, to contribute to the competency and health of the oocyte. This bi-directional communication of the cumulus-oocyte complex could be better understood through the micro-analysis of a porcine oocyte gene expression before in vitro maturation (IVM) and after. Additionally, the study of the somatic and gamete cells differentiation capability into neuronal lineage would be promising for future stem cell research as granulosa cells are easily accessible waste material from in vitro fertilization (IVF) procedures. Therefore, in this study, the oocytes of 45 pubertal Landrace gilts were isolated and the protein expression of the COCs were analyzed through micro-analysis techniques. Genes belonging to two ontological groups: neuron differentiation and negative regulation of cell differentiation have been identified which have roles in proliferation, migration and differentiation. Twenty identified porcine oocyte genes (VEGFA, BTG2, MCOLN3, EGR2, TGFBR3, GJA1, FST, CTNNA2, RTN4, MDGA1, KIT, RYK, NOTCH2, RORA, SMAD4, ITGB1, SEMA5A, SMARCA1, WWTR1 and APP) were found to be down-regulated after the transition of IVM compared to in vitro. These results could be applied as gene markers for the proliferation, migration and differentiation occurring in the bi-directional communication between the oocyte and CCs.

Running title: Differentiation and neurogenesis in oocyte cells

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DOI: https://doi.org/10.2478/acb-2020-0001 | Journal eISSN: 2544-3577

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Language: English

Page range: 1 - 11

Submitted on: Dec 19, 2019

Accepted on: Feb 5, 2020

Published on: Apr 29, 2020

Published by: Foundation for Cell Biology and Molecular Biology

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

pig,

oocyte,

cumulus-oocyte complex,

Related subjects:

© 2020 Lisa Moncrieff, Ievgeniia Kocherova, Artur Bryja, Wiesława Kranc, Joanna Perek, Magdalena Kulus, Michal Jeseta, Claudia Dompe, Greg Hutchings, Krzysztof Janowicz, Piotr Celichowski, Małgorzata Bruska, Maciej Zabel, Bartosz Kempisty, Paul Mozdziak, published by Foundation for Cell Biology and Molecular Biology
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Volume 8 (2020): Issue 1 (March 2020)