Biochemical properties of cofactor and coenzyme metabolism in porcine oviductal epithelial cells – a microarray study

Ievgeniia Kocherova; Magdalena Kulus; Claudia Dompe; Paweł Antosik; Dorota Bukowska; Bartosz Kempisty; Paul Mozdziak

doi:10.2478/acb-2019-0017

Figures & Tables

Heat map representation of differentially expressed genes belonging to the to “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms. Arbitrary signal intensity acquired from microarray analysis is represented by colours (green, higher; red, lower expression). Log2 signal intensity values for any single gene were resized to Row Z-Score scale (from -2, the lowest expression to +2, the highest expression for single gene)

The circle plot showing the differently expressed genes and z-score of the “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms. The outer circle shows a scatter plot for each term of the fold change of the assigned genes. Green circles display up- regulation and red ones down- regulation. The inner circle shows the z-score of each GO BP term. The width of the each bar corresponds to the number of genes within GO BP term and the color corresponds to the z-score

The representation of the mutual relationship of differently expressed genes that belong to “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms. The ribbons indicate which gene belongs to which categories. The middle circle represents logarithm from fold change (LogFC) between D7/D1, D15/D1 and D30/D1 respectively. The color of each block corresponds to the LogFC of each gene (green – up-regulated, red – down-regulated). The genes were sorted by logFC from most to least changed gene

Heatmap showing the gene occurrence between chosen differently expressed genes that belongs to “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms. The yellow color is associated with gene occurrence in the GO Term. The intensity of the color is corresponding to amount of GO BP terms that each gene belongs to

STRING-generated interaction occurrence between chosen differently expressed genes that belongs to the to “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms. The intensity of the edges reflects the strength of interaction score

Functional interaction (FI) between chosen differently expressed genes that belongs to the to “coenzyme metabolic process”, “cofactor biosynthetic process” and “cofactor metabolic process” GO BP terms. In following figure “->” stands for activating/catalyzing, “-|” for inhibition, “-” for FIs extracted from complexes or inputs, and “---” for predicted FIs

Gene symbols, fold change in expression ratio, Entrez gene IDs, corrected p values and mean value of fold change ratio of studied genes

GENE SYMBOL	RATIO D7/D1	RATIO D15/D1	RATIO D30/D1	ADJUSTED P VALUE D7/D1	ADJUSTED P VALUE D15/D1	ADJUSTED P VALUE D30/D1	ENTREZ GENE ID	MEAN RATIO
ACSL4	2,078475	-1,049840	-1,389865	0,000888	0,778847	0,027679	448980	-0,120410
HAAO	2,260462	1,012229	-1,484548	0,000495	0,948252	0,010911	100514283	0,596048
PPARA	2,156741	1,463697	1,431760	0,001697	0,036412	0,040484	397239	1,684066
CPOX	2,562460	1,513475	1,395239	0,000490	0,019946	0,042725	100511474	1,823725
ALDOC	3,100575	1,215495	1,317360	0,000301	0,274540	0,110445	100512013	1,877810
PGM1	2,424519	1,680756	1,691567	0,000276	0,002924	0,002153	397566	1,932281
TPI1	2,751177	1,737387	1,421065	0,000059	0,000779	0,006133	100157582	1,969876
ME1	2,691975	1,528067	1,911722	0,000358	0,017206	0,001723	397538	2,043921
ACSL3	2,049555	1,676912	2,729973	0,002097	0,008605	0,000218	100233169	2,152147
FECH	2,109918	2,722032	2,420194	0,000678	0,000117	0,000152	100322873	2,417382
SLC25A1	3,272247	1,857686	2,239022	0,000043	0,000649	0,000117	100154310	2,456318
OXSM	2,234415	2,697009	2,514316	0,000560	0,000143	0,000144	---	2,481914
PFKFB4	2,978245	3,699473	2,573760	0,000132	0,000034	0,000122	100158056	3,083826
PDK1	4,856476	1,981458	2,658224	0,000100	0,004418	0,000537	100286871	3,165386
PCBD1	2,831266	3,234674	3,436204	0,000047	0,000015	0,000007	100155605	3,167382
ACACA	3,411749	3,730045	3,501388	0,000588	0,000307	0,000279	397324	3,547727
TSPO	3,123906	3,962945	3,669010	0,000048	0,000012	0,000009	396592	3,585287
ACOT13	2,203769	4,264528	4,968930	0,000233	0,000008	0,000003	100514587	3,812409
SCD	5,037882	1,402767	5,796847	0,000092	0,087483	0,000025	396670	4,079166
PDK3	2,773770	4,066151	5,486628	0,000182	0,000024	0,000006	100153858	4,108850
AASS	2,332931	4,119242	6,132447	0,007329	0,000476	0,000092	100513962	4,194873
ACSS2	6,349718	2,503393	5,620930	0,000007	0,000095	0,000002	100153866	4,824680
PANK1	7,855562	3,491674	7,158650	0,000007	0,000033	0,000002	100154650	6,168629
P2RX7	6,165142	8,522042	5,795398	0,000027	0,000007	0,000010	497623	6,827528
ALDH1L2	4,535793	8,626347	22,102755	0,000038	0,000004	0,000001	100151976	11,754965

Biochemical properties of cofactor and coenzyme metabolism in porcine oviductal epithelial cells – a microarray study

Figures & Tables

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Gene symbols, fold change in expression ratio, Entrez gene IDs, corrected p values and mean value of fold change ratio of studied genes

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